Strain Fitness in Variovorax sp. SCN45 around GFF3075

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3073 and GFF3074 are separated by 9 nucleotidesGFF3074 and GFF3075 are separated by 16 nucleotidesGFF3075 and GFF3076 are separated by 148 nucleotidesGFF3076 and GFF3077 are separated by 61 nucleotides GFF3073 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 506,004 to 506,978 GFF3073 GFF3074 - Putative polysaccharide deacetylase, at 506,988 to 507,773 GFF3074 GFF3075 - Surface antigen, at 507,790 to 508,740 GFF3075 GFF3076 - SSU rRNA pseudouridine(516) synthase (EC 5.4.99.19), at 508,889 to 509,638 GFF3076 GFF3077 - Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13), at 509,700 to 511,436 GFF3077 Position (kb) 507 508 509Strain fitness (log2 ratio) -2 -1 0 1 2 3at 507.016 kb on + strandat 507.016 kb on + strandat 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.251 kb on - strand, within GFF3074at 507.257 kb on - strand, within GFF3074at 507.316 kb on + strand, within GFF3074at 507.385 kb on + strand, within GFF3074at 507.487 kb on + strand, within GFF3074at 507.488 kb on - strand, within GFF3074at 507.499 kb on + strand, within GFF3074at 507.499 kb on + strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.500 kb on - strand, within GFF3074at 507.590 kb on + strand, within GFF3074at 507.590 kb on + strand, within GFF3074at 507.691 kb on + strand, within GFF3074at 507.691 kb on + strand, within GFF3074at 507.691 kb on + strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.692 kb on - strand, within GFF3074at 507.791 kb on + strandat 507.791 kb on + strandat 507.792 kb on - strandat 507.792 kb on - strandat 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.917 kb on + strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.918 kb on - strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.974 kb on + strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 507.975 kb on - strand, within GFF3075at 508.301 kb on + strand, within GFF3075at 508.302 kb on - strand, within GFF3075at 508.302 kb on - strand, within GFF3075at 508.302 kb on - strand, within GFF3075at 508.302 kb on - strand, within GFF3075at 508.397 kb on + strand, within GFF3075at 508.397 kb on + strand, within GFF3075at 508.398 kb on - strand, within GFF3075at 508.469 kb on + strand, within GFF3075at 508.469 kb on + strand, within GFF3075at 508.487 kb on + strand, within GFF3075at 508.487 kb on + strand, within GFF3075at 508.488 kb on - strand, within GFF3075at 508.488 kb on - strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.586 kb on + strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.587 kb on - strand, within GFF3075at 508.826 kb on - strandat 508.839 kb on + strandat 508.839 kb on + strandat 508.839 kb on + strandat 508.839 kb on + strandat 508.839 kb on + strandat 508.840 kb on - strandat 508.840 kb on - strandat 508.890 kb on + strandat 509.067 kb on + strand, within GFF3076at 509.067 kb on + strand, within GFF3076at 509.067 kb on + strand, within GFF3076at 509.067 kb on + strand, within GFF3076at 509.068 kb on - strand, within GFF3076at 509.068 kb on - strand, within GFF3076at 509.068 kb on - strand, within GFF3076at 509.221 kb on - strand, within GFF3076at 509.221 kb on - strand, within GFF3076at 509.221 kb on - strand, within GFF3076at 509.388 kb on + strand, within GFF3076at 509.388 kb on + strand, within GFF3076at 509.388 kb on + strand, within GFF3076at 509.388 kb on + strand, within GFF3076at 509.389 kb on - strand, within GFF3076at 509.389 kb on - strand, within GFF3076at 509.401 kb on - strand, within GFF3076at 509.401 kb on - strand, within GFF3076at 509.401 kb on - strand, within GFF3076at 509.454 kb on + strand, within GFF3076at 509.454 kb on + strand, within GFF3076at 509.455 kb on - strand, within GFF3076at 509.455 kb on - strand, within GFF3076at 509.455 kb on - strand, within GFF3076at 509.470 kb on - strand, within GFF3076at 509.496 kb on + strand, within GFF3076at 509.496 kb on + strand, within GFF3076at 509.497 kb on - strand, within GFF3076at 509.497 kb on - strand, within GFF3076at 509.497 kb on - strand, within GFF3076at 509.497 kb on - strand, within GFF3076at 509.497 kb on - strand, within GFF3076at 509.616 kb on + strandat 509.617 kb on - strandat 509.617 kb on - strandat 509.617 kb on - strandat 509.617 kb on - strandat 509.617 kb on - strandat 509.685 kb on + strandat 509.685 kb on + strandat 509.686 kb on - strandat 509.686 kb on - strandat 509.686 kb on - strandat 509.686 kb on - strandat 509.704 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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507,016 + +0.6
507,016 + -1.0
507,251 - GFF3074 0.33 -0.8
507,251 - GFF3074 0.33 -0.5
507,251 - GFF3074 0.33 -0.2
507,251 - GFF3074 0.33 +0.3
507,251 - GFF3074 0.33 +0.7
507,251 - GFF3074 0.33 -0.5
507,251 - GFF3074 0.33 -1.0
507,251 - GFF3074 0.33 -0.5
507,257 - GFF3074 0.34 -0.6
507,316 + GFF3074 0.42 +0.3
507,385 + GFF3074 0.51 -0.7
507,487 + GFF3074 0.63 -0.0
507,488 - GFF3074 0.64 +0.1
507,499 + GFF3074 0.65 -0.8
507,499 + GFF3074 0.65 -0.4
507,500 - GFF3074 0.65 +1.5
507,500 - GFF3074 0.65 +0.2
507,500 - GFF3074 0.65 -0.0
507,500 - GFF3074 0.65 +1.0
507,500 - GFF3074 0.65 +0.9
507,500 - GFF3074 0.65 -1.2
507,500 - GFF3074 0.65 +2.9
507,500 - GFF3074 0.65 -0.7
507,590 + GFF3074 0.77 -0.0
507,590 + GFF3074 0.77 -0.6
507,691 + GFF3074 0.89 +0.0
507,691 + GFF3074 0.89 -0.0
507,691 + GFF3074 0.89 -0.6
507,692 - GFF3074 0.90 +0.2
507,692 - GFF3074 0.90 -1.3
507,692 - GFF3074 0.90 -2.5
507,692 - GFF3074 0.90 -1.3
507,692 - GFF3074 0.90 -0.1
507,692 - GFF3074 0.90 +0.7
507,791 + -0.1
507,791 + -0.1
507,792 - -0.8
507,792 - -0.4
507,917 + GFF3075 0.13 -0.1
507,917 + GFF3075 0.13 -0.7
507,917 + GFF3075 0.13 -0.3
507,917 + GFF3075 0.13 -0.3
507,917 + GFF3075 0.13 +3.1
507,917 + GFF3075 0.13 +0.4
