Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2970 and GFF2971 are separated by 130 nucleotides GFF2971 and GFF2972 overlap by 50 nucleotides
GFF2970 - BUG/TctC family periplasmic protein, at 402,208 to 403,194
GFF2970
GFF2971 - Transcriptional regulator, LysR family, at 403,325 to 404,290
GFF2971
GFF2972 - Radical SAM domain protein, at 404,241 to 405,335
GFF2972
Position (kb) 403
404
405 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.375 kb on + strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.376 kb on - strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.471 kb on + strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.472 kb on - strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.560 kb on + strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.561 kb on - strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.671 kb on + strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.672 kb on - strand, within GFF2970 at 402.832 kb on + strand, within GFF2970 at 402.833 kb on - strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.854 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.869 kb on + strand, within GFF2970 at 402.870 kb on - strand, within GFF2970 at 402.917 kb on + strand, within GFF2970 at 403.101 kb on - strand at 403.101 kb on - strand at 403.262 kb on + strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.263 kb on - strand at 403.274 kb on + strand at 403.276 kb on + strand at 403.289 kb on - strand at 403.312 kb on - strand at 403.312 kb on - strand at 403.402 kb on + strand at 403.402 kb on + strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.403 kb on - strand at 403.582 kb on - strand, within GFF2971 at 404.053 kb on - strand, within GFF2971 at 404.072 kb on + strand, within GFF2971 at 404.073 kb on - strand, within GFF2971 at 404.138 kb on - strand, within GFF2971 at 404.150 kb on + strand, within GFF2971 at 404.242 kb on + strand at 404.242 kb on + strand at 404.242 kb on + strand at 404.242 kb on + strand at 404.242 kb on + strand at 404.243 kb on - strand at 404.243 kb on - strand at 404.243 kb on - strand at 404.243 kb on - strand at 404.243 kb on - strand at 404.410 kb on + strand, within GFF2972 at 404.410 kb on + strand, within GFF2972 at 404.411 kb on - strand, within GFF2972 at 404.411 kb on - strand, within GFF2972 at 404.411 kb on - strand, within GFF2972 at 404.470 kb on + strand, within GFF2972 at 404.533 kb on + strand, within GFF2972 at 404.533 kb on + strand, within GFF2972 at 404.533 kb on + strand, within GFF2972 at 404.533 kb on + strand, within GFF2972 at 404.534 kb on - strand, within GFF2972 at 404.534 kb on - strand, within GFF2972 at 404.534 kb on - strand, within GFF2972 at 404.534 kb on - strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.743 kb on + strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.744 kb on - strand, within GFF2972 at 404.899 kb on + strand, within GFF2972 at 404.900 kb on - strand, within GFF2972 at 404.900 kb on - strand, within GFF2972 at 404.900 kb on - strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.929 kb on + strand, within GFF2972 at 404.930 kb on - strand, within GFF2972 at 404.930 kb on - strand, within GFF2972 at 404.930 kb on - strand, within GFF2972 at 404.930 kb on - strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.941 kb on + strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 404.942 kb on - strand, within GFF2972 at 405.037 kb on + strand, within GFF2972 at 405.037 kb on + strand, within GFF2972 at 405.037 kb on + strand, within GFF2972 at 405.037 kb on + strand, within GFF2972 at 405.037 kb on + strand, within GFF2972 at 405.038 kb on - strand, within GFF2972 at 405.038 kb on - strand, within GFF2972 at 405.038 kb on - strand, within GFF2972 at 405.038 kb on - strand, within GFF2972 at 405.038 kb on - strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.064 kb on + strand, within GFF2972 at 405.065 kb on - strand, within GFF2972 at 405.065 kb on - strand, within GFF2972 at 405.065 kb on - strand, within GFF2972 at 405.065 kb on - strand, within GFF2972 at 405.115 kb on + strand, within GFF2972 at 405.115 kb on + strand, within GFF2972 at 405.115 kb on + strand, within GFF2972 at 405.116 kb on - strand, within GFF2972 at 405.116 kb on - strand, within GFF2972 at 405.116 kb on - strand, within GFF2972 at 405.116 kb on - strand, within GFF2972
