Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3042 and GFF3043 are separated by 4 nucleotides GFF3043 and GFF3044 are separated by 69 nucleotides
GFF3042 - hypothetical protein, at 476,143 to 476,913
GFF3042
GFF3043 - Response regulator BaeR, at 476,918 to 477,607
GFF3043
GFF3044 - LPS-assembly protein LptD @ Organic solvent tolerance protein precursor, at 477,677 to 480,034
GFF3044
Position (kb)
476
477
478 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 475.929 kb on + strand at 475.930 kb on - strand at 475.930 kb on - strand at 475.964 kb on + strand at 476.140 kb on + strand at 476.140 kb on + strand at 476.141 kb on - strand at 476.141 kb on - strand at 476.141 kb on - strand at 476.141 kb on - strand at 476.141 kb on - strand at 476.161 kb on - strand at 476.365 kb on + strand, within GFF3042 at 476.365 kb on + strand, within GFF3042 at 476.366 kb on - strand, within GFF3042 at 476.428 kb on + strand, within GFF3042 at 476.429 kb on - strand, within GFF3042 at 476.617 kb on + strand, within GFF3042 at 476.617 kb on + strand, within GFF3042 at 476.698 kb on + strand, within GFF3042 at 476.699 kb on - strand, within GFF3042 at 476.699 kb on - strand, within GFF3042 at 476.744 kb on - strand, within GFF3042 at 477.210 kb on - strand, within GFF3043 at 477.210 kb on - strand, within GFF3043 at 477.210 kb on - strand, within GFF3043 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.152 kb on - strand, within GFF3044 at 478.386 kb on - strand, within GFF3044 at 478.397 kb on + strand, within GFF3044 at 478.398 kb on - strand, within GFF3044 at 478.401 kb on - strand, within GFF3044 at 478.401 kb on - strand, within GFF3044 at 478.502 kb on + strand, within GFF3044 at 478.503 kb on - strand, within GFF3044
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 475,929 + -0.1 475,930 - -0.2 475,930 - -0.6 475,964 + -0.5 476,140 + +0.0 476,140 + -0.6 476,141 - -0.3 476,141 - +0.6 476,141 - -0.8 476,141 - -0.2 476,141 - -0.0 476,161 - -0.0 476,365 + GFF3042 0.29 +0.2 476,365 + GFF3042 0.29 -2.3 476,366 - GFF3042 0.29 +0.6 476,428 + GFF3042 0.37 -0.3 476,429 - GFF3042 0.37 +1.1 476,617 + GFF3042 0.61 -3.2 476,617 + GFF3042 0.61 -0.6 476,698 + GFF3042 0.72 -0.4 476,699 - GFF3042 0.72 +2.0 476,699 - GFF3042 0.72 +0.8 476,744 - GFF3042 0.78 +0.2 477,210 - GFF3043 0.42 -0.9 477,210 - GFF3043 0.42 +0.8 477,210 - GFF3043 0.42 -1.3 478,152 - GFF3044 0.20 +0.6 478,152 - GFF3044 0.20 -1.4 478,152 - GFF3044 0.20 -0.5 478,152 - GFF3044 0.20 +0.0 478,386 - GFF3044 0.30 -0.3 478,397 + GFF3044 0.31 -0.0 478,398 - GFF3044 0.31 -0.2 478,401 - GFF3044 0.31 +0.8 478,401 - GFF3044 0.31 -0.9 478,502 + GFF3044 0.35 -1.2 478,503 - GFF3044 0.35 +1.3
Or see this region's nucleotide sequence