Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3253 and GFF3254 are separated by 236 nucleotides GFF3254 and GFF3255 are separated by 69 nucleotides GFF3255 and GFF3256 are separated by 83 nucleotides
GFF3253 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 69,528 to 72,122
GFF3253
GFF3254 - Phosphotransferase involved in threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 72,359 to 73,456
GFF3254
GFF3255 - 16S rRNA (uracil(1498)-N(3))-methyltransferase (EC 2.1.1.193), at 73,526 to 74,263
GFF3255
GFF3256 - Peptidase, M20/M25/M40 family, at 74,347 to 75,702
GFF3256
Position (kb) 72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 72.345 kb on - strand at 72.345 kb on - strand at 72.345 kb on - strand at 72.355 kb on - strand at 72.357 kb on - strand at 72.533 kb on + strand, within GFF3254 at 72.533 kb on + strand, within GFF3254 at 72.534 kb on - strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.776 kb on + strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.777 kb on - strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.842 kb on + strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.843 kb on - strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.890 kb on + strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.891 kb on - strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.995 kb on + strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 72.996 kb on - strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.026 kb on + strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.027 kb on - strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.079 kb on + strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.080 kb on - strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.094 kb on + strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.095 kb on - strand, within GFF3254 at 73.226 kb on + strand, within GFF3254 at 73.226 kb on + strand, within GFF3254 at 73.226 kb on + strand, within GFF3254 at 73.227 kb on - strand, within GFF3254 at 73.227 kb on - strand, within GFF3254 at 73.227 kb on - strand, within GFF3254 at 73.316 kb on + strand, within GFF3254 at 73.317 kb on - strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.346 kb on + strand, within GFF3254 at 73.347 kb on - strand at 73.347 kb on - strand at 73.347 kb on - strand at 73.347 kb on - strand at 73.347 kb on - strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.370 kb on + strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.371 kb on - strand at 73.442 kb on + strand at 73.442 kb on + strand at 73.491 kb on + strand at 73.492 kb on + strand at 73.492 kb on + strand at 73.492 kb on + strand at 73.493 kb on - strand at 73.664 kb on + strand, within GFF3255 at 73.664 kb on + strand, within GFF3255 at 73.664 kb on + strand, within GFF3255 at 73.665 kb on - strand, within GFF3255 at 73.665 kb on - strand, within GFF3255 at 73.665 kb on - strand, within GFF3255 at 73.665 kb on - strand, within GFF3255 at 74.074 kb on + strand, within GFF3255 at 74.075 kb on - strand, within GFF3255 at 74.312 kb on + strand at 74.312 kb on + strand at 74.312 kb on + strand at 74.312 kb on + strand at 74.312 kb on + strand at 74.312 kb on + strand at 74.312 kb on + strand at 74.313 kb on - strand at 74.313 kb on - strand at 74.313 kb on - strand at 74.320 kb on + strand at 74.320 kb on + strand at 74.320 kb on + strand at 74.320 kb on + strand at 74.320 kb on + strand at 74.321 kb on - strand at 74.321 kb on - strand at 74.321 kb on - strand at 74.