Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2818 and GFF2819 are separated by 177 nucleotides GFF2819 and GFF2820 are separated by 49 nucleotides GFF2820 and GFF2821 overlap by 4 nucleotides
GFF2818 - Phosphoenolpyruvate synthase (EC 2.7.9.2), at 243,453 to 245,846
GFF2818
GFF2819 - Phosphoenolpyruvate synthase regulatory protein, at 246,024 to 246,851
GFF2819
GFF2820 - ABC-type hemin transport system, ATPase component, at 246,901 to 247,707
GFF2820
GFF2821 - Hemin ABC transporter, permease protein, at 247,704 to 248,738
GFF2821
Position (kb)
246
247 Strain fitness (log2 ratio)
-2
-1
0
1 at 245.122 kb on + strand, within GFF2818 at 245.122 kb on + strand, within GFF2818 at 245.122 kb on + strand, within GFF2818 at 245.122 kb on + strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.123 kb on - strand, within GFF2818 at 245.173 kb on + strand, within GFF2818 at 245.173 kb on + strand, within GFF2818 at 245.173 kb on + strand, within GFF2818 at 245.174 kb on - strand, within GFF2818 at 245.174 kb on - strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.183 kb on + strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.184 kb on - strand, within GFF2818 at 245.323 kb on + strand, within GFF2818 at 245.323 kb on + strand, within GFF2818 at 245.324 kb on - strand, within GFF2818 at 245.324 kb on - strand, within GFF2818 at 245.324 kb on - strand, within GFF2818 at 245.344 kb on + strand, within GFF2818 at 245.344 kb on + strand, within GFF2818 at 245.344 kb on + strand, within GFF2818 at 245.344 kb on + strand, within GFF2818 at 245.344 kb on + strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.345 kb on - strand, within GFF2818 at 245.811 kb on + strand at 245.812 kb on - strand at 245.812 kb on - strand at 245.812 kb on - strand at 245.812 kb on - strand at 245.812 kb on - strand at 245.984 kb on + strand at 245.985 kb on - strand at 245.985 kb on - strand at 245.985 kb on - strand at 245.985 kb on - strand at 245.985 kb on - strand at 246.329 kb on - strand, within GFF2819 at 246.393 kb on + strand, within GFF2819 at 246.393 kb on + strand, within GFF2819 at 246.393 kb on + strand, within GFF2819 at 246.393 kb on + strand, within GFF2819 at 246.393 kb on + strand, within GFF2819 at 246.394 kb on - strand, within GFF2819 at 246.522 kb on + strand, within GFF2819 at 246.522 kb on + strand, within GFF2819 at 246.523 kb on - strand, within GFF2819 at 246.558 kb on + strand, within GFF2819 at 246.846 kb on + strand at 246.846 kb on + strand at 246.847 kb on - strand at 246.914 kb on + strand at 246.914 kb on + strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.059 kb on - strand, within GFF2820 at 247.248 kb on + strand, within GFF2820 at 247.249 kb on - strand, within GFF2820 at 247.249 kb on - strand, within GFF2820 at 247.379 kb on + strand, within GFF2820 at 247.380 kb on - strand, within GFF2820 at 247.644 kb on + strand at 247.644 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.817 kb on + strand, within GFF2821
