Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2573 and GFF2574 are separated by 26 nucleotides GFF2574 and GFF2575 are separated by 11 nucleotides GFF2575 and GFF2576 are separated by 117 nucleotides GFF2576 and GFF2577 are separated by 40 nucleotides
GFF2573 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 630,447 to 631,481
GFF2573
GFF2574 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 631,508 to 632,386
GFF2574
GFF2575 - Quinolinate synthetase (EC 2.5.1.72), at 632,398 to 633,534
GFF2575
GFF2576 - 6-carboxy-5,6,7,8-tetrahydropterin synthase (EC 4.1.2.50), at 633,652 to 634,014
GFF2576
GFF2577 - 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3), at 634,055 to 634,693
GFF2577
Position (kb) 632
633
634 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 631.880 kb on + strand, within GFF2574 at 631.880 kb on + strand, within GFF2574 at 631.881 kb on - strand, within GFF2574 at 631.961 kb on + strand, within GFF2574 at 631.961 kb on + strand, within GFF2574 at 631.962 kb on - strand, within GFF2574 at 631.962 kb on - strand, within GFF2574 at 631.962 kb on - strand, within GFF2574 at 632.006 kb on + strand, within GFF2574 at 632.006 kb on + strand, within GFF2574 at 632.006 kb on + strand, within GFF2574 at 632.007 kb on - strand, within GFF2574 at 632.007 kb on - strand, within GFF2574 at 632.007 kb on - strand, within GFF2574 at 632.317 kb on + strand at 632.317 kb on + strand at 632.318 kb on - strand at 632.318 kb on - strand at 632.318 kb on - strand at 632.318 kb on - strand at 632.360 kb on + strand at 632.360 kb on + strand at 632.361 kb on - strand at 632.361 kb on - strand at 632.428 kb on + strand at 632.429 kb on - strand at 632.429 kb on - strand at 632.429 kb on - strand at 632.429 kb on - strand at 632.528 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.599 kb on + strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.600 kb on - strand, within GFF2575 at 632.869 kb on + strand, within GFF2575 at 632.869 kb on + strand, within GFF2575 at 632.869 kb on + strand, within GFF2575 at 632.870 kb on - strand, within GFF2575 at 632.870 kb on - strand, within GFF2575 at 632.870 kb on - strand, within GFF2575 at 632.870 kb on - strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.004 kb on + strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.005 kb on - strand, within GFF2575 at 633.267 kb on + strand, within GFF2575 at 633.267 kb on + strand, within GFF2575 at 633.267 kb on + strand, within GFF2575 at 633.267 kb on + strand, within GFF2575 at 633.268 kb on - strand, within GFF2575 at 633.268 kb on - strand, within GFF2575 at 633.303 kb on + strand, within GFF2575 at 633.304 kb on - strand, within GFF2575 at 633.304 kb on - strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.357 kb on + strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.358 kb on - strand, within GFF2575 at 633.653 kb on + strand at 633.653 kb on + strand at 633.654 kb on - strand at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.914 kb on + strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.915 kb on - strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.930 kb on + strand, within GFF2576 at 633.931 kb on - strand, within GFF2576
