Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2652 and GFF2653 overlap by 4 nucleotides GFF2653 and GFF2654 are separated by 47 nucleotides
GFF2652 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 70,965 to 72,119
GFF2652
GFF2653 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 72,116 to 73,729
GFF2653
GFF2654 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydC subunit, at 73,777 to 75,492
GFF2654
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 71.329 kb on + strand, within GFF2652 at 71.329 kb on + strand, within GFF2652 at 71.461 kb on + strand, within GFF2652 at 71.461 kb on + strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.636 kb on - strand, within GFF2652 at 71.845 kb on + strand, within GFF2652 at 71.846 kb on - strand, within GFF2652 at 71.869 kb on + strand, within GFF2652 at 71.870 kb on - strand, within GFF2652 at 72.094 kb on + strand at 72.094 kb on + strand at 72.095 kb on - strand at 72.270 kb on + strand at 72.270 kb on + strand at 72.270 kb on + strand at 72.271 kb on - strand at 72.636 kb on + strand, within GFF2653 at 72.636 kb on + strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.754 kb on - strand, within GFF2653 at 72.754 kb on - strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 73.111 kb on - strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.457 kb on - strand, within GFF2653 at 73.477 kb on - strand, within GFF2653 at 73.479 kb on + strand, within GFF2653 at 73.480 kb on - strand, within GFF2653 at 73.608 kb on + strand at 73.609 kb on - strand at 73.674 kb on + strand at 73.675 kb on - strand at 73.675 kb on - strand at 73.745 kb on + strand at 73.745 kb on + strand at 73.746 kb on - strand at 73.746 kb on - strand at 73.746 kb on - strand at 74.583 kb on + strand, within GFF2654
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 71,329 + GFF2652 0.32 +0.3 71,329 + GFF2652 0.32 -0.4 71,461 + GFF2652 0.43 -1.3 71,461 + GFF2652 0.43 +0.2 71,462 - GFF2652 0.43 +0.4 71,462 - GFF2652 0.43 +0.2 71,462 - GFF2652 0.43 -2.0 71,462 - GFF2652 0.43 +0.2 71,462 - GFF2652 0.43 -0.3 71,497 + GFF2652 0.46 -0.4 71,497 + GFF2652 0.46 +0.0 71,497 + GFF2652 0.46 +1.8 71,636 - GFF2652 0.58 -0.1 71,845 + GFF2652 0.76 +1.0 71,846 - GFF2652 0.76 +1.0 71,869 + GFF2652 0.78 +1.3 71,870 - GFF2652 0.78 +2.8 72,094 + +0.5 72,094 + -0.6 72,095 - +1.5 72,270 + +0.6 72,270 + -0.8 72,270 + -1.3 72,271 - +0.4 72,636 + GFF2653 0.32 +0.5 72,636 + GFF2653 0.32 -0.6 72,637 - GFF2653 0.32 -0.6 72,637 - GFF2653 0.32 -0.8 72,637 - GFF2653 0.32 +0.2 72,754 - GFF2653 0.40 -1.3 72,754 - GFF2653 0.40 -1.3 72,876 + GFF2653 0.47 -0.4 72,876 + GFF2653 0.47 -1.0 72,876 + GFF2653 0.47 -1.1 73,111 - GFF2653 0.62 -0.1 73,152 + GFF2653 0.64 -0.6 73,152 + GFF2653 0.64 -1.1 73,152 + GFF2653 0.64 -0.5 73,153 - GFF2653 0.64 -2.1 73,153 - GFF2653 0.64 +0.0 73,457 - GFF2653 0.83 -0.3 73,477 - GFF2653 0.84 -0.4 73,479 + GFF2653 0.84 +0.2 73,480 - GFF2653 0.85 +0.4 73,608 + -0.3 73,609 - -1.5 73,674 + -0.3 73,675 - -1.3 73,675 - -1.2 73,745 + -0.8 73,745 + +0.7 73,746 - +0.1 73,746 - -0.3 73,746 - +0.7 74,583 + GFF2654 0.47 -0.8
Or see this region's nucleotide sequence