Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2365 and GFF2366 are separated by 24 nucleotides GFF2366 and GFF2367 are separated by 130 nucleotides
GFF2365 - Altronate dehydratase (EC 4.2.1.7), at 418,721 to 420,244
GFF2365
GFF2366 - Tricarboxylate transport protein TctC, at 420,269 to 421,267
GFF2366
GFF2367 - Transcriptional regulator, LysR family, at 421,398 to 422,333
GFF2367
Position (kb)
420
421
422 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 419.682 kb on + strand, within GFF2365 at 419.682 kb on + strand, within GFF2365 at 419.683 kb on - strand, within GFF2365 at 419.943 kb on + strand, within GFF2365 at 419.943 kb on + strand, within GFF2365 at 419.944 kb on - strand, within GFF2365 at 419.986 kb on - strand, within GFF2365 at 419.986 kb on - strand, within GFF2365 at 420.021 kb on + strand, within GFF2365 at 420.021 kb on + strand, within GFF2365 at 420.021 kb on + strand, within GFF2365 at 420.070 kb on - strand, within GFF2365 at 420.070 kb on - strand, within GFF2365 at 420.330 kb on + strand at 420.355 kb on - strand at 420.372 kb on + strand, within GFF2366 at 420.372 kb on + strand, within GFF2366 at 420.372 kb on + strand, within GFF2366 at 420.373 kb on - strand, within GFF2366 at 420.373 kb on - strand, within GFF2366 at 420.542 kb on - strand, within GFF2366 at 420.697 kb on - strand, within GFF2366 at 420.697 kb on - strand, within GFF2366 at 420.784 kb on - strand, within GFF2366 at 420.784 kb on - strand, within GFF2366 at 420.897 kb on + strand, within GFF2366 at 420.965 kb on + strand, within GFF2366 at 420.965 kb on + strand, within GFF2366 at 420.966 kb on - strand, within GFF2366 at 420.966 kb on - strand, within GFF2366 at 420.981 kb on + strand, within GFF2366 at 420.981 kb on + strand, within GFF2366 at 421.164 kb on + strand, within GFF2366 at 421.362 kb on + strand at 421.608 kb on + strand, within GFF2367 at 422.206 kb on + strand, within GFF2367 at 422.206 kb on + strand, within GFF2367 at 422.207 kb on - strand, within GFF2367 at 422.207 kb on - strand, within GFF2367
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 419,682 + GFF2365 0.63 -0.1 419,682 + GFF2365 0.63 +0.8 419,683 - GFF2365 0.63 -1.0 419,943 + GFF2365 0.80 -1.2 419,943 + GFF2365 0.80 -0.1 419,944 - GFF2365 0.80 -0.1 419,986 - GFF2365 0.83 +0.9 419,986 - GFF2365 0.83 -0.7 420,021 + GFF2365 0.85 +0.5 420,021 + GFF2365 0.85 +0.2 420,021 + GFF2365 0.85 +0.5 420,070 - GFF2365 0.89 -0.6 420,070 - GFF2365 0.89 +0.2 420,330 + -0.8 420,355 - -0.3 420,372 + GFF2366 0.10 +2.7 420,372 + GFF2366 0.10 +0.1 420,372 + GFF2366 0.10 +0.0 420,373 - GFF2366 0.10 -0.6 420,373 - GFF2366 0.10 +0.4 420,542 - GFF2366 0.27 -0.5 420,697 - GFF2366 0.43 +0.7 420,697 - GFF2366 0.43 -1.7 420,784 - GFF2366 0.52 +0.1 420,784 - GFF2366 0.52 -1.0 420,897 + GFF2366 0.63 +0.1 420,965 + GFF2366 0.70 -0.5 420,965 + GFF2366 0.70 -0.1 420,966 - GFF2366 0.70 -0.8 420,966 - GFF2366 0.70 -0.1 420,981 + GFF2366 0.71 -1.8 420,981 + GFF2366 0.71 +0.7 421,164 + GFF2366 0.90 -0.1 421,362 + -0.7 421,608 + GFF2367 0.22 -0.2 422,206 + GFF2367 0.86 -0.9 422,206 + GFF2367 0.86 -0.9 422,207 - GFF2367 0.86 +0.4 422,207 - GFF2367 0.86 +0.3
Or see this region's nucleotide sequence