Strain Fitness in Variovorax sp. SCN45 around GFF320

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF318 and GFF319 are separated by 17 nucleotidesGFF319 and GFF320 overlap by 4 nucleotidesGFF320 and GFF321 overlap by 19 nucleotidesGFF321 and GFF322 are separated by 33 nucleotides GFF318 - Uncharacterized protein YjgR, at 350,964 to 352,481 GFF318 GFF319 - EXTRACYTOPLASMIC FUNCTION ALTERNATIVE SIGMA FACTOR, at 352,499 to 353,140 GFF319 GFF320 - RNA polymerase ECF-type sigma factor, at 353,137 to 353,727 GFF320 GFF321 - hypothetical protein, at 353,709 to 353,975 GFF321 GFF322 - GTP cyclohydrolase II homolog, at 354,009 to 355,151 GFF322 Position (kb) 353 354Strain fitness (log2 ratio) -2 -1 0 1 2at 352.212 kb on + strand, within GFF318at 352.213 kb on - strand, within GFF318at 352.213 kb on - strand, within GFF318at 352.213 kb on - strand, within GFF318at 352.340 kb on + strandat 352.500 kb on + strandat 352.500 kb on + strandat 352.500 kb on + strandat 352.501 kb on - strandat 352.566 kb on + strand, within GFF319at 352.566 kb on + strand, within GFF319at 352.567 kb on - strand, within GFF319at 352.567 kb on - strand, within GFF319at 352.567 kb on - strand, within GFF319at 352.567 kb on - strand, within GFF319at 352.567 kb on - strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.614 kb on + strand, within GFF319at 352.615 kb on - strand, within GFF319at 352.615 kb on - strand, within GFF319at 353.007 kb on + strand, within GFF319at 353.008 kb on - strand, within GFF319at 353.008 kb on - strand, within GFF319at 353.008 kb on - strand, within GFF319at 353.283 kb on + strand, within GFF320at 353.283 kb on + strand, within GFF320at 353.283 kb on + strand, within GFF320at 353.284 kb on - strand, within GFF320at 353.284 kb on - strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.447 kb on + strand, within GFF320at 353.448 kb on - strand, within GFF320at 353.448 kb on - strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.495 kb on + strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.496 kb on - strand, within GFF320at 353.710 kb on + strandat 353.710 kb on + strandat 353.710 kb on + strandat 353.711 kb on - strandat 353.711 kb on - strandat 353.711 kb on - strandat 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.815 kb on + strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 353.816 kb on - strand, within GFF321at 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.049 kb on + strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.050 kb on - strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.069 kb on + strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.070 kb on - strandat 354.079 kb on - strandat 354.079 kb on - strandat 354.261 kb on + strand, within GFF322at 354.261 kb on + strand, within GFF322at 354.417 kb on + strand, within GFF322at 354.418 kb on - strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.682 kb on - strand, within GFF322