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 245,122 + GFF2818 0.70 -2.2 245,122 + GFF2818 0.70 +0.3 245,122 + GFF2818 0.70 +1.5 245,122 + GFF2818 0.70 -1.6 245,123 - GFF2818 0.70 -1.7 245,123 - GFF2818 0.70 -0.7 245,123 - GFF2818 0.70 -0.9 245,123 - GFF2818 0.70 -2.5 245,123 - GFF2818 0.70 +0.1 245,123 - GFF2818 0.70 -0.8 245,123 - GFF2818 0.70 -0.9 245,123 - GFF2818 0.70 -1.8 245,123 - GFF2818 0.70 -0.3 245,123 - GFF2818 0.70 +0.2 245,123 - GFF2818 0.70 -0.4 245,123 - GFF2818 0.70 -0.4 245,123 - GFF2818 0.70 -0.1 245,123 - GFF2818 0.70 +0.3 245,123 - GFF2818 0.70 -1.7 245,173 + GFF2818 0.72 -2.2 245,173 + GFF2818 0.72 -0.5 245,173 + GFF2818 0.72 -0.4 245,174 - GFF2818 0.72 -0.3 245,174 - GFF2818 0.72 -0.9 245,183 + GFF2818 0.72 -0.6 245,183 + GFF2818 0.72 -1.6 245,183 + GFF2818 0.72 -1.3 245,183 + GFF2818 0.72 -0.6 245,183 + GFF2818 0.72 -1.7 245,183 + GFF2818 0.72 -2.2 245,183 + GFF2818 0.72 +0.2 245,183 + GFF2818 0.72 -0.6 245,183 + GFF2818 0.72 -0.6 245,183 + GFF2818 0.72 -2.3 245,183 + GFF2818 0.72 +0.1 245,183 + GFF2818 0.72 -1.0 245,183 + GFF2818 0.72 -0.6 245,184 - GFF2818 0.72 -1.2 245,184 - GFF2818 0.72 -0.3 245,184 - GFF2818 0.72 -1.8 245,184 - GFF2818 0.72 +0.8 245,184 - GFF2818 0.72 -0.6 245,184 - GFF2818 0.72 +0.0 245,184 - GFF2818 0.72 -0.0 245,323 + GFF2818 0.78 -0.3 245,323 + GFF2818 0.78 +1.1 245,324 - GFF2818 0.78 -0.0 245,324 - GFF2818 0.78 -0.7 245,324 - GFF2818 0.78 +0.2 245,344 + GFF2818 0.79 -0.3 245,344 + GFF2818 0.79 -0.3 245,344 + GFF2818 0.79 -0.2 245,344 + GFF2818 0.79 -2.5 245,344 + GFF2818 0.79 -1.5 245,345 - GFF2818 0.79 +0.2 245,345 - GFF2818 0.79 -0.7 245,345 - GFF2818 0.79 -0.8 245,345 - GFF2818 0.79 -0.4 245,345 - GFF2818 0.79 -2.0 245,345 - GFF2818 0.79 -0.1 245,811 + -0.4 245,812 - -0.8 245,812 - +1.5 245,812 - -0.2 245,812 - -0.7 245,812 - +0.5 245,984 + +0.1 245,985 - -1.3 245,985 - +0.3 245,985 - +0.2 245,985 - -0.3 245,985 - -0.0 246,329 - GFF2819 0.37 -0.8 246,393 + GFF2819 0.45 -1.7 246,393 + GFF2819 0.45 -1.4 246,393 + GFF2819 0.45 -0.5 246,393 + GFF2819 0.45 +0.2 246,393 + GFF2819 0.45 -0.5 246,394 - GFF2819 0.45 -1.5 246,522 + GFF2819 0.60 -0.5 246,522 + GFF2819 0.60 -2.1 246,523 - GFF2819 0.60 -2.0 246,558 + GFF2819 0.64 -2.0 246,846 + -0.1 246,846 + +0.4 246,847 - +0.8 246,914 + -0.5 246,914 + +1.5 246,915 - -1.1 246,915 - -0.8 246,915 - -0.2 246,915 - +0.9 246,915 - -0.3 246,915 - +0.5 247,045 + GFF2820 0.18 -0.4 247,045 + GFF2820 0.18 -0.1 247,045 + GFF2820 0.18 +1.5 247,045 + GFF2820 0.18 -1.1 247,046 - GFF2820 0.18 -0.6 247,046 - GFF2820 0.18 -0.3 247,046 - GFF2820 0.18 -0.3 247,046 - GFF2820 0.18 +0.1 247,046 - GFF2820 0.18 +0.1 247,046 - GFF2820 0.18 +0.6 247,046 - GFF2820 0.18 -0.2 247,059 - GFF2820 0.20 +0.2 247,248 + GFF2820 0.43 -0.7 247,249 - GFF2820 0.43 +0.1 247,249 - GFF2820 0.43 -0.7 247,379 + GFF2820 0.59 -0.6 247,380 - GFF2820 0.59 -1.0 247,644 + -0.4 247,644 + -0.5 247,707 + +0.2 247,707 + -1.4 247,707 + -2.0 247,707 + -0.0 247,707 + -1.3 247,707 + +0.5 247,708 - -0.4 247,708 - +1.0 247,708 - +0.2 247,708 - +1.0 247,708 - -2.1 247,708 - -0.7 247,708 - +1.5 247,708 - -0.3 247,708 - +0.8 247,708 - -0.7 247,708 - -0.4 247,708 - -1.8 247,708 - -0.6 247,708 - -0.4 247,817 + GFF2821 0.11 +1.1
Or see this region's nucleotide sequence