Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3139 and GFF3140 are separated by 748 nucleotides GFF3140 and GFF3141 are separated by 132 nucleotides
GFF3139 - NAD-dependent protein deacetylase of SIR2 family, at 570,437 to 571,153
GFF3139
GFF3140 - no description, at 571,902 to 572,111
GFF3140
GFF3141 - Phosphate acetyltransferase (EC 2.3.1.8), at 572,244 to 573,197
GFF3141
Position (kb) 571
572
573 Strain fitness (log2 ratio) -2
-1
0
1 at 570.978 kb on + strand, within GFF3139 at 570.978 kb on + strand, within GFF3139 at 570.978 kb on + strand, within GFF3139 at 571.002 kb on + strand, within GFF3139 at 571.003 kb on - strand, within GFF3139 at 571.044 kb on + strand, within GFF3139 at 571.044 kb on + strand, within GFF3139 at 571.045 kb on - strand, within GFF3139 at 571.045 kb on - strand, within GFF3139 at 571.045 kb on - strand, within GFF3139 at 571.368 kb on - strand at 571.383 kb on - strand at 571.462 kb on + strand at 571.463 kb on - strand at 571.478 kb on - strand at 571.581 kb on + strand at 571.581 kb on + strand at 571.653 kb on + strand at 571.653 kb on + strand at 571.654 kb on - strand at 571.701 kb on + strand at 571.701 kb on + strand at 571.701 kb on + strand at 571.701 kb on + strand at 571.701 kb on + strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.702 kb on - strand at 571.903 kb on + strand at 572.086 kb on + strand, within GFF3140 at 572.086 kb on + strand, within GFF3140 at 572.087 kb on - strand, within GFF3140 at 572.087 kb on - strand, within GFF3140 at 572.150 kb on + strand at 572.151 kb on - strand at 572.197 kb on + strand at 572.197 kb on + strand at 572.198 kb on - strand at 572.198 kb on - strand at 572.212 kb on - strand at 572.277 kb on + strand at 572.277 kb on + strand at 572.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 570,978 + GFF3139 0.75 -0.1 570,978 + GFF3139 0.75 -0.4 570,978 + GFF3139 0.75 +0.1 571,002 + GFF3139 0.79 -0.0 571,003 - GFF3139 0.79 +0.3 571,044 + GFF3139 0.85 -1.0 571,044 + GFF3139 0.85 +0.5 571,045 - GFF3139 0.85 -0.8 571,045 - GFF3139 0.85 -1.0 571,045 - GFF3139 0.85 -0.6 571,368 - -0.5 571,383 - -0.8 571,462 + -0.0 571,463 - -0.3 571,478 - -0.5 571,581 + -1.2 571,581 + +0.3 571,653 + -0.6 571,653 + -1.8 571,654 - -0.3 571,701 + +1.3 571,701 + -0.7 571,701 + -2.7 571,701 + -2.4 571,701 + -0.4 571,702 - +1.3 571,702 - -0.8 571,702 - -0.1 571,702 - -0.9 571,702 - -0.6 571,702 - +0.1 571,702 - -2.5 571,702 - +0.3 571,702 - -1.2 571,903 + -0.2 572,086 + GFF3140 0.88 -0.9 572,086 + GFF3140 0.88 -0.1 572,087 - GFF3140 0.88 -0.2 572,087 - GFF3140 0.88 -1.1 572,150 + +0.1 572,151 - -0.3 572,197 + -0.5 572,197 + -0.1 572,198 - +0.3 572,198 - -0.2 572,212 - -0.5 572,277 + -1.5 572,277 + -0.2 572,278 - -0.6
Or see this region's nucleotide sequence