Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3006 and GFF3007 are separated by 165 nucleotides GFF3007 and GFF3008 overlap by 14 nucleotides GFF3008 and GFF3009 are separated by 16 nucleotides GFF3009 and GFF3010 are separated by 395 nucleotides GFF3010 and GFF3011 overlap by 11 nucleotides
GFF3006 - hypothetical protein, at 434,352 to 435,473
GFF3006
GFF3007 - hypothetical protein, at 435,639 to 435,878
GFF3007
GFF3008 - hypothetical protein, at 435,865 to 436,215
GFF3008
GFF3009 - NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 436,232 to 437,575
GFF3009
GFF3010 - no description, at 437,971 to 438,114
GFF3010
GFF3011 - T6SS component TssB (ImpB/VipA), at 438,104 to 438,679
GFF3011
Position (kb)
436
437
438 Strain fitness (log2 ratio)
-1
0
1
2 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.384 kb on + strand at 435.539 kb on + strand at 435.540 kb on - strand at 435.574 kb on - strand at 435.583 kb on + strand at 435.583 kb on + strand at 435.583 kb on + strand at 435.583 kb on + strand at 435.584 kb on - strand at 435.584 kb on - strand at 435.584 kb on - strand at 435.584 kb on - strand at 435.584 kb on - strand at 435.584 kb on - strand at 435.649 kb on - strand at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.684 kb on + strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.685 kb on - strand, within GFF3007 at 435.746 kb on - strand, within GFF3007 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.986 kb on + strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 435.987 kb on - strand, within GFF3008 at 436.092 kb on - strand, within GFF3008 at 436.092 kb on - strand, within GFF3008 at 436.092 kb on - strand, within GFF3008 at 436.129 kb on + strand, within GFF3008 at 436.130 kb on + strand, within GFF3008 at 436.130 kb on + strand, within GFF3008 at 436.130 kb on + strand, within GFF3008 at 436.131 kb on - strand, within GFF3008 at 436.131 kb on - strand, within GFF3008 at 436.228 kb on - strand at 436.302 kb on + strand at 436.302 kb on + strand at 436.302 kb on + strand at 436.302 kb on + strand at 436.302 kb on + strand at 436.302 kb on + strand at 436.482 kb on + strand, within GFF3009 at 436.482 kb on + strand, within GFF3009 at 436.482 kb on + strand, within GFF3009 at 436.483 kb on - strand, within GFF3009 at 436.483 kb on - strand, within GFF3009 at 436.483 kb on - strand, within GFF3009 at 436.483 kb on - strand, within GFF3009 at 436.692 kb on + strand, within GFF3009 at 436.693 kb on - strand, within GFF3009 at 436.710 kb on + strand, within GFF3009 at 436.710 kb on + strand, within GFF3009 at 436.710 kb on + strand, within GFF3009 at 436.710 kb on + strand, within GFF3009 at 436.711 kb on - strand, within GFF3009 at 436.711 kb on - strand, within GFF3009 at 436.711 kb on - strand, within