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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352,212 + GFF318 0.82 -1.1
352,213 - GFF318 0.82 -0.0
352,213 - GFF318 0.82 +0.0
352,213 - GFF318 0.82 -0.1
352,340 + -0.0
352,500 + +0.6
352,500 + -0.5
352,500 + -0.9
352,501 - +0.3
352,566 + GFF319 0.10 +1.2
352,566 + GFF319 0.10 -0.1
352,567 - GFF319 0.11 +0.5
352,567 - GFF319 0.11 -0.5
352,567 - GFF319 0.11 -0.4
352,567 - GFF319 0.11 +0.3
352,567 - GFF319 0.11 +0.5
352,614 + GFF319 0.18 -0.5
352,614 + GFF319 0.18 +0.2
352,614 + GFF319 0.18 -0.2
352,614 + GFF319 0.18 +0.4
352,614 + GFF319 0.18 +2.5
352,614 + GFF319 0.18 +0.5
352,614 + GFF319 0.18 -0.3
352,614 + GFF319 0.18 -0.8
352,615 - GFF319 0.18 +1.0
352,615 - GFF319 0.18 -0.6
353,007 + GFF319 0.79 -0.1
353,008 - GFF319 0.79 -1.7
353,008 - GFF319 0.79 -1.1
353,008 - GFF319 0.79 +1.2
353,283 + GFF320 0.25 -0.3
353,283 + GFF320 0.25 -0.6
353,283 + GFF320 0.25 -0.9
353,284 - GFF320 0.25 +1.7
353,284 - GFF320 0.25 +0.3
353,447 + GFF320 0.52 -0.6
353,447 + GFF320 0.52 -1.1
353,447 + GFF320 0.52 -0.0
353,448 - GFF320 0.53 -1.1
353,448 - GFF320 0.53 -1.2
353,495 + GFF320 0.61 -0.9
353,495 + GFF320 0.61 -0.8
353,495 + GFF320 0.61 +0.6
353,495 + GFF320 0.61 -0.4
353,496 - GFF320 0.61 -0.5
353,496 - GFF320 0.61 +2.4
353,496 - GFF320 0.61 -1.6
353,710 + -0.6
353,710 + -0.8
353,710 + -0.6
353,711 - +1.1
353,711 - -0.2
353,711 - -0.1
353,815 + GFF321 0.40 -0.2
353,815 + GFF321 0.40 -1.9
353,815 + GFF321 0.40 +0.0
353,815 + GFF321 0.40 -0.7
353,815 + GFF321 0.40 +0.9
353,815 + GFF321 0.40 -0.9
353,815 + GFF321 0.40 +0.6
353,815 + GFF321 0.40 -0.3
353,816 - GFF321 0.40 -0.2
353,816 - GFF321 0.40 -0.9
353,816 - GFF321 0.40 +0.6
353,816 - GFF321 0.40 -1.1
353,816 - GFF321 0.40 +0.2
353,816 - GFF321 0.40 -0.1
354,049 + +0.2
354,049 + +0.2
354,049 + -1.2
354,049 + -1.4
354,049 + -0.8
354,049 + -0.1
354,049 + -0.6
354,050 - -0.3
354,050 - -0.4
354,050 - +0.1
354,050 - +0.3
354,050 - -0.1
354,050 - -0.5
354,050 - -0.7
354,069 + -0.2
354,069 + +0.2
354,069 + +0.0
354,069 + -1.8
354,070 - +0.6
354,070 - -0.3
354,070 - +1.0
354,070 - +0.0
354,070 - -0.2
354,070 - -1.4
354,070 - +0.2
354,079 - +0.9
354,079 - -0.3
354,261 + GFF322 0.22 +0.1
354,261 + GFF322 0.22 -1.0
354,417 + GFF322 0.36 +0.2
354,418 - GFF322 0.36 +1.0
354,498 + GFF322 0.43 -0.4
354,498 + GFF322 0.43 -0.7
354,498 + GFF322 0.43 +1.1
354,499 - GFF322 0.43 -2.1
354,499 - GFF322 0.43 -0.0
354,499 - GFF322 0.43 -2.2
354,499 - GFF322 0.43 +0.4
354,564 + GFF322 0.49 -1.9
354,564 + GFF322 0.49 +0.9
354,564 + GFF322 0.49 -0.2
354,564 + GFF322 0.49 -1.4
354,564 + GFF322 0.49 +0.1
354,564 + GFF322 0.49 +0.1
354,565 - GFF322 0.49 +0.3
354,565 - GFF322 0.49 -0.4
354,565 - GFF322 0.49 -1.0
354,565 - GFF322 0.49 -0.8
354,565 - GFF322 0.49 -0.7
354,681 + GFF322 0.59 +0.9
354,681 + GFF322 0.59 -0.4
354,682 - GFF322 0.59 +0.2

Or see this region's nucleotide sequence