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 631,880 + GFF2574 0.42 -0.8 631,880 + GFF2574 0.42 -1.5 631,881 - GFF2574 0.42 -1.7 631,961 + GFF2574 0.52 -0.4 631,961 + GFF2574 0.52 -0.5 631,962 - GFF2574 0.52 -2.3 631,962 - GFF2574 0.52 -2.5 631,962 - GFF2574 0.52 +0.6 632,006 + GFF2574 0.57 -0.7 632,006 + GFF2574 0.57 -1.6 632,006 + GFF2574 0.57 -3.2 632,007 - GFF2574 0.57 +0.1 632,007 - GFF2574 0.57 -2.6 632,007 - GFF2574 0.57 -1.5 632,317 + -1.9 632,317 + -1.1 632,318 - -1.1 632,318 - -2.5 632,318 - -1.8 632,318 - -3.6 632,360 + -0.8 632,360 + -0.3 632,361 - -1.7 632,361 - -0.1 632,428 + -2.2 632,429 - -1.7 632,429 - -2.0 632,429 - +0.2 632,429 - -2.4 632,528 + GFF2575 0.11 -1.0 632,599 + GFF2575 0.18 -2.9 632,599 + GFF2575 0.18 -0.4 632,599 + GFF2575 0.18 -2.4 632,599 + GFF2575 0.18 -1.5 632,599 + GFF2575 0.18 -1.5 632,599 + GFF2575 0.18 -3.0 632,599 + GFF2575 0.18 -2.3 632,599 + GFF2575 0.18 -1.9 632,599 + GFF2575 0.18 -1.1 632,600 - GFF2575 0.18 -2.5 632,600 - GFF2575 0.18 -1.3 632,600 - GFF2575 0.18 -0.7 632,600 - GFF2575 0.18 -1.8 632,600 - GFF2575 0.18 -0.3 632,600 - GFF2575 0.18 -0.3 632,600 - GFF2575 0.18 -0.2 632,600 - GFF2575 0.18 -0.6 632,600 - GFF2575 0.18 -0.9 632,600 - GFF2575 0.18 -2.0 632,600 - GFF2575 0.18 -2.9 632,600 - GFF2575 0.18 -2.4 632,600 - GFF2575 0.18 -2.1 632,600 - GFF2575 0.18 -1.2 632,600 - GFF2575 0.18 -2.4 632,600 - GFF2575 0.18 -2.6 632,869 + GFF2575 0.41 -0.8 632,869 + GFF2575 0.41 -1.7 632,869 + GFF2575 0.41 -1.8 632,870 - GFF2575 0.42 -3.1 632,870 - GFF2575 0.42 -1.6 632,870 - GFF2575 0.42 -4.0 632,870 - GFF2575 0.42 -0.9 633,004 + GFF2575 0.53 -1.1 633,004 + GFF2575 0.53 -1.2 633,004 + GFF2575 0.53 -1.6 633,004 + GFF2575 0.53 -1.9 633,004 + GFF2575 0.53 -2.2 633,004 + GFF2575 0.53 -1.8 633,004 + GFF2575 0.53 +1.4 633,005 - GFF2575 0.53 -1.5 633,005 - GFF2575 0.53 -2.0 633,005 - GFF2575 0.53 -1.2 633,005 - GFF2575 0.53 -1.8 633,005 - GFF2575 0.53 -0.7 633,005 - GFF2575 0.53 -2.2 633,005 - GFF2575 0.53 -1.1 633,005 - GFF2575 0.53 -1.9 633,005 - GFF2575 0.53 -1.8 633,005 - GFF2575 0.53 -1.8 633,005 - GFF2575 0.53 -1.7 633,005 - GFF2575 0.53 -0.7 633,005 - GFF2575 0.53 -0.7 633,005 - GFF2575 0.53 -1.5 633,267 + GFF2575 0.76 -2.2 633,267 + GFF2575 0.76 -2.7 633,267 + GFF2575 0.76 -2.9 633,267 + GFF2575 0.76 -2.4 633,268 - GFF2575 0.77 -2.0 633,268 - GFF2575 0.77 -2.9 633,303 + GFF2575 0.80 -1.9 633,304 - GFF2575 0.80 -1.1 633,304 - GFF2575 0.80 -1.8 633,357 + GFF2575 0.84 +0.1 633,357 + GFF2575 0.84 -2.2 633,357 + GFF2575 0.84 -1.5 633,357 + GFF2575 0.84 -1.0 633,357 + GFF2575 0.84 -1.6 633,357 + GFF2575 0.84 -2.7 633,357 + GFF2575 0.84 -2.9 633,358 - GFF2575 0.84 -2.2 633,358 - GFF2575 0.84 -1.5 633,358 - GFF2575 0.84 -0.9 633,358 - GFF2575 0.84 -2.2 633,358 - GFF2575 0.84 -1.9 633,358 - GFF2575 0.84 -2.8 633,358 - GFF2575 0.84 -2.2 633,358 - GFF2575 0.84 +0.1 633,358 - GFF2575 0.84 -2.5 633,653 + -1.7 633,653 + +0.2 633,654 - +0.6 633,914 + GFF2576 0.72 -0.9 633,914 + GFF2576 0.72 +1.2 633,914 + GFF2576 0.72 +0.5 633,914 + GFF2576 0.72 +1.4 633,914 + GFF2576 0.72 +1.2 633,914 + GFF2576 0.72 +0.4 633,914 + GFF2576 0.72 +0.6 633,914 + GFF2576 0.72 +2.5 633,914 + GFF2576 0.72 +0.7 633,915 - GFF2576 0.72 +0.7 633,915 - GFF2576 0.72 +0.7 633,915 - GFF2576 0.72 -1.9 633,915 - GFF2576 0.72 +0.7 633,915 - GFF2576 0.72 -0.7 633,930 + GFF2576 0.77 -0.7 633,930 + GFF2576 0.77 +0.6 633,931 - GFF2576 0.77 +1.4
Or see this region's nucleotide sequence