507,917 + GFF3075 0.13 +0.1
507,917 + GFF3075 0.13 -0.9
507,917 + GFF3075 0.13 -0.9
507,918 - GFF3075 0.13 -1.4
507,918 - GFF3075 0.13 -0.9
507,918 - GFF3075 0.13 +0.2
507,918 - GFF3075 0.13 +0.0
507,918 - GFF3075 0.13 +0.1
507,918 - GFF3075 0.13 -0.4
507,974 + GFF3075 0.19 +0.5
507,974 + GFF3075 0.19 -1.0
507,974 + GFF3075 0.19 -0.1
507,974 + GFF3075 0.19 -1.0
507,974 + GFF3075 0.19 -0.3
507,974 + GFF3075 0.19 -0.0
507,974 + GFF3075 0.19 +0.0
507,974 + GFF3075 0.19 -0.0
507,974 + GFF3075 0.19 -0.2
507,974 + GFF3075 0.19 -0.1
507,975 - GFF3075 0.19 +0.1
507,975 - GFF3075 0.19 -0.8
507,975 - GFF3075 0.19 +0.6
507,975 - GFF3075 0.19 -0.0
507,975 - GFF3075 0.19 -1.4
507,975 - GFF3075 0.19 -0.5
508,301 + GFF3075 0.54 -0.6
508,302 - GFF3075 0.54 -0.2
508,302 - GFF3075 0.54 -1.5
508,302 - GFF3075 0.54 +1.0
508,302 - GFF3075 0.54 +0.2
508,397 + GFF3075 0.64 +1.3
508,397 + GFF3075 0.64 -0.5
508,398 - GFF3075 0.64 -0.2
508,469 + GFF3075 0.71 -0.5
508,469 + GFF3075 0.71 -0.3
508,487 + GFF3075 0.73 -0.9
508,487 + GFF3075 0.73 -0.8
508,488 - GFF3075 0.73 -2.3
508,488 - GFF3075 0.73 +0.1
508,586 + GFF3075 0.84 +0.4
508,586 + GFF3075 0.84 -1.2
508,586 + GFF3075 0.84 -1.1
508,586 + GFF3075 0.84 -1.4
508,586 + GFF3075 0.84 -0.2
508,586 + GFF3075 0.84 +0.1
508,586 + GFF3075 0.84 -1.5
508,586 + GFF3075 0.84 +0.3
508,587 - GFF3075 0.84 -2.4
508,587 - GFF3075 0.84 -0.5
508,587 - GFF3075 0.84 -0.6
508,587 - GFF3075 0.84 -0.3
508,587 - GFF3075 0.84 -0.9
508,587 - GFF3075 0.84 -0.9
508,587 - GFF3075 0.84 +1.5
508,587 - GFF3075 0.84 +0.3
508,587 - GFF3075 0.84 -0.0
508,587 - GFF3075 0.84 -1.6
508,587 - GFF3075 0.84 -0.9
508,587 - GFF3075 0.84 +0.4
508,826 - -0.0
508,839 + -1.2
508,839 + -0.4
508,839 + -0.3
508,839 + -0.0
508,839 + +0.1
508,840 - -1.1
508,840 - -0.6
508,890 + -2.2
509,067 + GFF3076 0.24 -1.5
509,067 + GFF3076 0.24 +0.2
509,067 + GFF3076 0.24 -0.0
509,067 + GFF3076 0.24 +0.3
509,068 - GFF3076 0.24 -1.3
509,068 - GFF3076 0.24 -0.0
509,068 - GFF3076 0.24 -1.0
509,221 - GFF3076 0.44 -1.0
509,221 - GFF3076 0.44 -0.8
509,221 - GFF3076 0.44 +0.1
509,388 + GFF3076 0.67 +0.3
509,388 + GFF3076 0.67 -0.6
509,388 + GFF3076 0.67 -2.4
509,388 + GFF3076 0.67 +0.2
509,389 - GFF3076 0.67 -0.3
509,389 - GFF3076 0.67 +0.1
509,401 - GFF3076 0.68 -0.0
509,401 - GFF3076 0.68 +0.9
509,401 - GFF3076 0.68 -0.6
509,454 + GFF3076 0.75 -0.3
509,454 + GFF3076 0.75 +0.1
509,455 - GFF3076 0.75 +0.1
509,455 - GFF3076 0.75 -0.2
509,455 - GFF3076 0.75 -0.2
509,470 - GFF3076 0.77 -1.5
509,496 + GFF3076 0.81 +0.1
509,496 + GFF3076 0.81 -0.0
509,497 - GFF3076 0.81 -0.3
509,497 - GFF3076 0.81 -0.4
509,497 - GFF3076 0.81 +0.1
509,497 - GFF3076 0.81 -0.0
509,497 - GFF3076 0.81 -0.5
509,616 + -0.4
509,617 - -0.0
509,617 - +0.3
509,617 - +0.1
509,617 - -0.3
509,617 - -0.1
509,685 + -0.6
509,685 + -0.4
509,686 - -0.4
509,686 - -0.8
509,686 - +1.1
509,686 - -0.5
509,704 + +0.0

Or see this region's nucleotide sequence