GFF3009 at 436.780 kb on + strand, within GFF3009 at 436.781 kb on - strand, within GFF3009 at 436.842 kb on + strand, within GFF3009 at 436.842 kb on + strand, within GFF3009 at 436.842 kb on + strand, within GFF3009 at 436.843 kb on - strand, within GFF3009 at 436.843 kb on - strand, within GFF3009 at 436.843 kb on - strand, within GFF3009 at 436.843 kb on - strand, within GFF3009 at 436.929 kb on + strand, within GFF3009 at 436.929 kb on + strand, within GFF3009 at 436.929 kb on + strand, within GFF3009 at 436.930 kb on - strand, within GFF3009 at 436.930 kb on - strand, within GFF3009 at 437.307 kb on + strand, within GFF3009 at 437.307 kb on + strand, within GFF3009 at 437.307 kb on + strand, within GFF3009 at 437.308 kb on - strand, within GFF3009 at 437.308 kb on - strand, within GFF3009 at 437.308 kb on - strand, within GFF3009 at 437.445 kb on + strand at 437.445 kb on + strand at 437.466 kb on + strand at 437.466 kb on + strand at 437.466 kb on + strand at 437.467 kb on - strand at 437.467 kb on - strand at 437.467 kb on - strand at 437.467 kb on - strand at 437.467 kb on - strand at 437.508 kb on + strand at 437.508 kb on + strand at 437.508 kb on + strand at 437.508 kb on + strand at 437.509 kb on - strand at 437.509 kb on - strand at 437.509 kb on - strand at 437.509 kb on - strand at 437.605 kb on + strand at 437.605 kb on + strand at 437.605 kb on + strand at 437.606 kb on - strand at 437.768 kb on + strand at 437.831 kb on + strand at 437.831 kb on + strand at 437.831 kb on + strand at 437.846 kb on + strand at 437.846 kb on + strand at 437.846 kb on + strand at 437.847 kb on - strand at 437.847 kb on - strand at 437.847 kb on - strand at 437.847 kb on - strand at 437.847 kb on - strand at 437.868 kb on + strand at 437.868 kb on + strand at 437.868 kb on + strand at 437.868 kb on + strand at 437.868 kb on + strand at 437.868 kb on + strand at 437.869 kb on - strand at 437.869 kb on - strand at 437.869 kb on - strand at 437.869 kb on - strand at 437.869 kb on - strand at 437.870 kb on + strand at 437.870 kb on + strand at 437.871 kb on - strand at 437.871 kb on - strand at 437.871 kb on - strand at 437.871 kb on - strand at 437.871 kb on - strand at 437.871 kb on - strand at 437.889 kb on + strand at 437.889 kb on + strand at 437.890 kb on - strand at 437.890 kb on - strand at 437.890 kb on - strand at 437.890 kb on - strand at 437.890 kb on - strand at 437.903 kb on + strand at 437.903 kb on + strand at 437.955 kb on + strand at 437.955 kb on + strand at 437.955 kb on + strand at 437.956 kb on - strand at 437.956 kb on - strand at 437.956 kb on - strand at 437.958 kb on + strand at 437.974 kb on + strand at 437.974 kb on + strand at 437.975 kb on - strand at 438.112 kb on + strand at 438.112 kb on + strand at 438.112 kb on + strand at 438.112 kb on + strand at 438.113 kb on - strand at 438.113 kb on - strand at 438.113 kb on - strand at 438.113 kb on - strand at 438.113 kb on - strand at 438.113 kb on - strand at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.164 kb on + strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011 at 438.165 kb on - strand, within GFF3011
