Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3400 and GFF3401 are separated by 124 nucleotides GFF3401 and GFF3402 are separated by 17 nucleotides GFF3402 and GFF3403 overlap by 4 nucleotides GFF3403 and GFF3404 are separated by 151 nucleotides
GFF3400 - Benzaldehyde dehydrogenase, at 223,380 to 224,930
GFF3400
GFF3401 - P-hydroxylaminobenzoate lyase, at 225,055 to 225,546
GFF3401
GFF3402 - no description, at 225,564 to 225,713
GFF3402
GFF3403 - Benzoate catabolism regulator with shikimate kinase domain, at 225,710 to 226,654
GFF3403
GFF3404 - Benzoyl-CoA-dihydrodiol lyase (EC 4.1.2.44), at 226,806 to 228,482
GFF3404
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 224.746 kb on + strand, within GFF3400 at 224.746 kb on + strand, within GFF3400 at 224.747 kb on - strand, within GFF3400 at 224.747 kb on - strand, within GFF3400 at 224.908 kb on + strand at 224.908 kb on + strand at 224.908 kb on + strand at 224.908 kb on + strand at 224.908 kb on + strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 224.909 kb on - strand at 225.051 kb on + strand at 225.051 kb on + strand at 225.051 kb on + strand at 225.052 kb on - strand at 225.052 kb on - strand at 225.052 kb on - strand at 225.052 kb on - strand at 225.052 kb on - strand at 225.058 kb on + strand at 225.058 kb on + strand at 225.058 kb on + strand at 225.058 kb on + strand at 225.059 kb on - strand at 225.059 kb on - strand at 225.059 kb on - strand at 225.059 kb on - strand at 225.092 kb on + strand at 225.092 kb on + strand at 225.093 kb on - strand at 225.093 kb on - strand at 225.093 kb on - strand at 225.323 kb on + strand, within GFF3401 at 225.324 kb on - strand, within GFF3401 at 225.563 kb on + strand at 225.563 kb on + strand at 225.563 kb on + strand at 225.564 kb on - strand at 225.570 kb on + strand at 225.570 kb on + strand at 225.570 kb on + strand at 225.570 kb on + strand at 225.570 kb on + strand at 225.571 kb on - strand at 225.571 kb on - strand at 225.571 kb on - strand at 225.571 kb on - strand at 225.571 kb on - strand at 225.619 kb on + strand, within GFF3402 at 225.619 kb on + strand, within GFF3402 at 225.619 kb on + strand, within GFF3402 at 225.620 kb on - strand, within GFF3402 at 225.620 kb on - strand, within GFF3402 at 225.620 kb on - strand, within GFF3402 at 225.620 kb on - strand, within GFF3402 at 225.723 kb on + strand at 225.723 kb on + strand at 225.723 kb on + strand at 225.723 kb on + strand at 225.724 kb on - strand at 225.724 kb on - strand at 225.724 kb on - strand at 225.724 kb on - strand at 225.917 kb on + strand, within GFF3403 at 225.918 kb on - strand, within GFF3403 at 225.918 kb on - strand, within GFF3403 at 225.918 kb on - strand, within GFF3403 at 226.280 kb on + strand, within GFF3403 at 226.280 kb on + strand, within GFF3403 at 226.280 kb on + strand, within GFF3403 at 226.281 kb on - strand, within GFF3403 at 226.281 kb on - strand, within GFF3403 at 226.281 kb on - strand, within GFF3403 at 226.281 kb on - strand, within GFF3403 at 226.281 kb on - strand, within GFF3403 at 226.296 kb on - strand, within GFF3403 at 226.304 kb on + strand, within GFF3403 at 226.304 kb on + strand, within GFF3403 at 226.304 kb on + strand, within GFF3403 at 226.340 kb on + strand, within GFF3403 at 226.340 kb on + strand, within GFF3403 at 226.340 kb on + strand, within GFF3403 at 226.340 kb on + strand, within GFF3403 at 226.340 kb on + strand, within GFF3403 at 226.341 kb on - strand, within GFF3403 at 226.341 kb on - strand, within GFF3403 at 226.341 kb on - strand, within GFF3403 at 226.553 