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 72,345 - -0.6 72,345 - +0.5 72,345 - -0.3 72,355 - -0.8 72,357 - -0.1 72,533 + GFF3254 0.16 +0.5 72,533 + GFF3254 0.16 -0.2 72,534 - GFF3254 0.16 -2.0 72,776 + GFF3254 0.38 -0.7 72,776 + GFF3254 0.38 -0.8 72,776 + GFF3254 0.38 -1.1 72,776 + GFF3254 0.38 -0.6 72,776 + GFF3254 0.38 -1.0 72,777 - GFF3254 0.38 +0.3 72,777 - GFF3254 0.38 -2.7 72,777 - GFF3254 0.38 -0.6 72,777 - GFF3254 0.38 -1.9 72,777 - GFF3254 0.38 -2.0 72,842 + GFF3254 0.44 -0.7 72,842 + GFF3254 0.44 -0.4 72,842 + GFF3254 0.44 -0.1 72,843 - GFF3254 0.44 -0.5 72,843 - GFF3254 0.44 -1.6 72,843 - GFF3254 0.44 -0.5 72,843 - GFF3254 0.44 -1.0 72,843 - GFF3254 0.44 -2.4 72,890 + GFF3254 0.48 -1.2 72,890 + GFF3254 0.48 -0.9 72,890 + GFF3254 0.48 -1.3 72,890 + GFF3254 0.48 -1.0 72,890 + GFF3254 0.48 -1.5 72,890 + GFF3254 0.48 -0.4 72,890 + GFF3254 0.48 -2.8 72,890 + GFF3254 0.48 -1.6 72,890 + GFF3254 0.48 +0.3 72,890 + GFF3254 0.48 -1.3 72,890 + GFF3254 0.48 -0.9 72,890 + GFF3254 0.48 +0.3 72,890 + GFF3254 0.48 -1.4 72,890 + GFF3254 0.48 -0.3 72,891 - GFF3254 0.48 -0.6 72,891 - GFF3254 0.48 -0.9 72,891 - GFF3254 0.48 -0.7 72,891 - GFF3254 0.48 -0.7 72,891 - GFF3254 0.48 -0.5 72,891 - GFF3254 0.48 -0.3 72,891 - GFF3254 0.48 -2.7 72,891 - GFF3254 0.48 +0.3 72,891 - GFF3254 0.48 -1.2 72,891 - GFF3254 0.48 -1.4 72,891 - GFF3254 0.48 -1.1 72,891 - GFF3254 0.48 -1.2 72,995 + GFF3254 0.58 -0.1 72,995 + GFF3254 0.58 +1.8 72,995 + GFF3254 0.58 -1.6 72,995 + GFF3254 0.58 -2.2 72,995 + GFF3254 0.58 -1.2 72,995 + GFF3254 0.58 -0.3 72,995 + GFF3254 0.58 -2.8 72,995 + GFF3254 0.58 -1.1 72,995 + GFF3254 0.58 -1.1 72,995 + GFF3254 0.58 +0.0 72,996 - GFF3254 0.58 -0.7 72,996 - GFF3254 0.58 -1.5 72,996 - GFF3254 0.58 -1.8 72,996 - GFF3254 0.58 -1.5 72,996 - GFF3254 0.58 -1.9 72,996 - GFF3254 0.58 -2.4 73,026 + GFF3254 0.61 -1.9 73,026 + GFF3254 0.61 -1.5 73,026 + GFF3254 0.61 +1.2 73,026 + GFF3254 0.61 -0.9 73,026 + GFF3254 0.61 -1.5 73,026 + GFF3254 0.61 -0.2 73,026 + GFF3254 0.61 -0.8 73,027 - GFF3254 0.61 -0.9 73,027 - GFF3254 0.61 -1.0 73,027 - GFF3254 0.61 -0.7 73,027 - GFF3254 0.61 -0.6 73,027 - GFF3254 0.61 -1.6 73,027 - GFF3254 0.61 -1.3 73,027 - GFF3254 0.61 +0.5 73,079 + GFF3254 0.66 -2.1 73,079 + GFF3254 0.66 -1.1 73,079 + GFF3254 0.66 -0.7 73,080 - GFF3254 0.66 -1.1 73,080 - GFF3254 0.66 -0.8 73,080 - GFF3254 0.66 -1.6 73,094 + GFF3254 0.67 -2.6 73,094 + GFF3254 0.67 +0.1 73,094 + GFF3254 0.67 -1.9 73,094 + GFF3254 0.67 +1.8 73,094 + GFF3254 0.67 -1.0 73,094 + GFF3254 0.67 +1.8 73,094 + GFF3254 0.67 -0.7 73,094 + GFF3254 0.67 -1.9 73,094 + GFF3254 0.67 -0.3 73,094 + GFF3254 0.67 -2.0 73,095 - GFF3254 0.67 +0.3 73,095 - GFF3254 0.67 -0.3 73,095 - GFF3254 0.67 -1.7 73,095 - GFF3254 0.67 -1.4 73,095 - GFF3254 0.67 -0.3 73,095 - GFF3254 0.67 -2.2 73,095 - GFF3254 0.67 -1.7 73,095 - GFF3254 0.67 -1.7 73,095 - GFF3254 0.67 -1.4 73,095 - GFF3254 0.67 -0.2 73,095 - GFF3254 0.67 -2.2 73,226 + GFF3254 0.79 -1.3 73,226 + GFF3254 0.79 -0.0 73,226 + GFF3254 0.79 -1.3 73,227 - GFF3254 0.79 +1.0 73,227 - GFF3254 0.79 -1.3 73,227 - GFF3254 0.79 -0.7 73,316 + GFF3254 0.87 -0.9 73,317 - GFF3254 0.87 -0.5 73,346 + GFF3254 0.90 -0.7 73,346 + GFF3254 0.90 -1.2 73,346 + GFF3254 0.90 -1.0 73,346 + GFF3254 0.90 -2.0 73,346 + GFF3254 0.90 +1.8 73,346 + GFF3254 0.90 -0.8 73,346 + GFF3254 0.90 +0.0 73,346 + GFF3254 0.90 -0.3 73,346 + GFF3254 0.90 -0.4 73,347 - -0.6 73,347 - -0.2 73,347 - -0.6 73,347 - +0.0 73,347 - -1.1 73,370 + -1.7 73,370 + -0.6 73,370 + +1.4 73,370 + -0.4 73,370 + -0.3 73,370 + -1.0 73,370 + -0.7 73,370 + -2.3 73,371 - -0.2 73,371 - -1.0 73,371 - -0.5 73,371 - -0.2 73,371 - -1.5 73,371 - -0.5 73,371 - -0.7 73,371 - -1.2 73,442 + +1.6 73,442 + +1.4 73,491 + -1.0 73,492 + -0.7 73,492 + -0.2 73,492 + +1.4 73,493 - +0.9 73,664 + GFF3255 0.19 +0.4 73,664 + GFF3255 0.19 +0.5 73,664 + GFF3255 0.19 -0.3 73,665 - GFF3255 0.19 +0.1 73,665 - GFF3255 0.19 -1.0 73,665 - GFF3255 0.19 -0.6 73,665 - GFF3255 0.19 +0.8 74,074 + GFF3255 0.74 -0.5 74,075 - GFF3255 0.74 +0.5 74,312 + +0.3 74,312 + +0.3 74,312 + -1.2 74,312 + +0.1 74,312 + -0.1 74,312 + -0.0 74,312 + -0.6 74,313 - -0.7 74,313 - -0.5 74,313 - +0.6 74,320 + +0.0 74,320 + -0.0 74,320 + -1.2 74,320 + -0.5 74,320 + +0.1 74,321 - -0.8 74,321 - +0.3 74,321 - +1.5 74,321 - -0.2
Or see this region's nucleotide sequence