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 435,297 + GFF3006 0.84 +1.1 435,297 + GFF3006 0.84 -0.8 435,297 + GFF3006 0.84 -0.3 435,297 + GFF3006 0.84 +0.0 435,298 - GFF3006 0.84 +0.2 435,298 - GFF3006 0.84 +2.1 435,298 - GFF3006 0.84 +1.1 435,384 + +0.2 435,539 + -0.1 435,540 - -0.2 435,574 - -0.8 435,583 + +0.3 435,583 + -1.6 435,583 + -0.1 435,583 + -0.0 435,584 - +0.0 435,584 - +0.1 435,584 - -1.5 435,584 - -0.8 435,584 - -0.2 435,584 - +0.0 435,649 - -0.1 435,684 + GFF3007 0.19 +0.9 435,684 + GFF3007 0.19 -0.5 435,684 + GFF3007 0.19 -0.8 435,684 + GFF3007 0.19 +0.3 435,684 + GFF3007 0.19 +0.1 435,684 + GFF3007 0.19 +0.3 435,684 + GFF3007 0.19 -1.2 435,684 + GFF3007 0.19 -0.8 435,684 + GFF3007 0.19 +0.1 435,684 + GFF3007 0.19 +0.3 435,685 - GFF3007 0.19 +0.9 435,685 - GFF3007 0.19 +0.2 435,685 - GFF3007 0.19 +0.8 435,685 - GFF3007 0.19 -0.1 435,685 - GFF3007 0.19 -0.2 435,685 - GFF3007 0.19 -1.2 435,685 - GFF3007 0.19 +0.7 435,685 - GFF3007 0.19 +0.1 435,746 - GFF3007 0.45 +0.8 435,986 + GFF3008 0.34 -0.2 435,986 + GFF3008 0.34 -0.1 435,986 + GFF3008 0.34 -0.6 435,986 + GFF3008 0.34 -1.3 435,986 + GFF3008 0.34 -0.2 435,986 + GFF3008 0.34 -0.7 435,986 + GFF3008 0.34 +1.5 435,986 + GFF3008 0.34 -0.1 435,986 + GFF3008 0.34 -0.1 435,986 + GFF3008 0.34 -0.8 435,986 + GFF3008 0.34 -1.3 435,986 + GFF3008 0.34 +0.0 435,987 - GFF3008 0.35 +0.1 435,987 - GFF3008 0.35 -0.6 435,987 - GFF3008 0.35 -0.4 435,987 - GFF3008 0.35 -1.1 435,987 - GFF3008 0.35 +0.2 435,987 - GFF3008 0.35 -1.6 435,987 - GFF3008 0.35 -0.4 435,987 - GFF3008 0.35 -1.3 436,092 - GFF3008 0.65 +1.2 436,092 - GFF3008 0.65 -0.3 436,092 - GFF3008 0.65 +0.5 436,129 + GFF3008 0.75 -0.0 436,130 + GFF3008 0.75 -0.1 436,130 + GFF3008 0.75 +0.6 436,130 + GFF3008 0.75 -0.5 436,131 - GFF3008 0.76 -0.5 436,131 - GFF3008 0.76 +0.2 436,228 - -0.1 436,302 + +0.2 436,302 + +0.7 436,302 + -1.3 436,302 + +0.7 436,302 + -0.5 436,302 + -0.1 436,482 + GFF3009 0.19 -0.5 436,482 + GFF3009 0.19 -0.4 436,482 + GFF3009 0.19 -0.3 436,483 - GFF3009 0.19 +0.4 436,483 - GFF3009 0.19 +0.9 436,483 - GFF3009 0.19 -0.0 436,483 - GFF3009 0.19 -0.3 436,692 + GFF3009 0.34 +0.2 436,693 - GFF3009 0.34 -0.9 436,710 + GFF3009 0.36 +0.8 436,710 + GFF3009 0.36 +0.7 436,710 + GFF3009 0.36 +0.5 436,710 + GFF3009 0.36 +0.2 436,711 - GFF3009 0.36 +1.5 436,711 - GFF3009 0.36 -0.3 436,711 - GFF3009 0.36 +0.2 436,780 + GFF3009 0.41 -0.6 436,781 - GFF3009 0.41 -0.5 436,842 + GFF3009 0.45 +0.4 436,842 + GFF3009 0.45 +0.3 436,842 + GFF3009 0.45 +1.0 436,843 - GFF3009 0.45 -0.6 436,843 - GFF3009 0.45 -1.0 436,843 - GFF3009 0.45 +0.1 436,843 - GFF3009 0.45 -0.7 436,929 + GFF3009 0.52 -0.5 436,929 + GFF3009 0.52 -0.3 436,929 + GFF3009 0.52 -0.7 436,930 - GFF3009 0.52 +1.4 436,930 - GFF3009 0.52 -0.0 437,307 + GFF3009 0.80 -0.5 437,307 + GFF3009 0.80 +0.6 437,307 + GFF3009 0.80 -1.3 437,308 - GFF3009 0.80 +0.4 437,308 - GFF3009 0.80 -0.8 437,308 - GFF3009 0.80 +1.3 437,445 + -0.7 437,445 + -0.8 437,466 + +1.7 437,466 + -1.1 437,466 + -0.8 437,467 - -0.7 437,467 - +2.5 437,467 - +0.4 437,467 - -0.4 437,467 - -0.4 437,508 + +0.1 437,508 + +0.3 437,508 + -0.8 437,508 + -0.2 437,509 - -0.5 437,509 - -0.6 437,509 - +0.4 437,509 - -0.5 437,605 + -1.0 437,605 + -0.0 437,605 + +1.0 437,606 - +0.8 437,768 + -0.1 437,831 + -0.4 437,831 + -1.1 437,831 + +0.2 437,846 + -0.7 437,846 + -0.5 437,846 + -0.0 437,847 - +0.1 437,847 - -0.2 437,847 - +0.2 437,847 - -0.2 437,847 - -0.7 437,868 + -0.4 437,868 + -0.9 437,868 + -0.1 437,868 + -0.6 437,868 + +0.5 437,868 + -0.0 437,869 - -1.3 437,869 - +0.3 437,869 - -1.1 437,869 - +0.1 437,869 - -0.6 437,870 + -0.5 437,870 + -0.4 437,871 - -0.3 437,871 - -0.9 437,871 - +0.0 437,871 - -0.5 437,871 - +1.5 437,871 - -1.5 437,889 + +1.1 437,889 + -1.5 437,890 - -0.4 437,890 - +0.8 437,890 - -1.5 437,890 - -0.5 437,890 - -0.3 437,903 + +0.1 437,903 + -0.0 437,955 + -0.2 437,955 + -0.8 437,955 + -0.9 437,956 - +0.6 437,956 - -0.5 437,956 - +0.2 437,958 + +0.1 437,974 + +0.9 437,974 + -0.1 437,975 - -0.0 438,112 + -1.4 438,112 + -1.1 438,112 + -0.3 438,112 + -0.4 438,113 - -0.6 438,113 - -0.3 438,113 - -0.7 438,113 - +0.3 438,113 - -0.6 438,113 - -0.5 438,164 + GFF3011 0.10 +0.1 438,164 + GFF3011 0.10 -0.4 438,164 + GFF3011 0.10 -1.5 438,164 + GFF3011 0.10 -0.4 438,164 + GFF3011 0.10 +0.1 438,164 + GFF3011 0.10 +0.2 438,164 + GFF3011 0.10 +0.1 438,164 + GFF3011 0.10 -1.6 438,164 + GFF3011 0.10 +0.0 438,164 + GFF3011 0.10 -0.6 438,164 + GFF3011 0.10 +2.3 438,164 + GFF3011 0.10 -1.0 438,164 + GFF3011 0.10 -0.3 438,164 + GFF3011 0.10 -0.5 438,164 + GFF3011 0.10 -0.3 438,164 + GFF3011 0.10 -0.1 438,164 + GFF3011 0.10 -0.3 438,164 + GFF3011 0.10 -0.6 438,164 + GFF3011 0.10 -0.0 438,164 + GFF3011 0.10 -0.3 438,164 + GFF3011 0.10 +0.2 438,165 - GFF3011 0.11 -0.0 438,165 - GFF3011 0.11 +0.7 438,165 - GFF3011 0.11 +0.1 438,165 - GFF3011 0.11 +0.3 438,165 - GFF3011 0.11 -0.1 438,165 - GFF3011 0.11 -0.6 438,165 - GFF3011 0.11 -0.3 438,165 - GFF3011 0.11 -0.1 438,165 - GFF3011 0.11 -0.4 438,165 - GFF3011 0.11 -1.5 438,165 - GFF3011 0.11 +0.2 438,165 - GFF3011 0.11 -0.0 438,165 - GFF3011 0.11 +0.4 438,165 - GFF3011 0.11 -0.8 438,165 - GFF3011 0.11 -0.4
Or see this region's nucleotide sequence