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 402,375 + GFF2970 0.17 -0.3 402,375 + GFF2970 0.17 +0.2 402,375 + GFF2970 0.17 -0.1 402,375 + GFF2970 0.17 +0.8 402,376 - GFF2970 0.17 -0.5 402,376 - GFF2970 0.17 +0.3 402,376 - GFF2970 0.17 +1.5 402,376 - GFF2970 0.17 +1.2 402,471 + GFF2970 0.27 -1.2 402,471 + GFF2970 0.27 +1.4 402,471 + GFF2970 0.27 +0.1 402,471 + GFF2970 0.27 +0.9 402,472 - GFF2970 0.27 -1.1 402,472 - GFF2970 0.27 -0.4 402,472 - GFF2970 0.27 -1.6 402,472 - GFF2970 0.27 +0.2 402,560 + GFF2970 0.36 -0.1 402,560 + GFF2970 0.36 +2.1 402,560 + GFF2970 0.36 +0.1 402,560 + GFF2970 0.36 -0.4 402,560 + GFF2970 0.36 -0.3 402,561 - GFF2970 0.36 -1.3 402,561 - GFF2970 0.36 +0.4 402,561 - GFF2970 0.36 +0.0 402,561 - GFF2970 0.36 -0.6 402,671 + GFF2970 0.47 -0.1 402,671 + GFF2970 0.47 -0.7 402,672 - GFF2970 0.47 -0.5 402,672 - GFF2970 0.47 -0.6 402,832 + GFF2970 0.63 -0.5 402,833 - GFF2970 0.63 +1.0 402,854 + GFF2970 0.65 -0.5 402,854 + GFF2970 0.65 -0.1 402,869 + GFF2970 0.67 -0.2 402,869 + GFF2970 0.67 -0.5 402,869 + GFF2970 0.67 -1.1 402,870 - GFF2970 0.67 -0.1 402,917 + GFF2970 0.72 +0.2 403,101 - +0.2 403,101 - -0.1 403,262 + -0.8 403,263 - -0.5 403,263 - -0.2 403,263 - +1.5 403,274 + +1.0 403,276 + -0.2 403,289 - -1.9 403,312 - -0.7 403,312 - -0.9 403,402 + -3.1 403,402 + -0.3 403,403 - -1.5 403,403 - -0.6 403,403 - +0.1 403,582 - GFF2971 0.27 -0.8 404,053 - GFF2971 0.75 -1.5 404,072 + GFF2971 0.77 +0.9 404,073 - GFF2971 0.77 -2.2 404,138 - GFF2971 0.84 -1.1 404,150 + GFF2971 0.85 +0.1 404,242 + +0.6 404,242 + -0.4 404,242 + +0.4 404,242 + -0.5 404,242 + -0.5 404,243 - -2.0 404,243 - +1.4 404,243 - -0.4 404,243 - +0.3 404,243 - -0.5 404,410 + GFF2972 0.15 -0.2 404,410 + GFF2972 0.15 -0.6 404,411 - GFF2972 0.16 +0.2 404,411 - GFF2972 0.16 +0.1 404,411 - GFF2972 0.16 +0.1 404,470 + GFF2972 0.21 +1.3 404,533 + GFF2972 0.27 +0.2 404,533 + GFF2972 0.27 -0.2 404,533 + GFF2972 0.27 +1.2 404,533 + GFF2972 0.27 -0.5 404,534 - GFF2972 0.27 -0.5 404,534 - GFF2972 0.27 +0.9 404,534 - GFF2972 0.27 +1.0 404,534 - GFF2972 0.27 -0.5 404,743 + GFF2972 0.46 +0.3 404,743 + GFF2972 0.46 -0.6 404,743 + GFF2972 0.46 -0.6 404,743 + GFF2972 0.46 +0.2 404,743 + GFF2972 0.46 +0.7 404,743 + GFF2972 0.46 +0.5 404,743 + GFF2972 0.46 +0.9 404,743 + GFF2972 0.46 -1.5 404,743 + GFF2972 0.46 +3.1 404,744 - GFF2972 0.46 -0.1 404,744 - GFF2972 0.46 +0.5 404,744 - GFF2972 0.46 -0.6 404,744 - GFF2972 0.46 -0.3 404,744 - GFF2972 0.46 -0.2 404,744 - GFF2972 0.46 -0.3 404,899 + GFF2972 0.60 +0.8 404,900 - GFF2972 0.60 -0.6 404,900 - GFF2972 0.60 -1.0 404,900 - GFF2972 0.60 -0.5 404,929 + GFF2972 0.63 +0.0 404,929 + GFF2972 0.63 -0.1 404,929 + GFF2972 0.63 +0.5 404,929 + GFF2972 0.63 -0.8 404,929 + GFF2972 0.63 +0.4 404,929 + GFF2972 0.63 +0.1 404,929 + GFF2972 0.63 +1.3 404,930 - GFF2972 0.63 -0.8 404,930 - GFF2972 0.63 +1.5 404,930 - GFF2972 0.63 +1.0 404,930 - GFF2972 0.63 -0.4 404,941 + GFF2972 0.64 -0.3 404,941 + GFF2972 0.64 +0.1 404,941 + GFF2972 0.64 +0.1 404,941 + GFF2972 0.64 -0.2 404,941 + GFF2972 0.64 +0.5 404,941 + GFF2972 0.64 -0.9 404,941 + GFF2972 0.64 +0.0 404,942 - GFF2972 0.64 +0.2 404,942 - GFF2972 0.64 -0.3 404,942 - GFF2972 0.64 -1.3 404,942 - GFF2972 0.64 -0.6 404,942 - GFF2972 0.64 -2.6 404,942 - GFF2972 0.64 -0.4 404,942 - GFF2972 0.64 -0.2 404,942 - GFF2972 0.64 -0.5 405,037 + GFF2972 0.73 -1.8 405,037 + GFF2972 0.73 -1.9 405,037 + GFF2972 0.73 -0.3 405,037 + GFF2972 0.73 -0.8 405,037 + GFF2972 0.73 +0.0 405,038 - GFF2972 0.73 -1.2 405,038 - GFF2972 0.73 +0.2 405,038 - GFF2972 0.73 -0.5 405,038 - GFF2972 0.73 +0.4 405,038 - GFF2972 0.73 +1.0 405,064 + GFF2972 0.75 -0.8 405,064 + GFF2972 0.75 -0.2 405,064 + GFF2972 0.75 +0.7 405,064 + GFF2972 0.75 +0.3 405,064 + GFF2972 0.75 -0.7 405,064 + GFF2972 0.75 -0.2 405,064 + GFF2972 0.75 -0.5 405,064 + GFF2972 0.75 +0.2 405,065 - GFF2972 0.75 +0.7 405,065 - GFF2972 0.75 -0.6 405,065 - GFF2972 0.75 -0.3 405,065 - GFF2972 0.75 +0.1 405,115 + GFF2972 0.80 -0.4 405,115 + GFF2972 0.80 -0.3 405,115 + GFF2972 0.80 -1.1 405,116 - GFF2972 0.80 -1.0 405,116 - GFF2972 0.80 -0.8 405,116 - GFF2972 0.80 -0.3 405,116 - GFF2972 0.80 -0.1
Or see this region's nucleotide sequence