kb on + strand, within GFF3403 at 226.553 kb on + strand, within GFF3403 at 226.553 kb on + strand, within GFF3403 at 226.553 kb on + strand, within GFF3403 at 226.553 kb on + strand, within GFF3403 at 226.554 kb on - strand, within GFF3403 at 226.554 kb on - strand, within GFF3403 at 226.652 kb on + strand at 226.848 kb on + strand at 226.849 kb on - strand at 226.870 kb on - strand at 226.953 kb on + strand at 226.974 kb on + strand, within GFF3404 at 226.974 kb on + strand, within GFF3404 at 226.974 kb on + strand, within GFF3404 at 226.974 kb on + strand, within GFF3404 at 226.974 kb on + strand, within GFF3404 at 226.974 kb on + strand, within GFF3404 at 226.975 kb on - strand, within GFF3404 at 226.975 kb on - strand, within GFF3404 at 226.975 kb on - strand, within GFF3404 at 226.975 kb on - strand, within GFF3404 at 226.975 kb on - strand, within GFF3404 at 227.245 kb on - strand, within GFF3404 at 227.604 kb on + strand, within GFF3404
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 224,746 + GFF3400 0.88 -0.1 224,746 + GFF3400 0.88 -0.4 224,747 - GFF3400 0.88 -0.2 224,747 - GFF3400 0.88 -0.5 224,908 + +0.3 224,908 + +0.3 224,908 + +0.6 224,908 + -0.9 224,908 + +0.6 224,909 - +1.1 224,909 - -1.2 224,909 - +0.6 224,909 - -0.7 224,909 - -0.5 224,909 - -0.9 224,909 - -0.5 224,909 - -1.6 225,051 + -0.9 225,051 + -0.5 225,051 + -0.4 225,052 - -0.7 225,052 - -1.3 225,052 - -1.3 225,052 - -1.3 225,052 - +0.2 225,058 + -0.1 225,058 + -0.2 225,058 + -0.6 225,058 + +1.8 225,059 - +1.8 225,059 - -0.2 225,059 - -1.2 225,059 - +1.0 225,092 + -0.9 225,092 + -0.4 225,093 - -0.5 225,093 - +0.3 225,093 - -2.1 225,323 + GFF3401 0.54 -0.4 225,324 - GFF3401 0.55 +0.8 225,563 + -1.5 225,563 + +0.4 225,563 + +0.6 225,564 - -0.2 225,570 + +0.3 225,570 + -0.9 225,570 + -3.2 225,570 + -2.4 225,570 + -1.0 225,571 - -1.5 225,571 - -1.5 225,571 - -1.0 225,571 - -1.3 225,571 - -1.0 225,619 + GFF3402 0.37 -0.2 225,619 + GFF3402 0.37 -0.7 225,619 + GFF3402 0.37 +1.3 225,620 - GFF3402 0.37 +2.1 225,620 - GFF3402 0.37 -0.5 225,620 - GFF3402 0.37 +0.8 225,620 - GFF3402 0.37 -1.9 225,723 + -0.2 225,723 + +0.5 225,723 + -1.2 225,723 + -1.1 225,724 - -0.1 225,724 - +0.7 225,724 - +0.7 225,724 - -0.4 225,917 + GFF3403 0.22 -1.7 225,918 - GFF3403 0.22 -1.3 225,918 - GFF3403 0.22 -0.2 225,918 - GFF3403 0.22 -0.2 226,280 + GFF3403 0.60 -1.9 226,280 + GFF3403 0.60 -0.3 226,280 + GFF3403 0.60 -0.7 226,281 - GFF3403 0.60 -1.2 226,281 - GFF3403 0.60 -1.4 226,281 - GFF3403 0.60 -0.7 226,281 - GFF3403 0.60 -0.6 226,281 - GFF3403 0.60 -0.8 226,296 - GFF3403 0.62 -0.6 226,304 + GFF3403 0.63 -2.2 226,304 + GFF3403 0.63 -0.6 226,304 + GFF3403 0.63 -1.5 226,340 + GFF3403 0.67 -2.4 226,340 + GFF3403 0.67 +0.8 226,340 + GFF3403 0.67 -0.9 226,340 + GFF3403 0.67 -0.7 226,340 + GFF3403 0.67 -1.2 226,341 - GFF3403 0.67 -2.2 226,341 - GFF3403 0.67 +0.5 226,341 - GFF3403 0.67 +0.6 226,553 + GFF3403 0.89 +2.1 226,553 + GFF3403 0.89 +0.3 226,553 + GFF3403 0.89 -1.3 226,553 + GFF3403 0.89 -0.9 226,553 + GFF3403 0.89 +0.3 226,554 - GFF3403 0.89 -0.1 226,554 - GFF3403 0.89 -1.3 226,652 + +0.8 226,848 + -2.0 226,849 - -0.5 226,870 - -1.3 226,953 + -0.2 226,974 + GFF3404 0.10 -1.7 226,974 + GFF3404 0.10 +0.1 226,974 + GFF3404 0.10 +0.5 226,974 + GFF3404 0.10 -0.1 226,974 + GFF3404 0.10 +2.4 226,974 + GFF3404 0.10 -1.8 226,975 - GFF3404 0.10 -0.4 226,975 - GFF3404 0.10 +0.6 226,975 - GFF3404 0.10 -0.5 226,975 - GFF3404 0.10 -1.4 226,975 - GFF3404 0.10 -1.7 227,245 - GFF3404 0.26 +1.0 227,604 + GFF3404 0.48 -1.4
Or see this region's nucleotide sequence