Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2304 and GFF2305 overlap by 14 nucleotides GFF2305 and GFF2306 are separated by 24 nucleotides GFF2306 and GFF2307 overlap by 4 nucleotides GFF2307 and GFF2308 are separated by 13 nucleotides
GFF2304 - Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion, at 355,687 to 356,541
GFF2304
GFF2305 - Hypothetical protein in cytochrome oxidase biogenesis cluster, at 356,528 to 357,208
GFF2305
GFF2306 - Heme A synthase, cytochrome oxidase biogenesis protein Cox15-CtaA, at 357,233 to 358,468
GFF2306
GFF2307 - Heme O synthase, protoheme IX farnesyltransferase, COX10-CtaB, at 358,465 to 359,370
GFF2307
GFF2308 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, thiol-disulfide reductase involved in Cu(I) insertion into CoxII Cu(A) center, at 359,384 to 360,001
GFF2308
Position (kb) 357
358
359 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 356.269 kb on + strand, within GFF2304 at 356.270 kb on - strand, within GFF2304 at 356.335 kb on - strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.422 kb on + strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.423 kb on - strand, within GFF2304 at 356.429 kb on - strand, within GFF2304 at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.473 kb on + strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.474 kb on - strand at 356.681 kb on + strand, within GFF2305 at 356.681 kb on + strand, within GFF2305 at 356.681 kb on + strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.682 kb on - strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.690 kb on + strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.691 kb on - strand, within GFF2305 at 356.692 kb on - strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.693 kb on + strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.694 kb on - strand, within GFF2305 at 356.724 kb on - strand, within GFF2305 at 356.876 kb on + strand, within GFF2305 at 356.876 kb on + strand, within GFF2305 at 356.876 kb on + strand, within GFF2305 at 356.876 kb on + strand, within GFF2305 at 356.877 kb on - strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.074 kb on + strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.075 kb on - strand, within GFF2305 at 357.255 kb on - strand at 357.255 kb on - strand at 357.473 kb on + strand, within GFF2306 at 357.584 kb on + strand, within GFF2306 at 357.584 kb on + strand, within GFF2306 at 357.584 kb on + strand, within GFF2306 at 357.869 kb on + strand, within GFF2306 at 357.906 kb on + strand, within GFF2306 at 357.906 kb on + strand, within GFF2306 at 357.906 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.992 kb on + strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 357.993 kb on - strand, within GFF2306 at 358.142 kb on + strand, within GFF2306 at 358.142 kb on + strand, within GFF2306 at 358.142 kb on + strand, within GFF2306 at 358.142 kb on + strand, within GFF2306 at 358.143 kb on - strand, within GFF2306 at 358.143 kb on - strand, within GFF2306 at 358.143 kb on - strand, within GFF2306 at 358.143 kb on - strand, within GFF2306 at 358.143 kb on - strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.163 kb on + strand, within GFF2306 at 358.164 kb on - strand, within GFF2306 at 358.164 kb on - strand, within GFF2306 at 358.470 kb on + strand at 358.470 kb on + strand at 358.470 kb on + strand at 358.470 kb on + strand at 358.471 kb on - strand at 358.522 kb on + strand at 358.522 kb on + strand at 358.522 kb on + strand at 358.523 kb on - strand at 358.882 kb on + strand, within GFF2307 at 358.882 kb on + strand, within GFF2307 at 358.882 kb on + strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 358.883 kb on - strand, within GFF2307 at 359.060 kb on - strand, within GFF2307 at 359.060 kb on - strand, within GFF2307 at 359.074 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.146 kb on + strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.147 kb on - strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.188 kb on + strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.189 kb on - strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.209 kb on + strand, within GFF2307 at 359.210 kb on - strand, within GFF2307 at 359.210 kb on - strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.275 kb on + strand, within GFF2307 at 359.276 kb on - strand, within GFF2307 at 359.276 kb on - strand, within GFF2307 at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.359 kb on + strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.360 kb on - strand at 359.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 356,269 + GFF2304 0.68 +1.1 356,270 - GFF2304 0.68 -0.3 356,335 - GFF2304 0.76 -0.5 356,422 + GFF2304 0.86 -0.1 356,422 + GFF2304 0.86 -0.5 356,422 + GFF2304 0.86 -0.7 356,422 + GFF2304 0.86 -2.7 356,422 + GFF2304 0.86 -1.6 356,422 + GFF2304 0.86 -1.1 356,422 + GFF2304 0.86 +0.5 356,422 + GFF2304 0.86 -1.3 356,422 + GFF2304 0.86 -0.9 356,423 - GFF2304 0.86 -1.2 356,423 - GFF2304 0.86 -0.1 356,423 - GFF2304 0.86 -2.6 356,423 - GFF2304 0.86 -1.4 356,429 - GFF2304 0.87 -1.9 356,473 + -0.5 356,473 + -1.6 356,473 + -0.3 356,473 + -0.8 356,473 + -1.1 356,473 + -0.6 356,473 + -1.4 356,473 + -2.3 356,474 - -1.1 356,474 - -1.1 356,474 - +0.9 356,474 - +0.1 356,474 - -0.7 356,474 - -1.0 356,474 - -0.9 356,474 - -2.4 356,474 - -0.5 356,474 - -1.8 356,474 - -0.4 356,474 - -0.7 356,474 - -1.1 356,474 - -0.9 356,681 + GFF2305 0.22 -0.3 356,681 + GFF2305 0.22 -0.1 356,681 + GFF2305 0.22 +0.3 356,682 - GFF2305 0.23 +0.1 356,682 - GFF2305 0.23 -0.4 356,682 - GFF2305 0.23 -0.6 356,682 - GFF2305 0.23 -1.2 356,682 - GFF2305 0.23 -0.1 356,682 - GFF2305 0.23 -0.1 356,682 - GFF2305 0.23 +0.8 356,690 + GFF2305 0.24 -0.7 356,690 + GFF2305 0.24 -0.6 356,690 + GFF2305 0.24 -0.3 356,690 + GFF2305 0.24 +0.2 356,690 + GFF2305 0.24 -0.3 356,690 + GFF2305 0.24 +0.1 356,690 + GFF2305 0.24 +0.9 356,691 - GFF2305 0.24 +0.1 356,691 - GFF2305 0.24 +0.5 356,691 - GFF2305 0.24 -0.2 356,691 - GFF2305 0.24 +0.1 356,691 - GFF2305 0.24 -0.1 356,691 - GFF2305 0.24 -0.8 356,691 - GFF2305 0.24 -0.3 356,691 - GFF2305 0.24 -0.2 356,691 - GFF2305 0.24 +0.6 356,692 - GFF2305 0.24 -0.1 356,693 + GFF2305 0.24 -0.9 356,693 + GFF2305 0.24 -0.7 356,693 + GFF2305 0.24 -0.2 356,693 + GFF2305 0.24 -0.8 356,693 + GFF2305 0.24 -1.1 356,693 + GFF2305 0.24 -0.2 356,693 + GFF2305 0.24 -0.6 356,693 + GFF2305 0.24 -1.0 356,693 + GFF2305 0.24 -0.1 356,693 + GFF2305 0.24 -0.6 356,693 + GFF2305 0.24 +0.2 356,693 + GFF2305 0.24 +0.7 356,693 + GFF2305 0.24 -0.4 356,693 + GFF2305 0.24 +1.3 356,694 - GFF2305 0.24 +1.7 356,694 - GFF2305 0.24 -0.6 356,694 - GFF2305 0.24 -0.2 356,694 - GFF2305 0.24 +0.7 356,694 - GFF2305 0.24 +0.0 356,694 - GFF2305 0.24 +0.2 356,694 - GFF2305 0.24 -2.3 356,694 - GFF2305 0.24 +1.3 356,694 - GFF2305 0.24 -0.8 356,694 - GFF2305 0.24 -0.3 356,694 - GFF2305 0.24 -1.5 356,694 - GFF2305 0.24 -1.4 356,694 - GFF2305 0.24 -0.4 356,724 - GFF2305 0.29 -0.4 356,876 + GFF2305 0.51 +0.2 356,876 + GFF2305 0.51 -0.3 356,876 + GFF2305 0.51 +0.1 356,876 + GFF2305 0.51 -0.1 356,877 - GFF2305 0.51 -0.5 357,074 + GFF2305 0.80 -0.4 357,074 + GFF2305 0.80 +0.2 357,074 + GFF2305 0.80 -1.0 357,074 + GFF2305 0.80 +0.1 357,074 + GFF2305 0.80 -1.2 357,074 + GFF2305 0.80 +0.0 357,074 + GFF2305 0.80 -0.9 357,074 + GFF2305 0.80 -0.4 357,074 + GFF2305 0.80 -0.5 357,074 + GFF2305 0.80 +0.1 357,074 + GFF2305 0.80 +0.0 357,074 + GFF2305 0.80 +0.7 357,074 + GFF2305 0.80 +0.7 357,074 + GFF2305 0.80 -0.3 357,074 + GFF2305 0.80 -1.5 357,074 + GFF2305 0.80 +0.9 357,074 + GFF2305 0.80 +0.5 357,075 - GFF2305 0.80 -0.1 357,075 - GFF2305 0.80 -0.6 357,075 - GFF2305 0.80 +1.1 357,075 - GFF2305 0.80 +0.3 357,075 - GFF2305 0.80 +1.0 357,075 - GFF2305 0.80 -0.3 357,075 - GFF2305 0.80 -1.2 357,075 - GFF2305 0.80 +0.4 357,255 - -0.3 357,255 - -0.1 357,473 + GFF2306 0.19 -0.6 357,584 + GFF2306 0.28 -1.3 357,584 + GFF2306 0.28 -0.8 357,584 + GFF2306 0.28 -1.5 357,869 + GFF2306 0.51 -0.2 357,906 + GFF2306 0.54 -0.8 357,906 + GFF2306 0.54 -1.1 357,906 + GFF2306 0.54 -1.0 357,992 + GFF2306 0.61 -0.5 357,992 + GFF2306 0.61 -0.9 357,992 + GFF2306 0.61 +0.7 357,992 + GFF2306 0.61 -2.1 357,992 + GFF2306 0.61 -1.4 357,992 + GFF2306 0.61 -1.7 357,992 + GFF2306 0.61 -1.6 357,993 - GFF2306 0.61 -0.4 357,993 - GFF2306 0.61 +0.5 357,993 - GFF2306 0.61 -1.3 357,993 - GFF2306 0.61 -0.0 357,993 - GFF2306 0.61 -0.8 357,993 - GFF2306 0.61 -1.2 357,993 - GFF2306 0.61 -0.5 357,993 - GFF2306 0.61 -1.3 357,993 - GFF2306 0.61 -1.1 357,993 - GFF2306 0.61 -0.6 357,993 - GFF2306 0.61 +0.4 358,142 + GFF2306 0.74 -2.1 358,142 + GFF2306 0.74 -2.0 358,142 + GFF2306 0.74 -2.2 358,142 + GFF2306 0.74 -1.6 358,143 - GFF2306 0.74 -0.5 358,143 - GFF2306 0.74 -1.5 358,143 - GFF2306 0.74 -1.4 358,143 - GFF2306 0.74 -0.0 358,143 - GFF2306 0.74 +0.1 358,163 + GFF2306 0.75 -1.6 358,163 + GFF2306 0.75 -1.1 358,163 + GFF2306 0.75 -0.3 358,163 + GFF2306 0.75 -1.7 358,163 + GFF2306 0.75 -1.1 358,163 + GFF2306 0.75 -1.8 358,164 - GFF2306 0.75 -1.7 358,164 - GFF2306 0.75 -0.8 358,470 + +0.1 358,470 + -1.2 358,470 + -1.0 358,470 + -0.2 358,471 - -1.2 358,522 + +0.5 358,522 + +0.4 358,522 + -0.9 358,523 - +0.0 358,882 + GFF2307 0.46 -0.6 358,882 + GFF2307 0.46 -1.1 358,882 + GFF2307 0.46 -1.5 358,883 - GFF2307 0.46 -1.1 358,883 - GFF2307 0.46 -0.6 358,883 - GFF2307 0.46 -0.8 358,883 - GFF2307 0.46 -2.5 358,883 - GFF2307 0.46 -1.2 358,883 - GFF2307 0.46 -1.6 358,883 - GFF2307 0.46 +0.6 359,060 - GFF2307 0.66 +0.1 359,060 - GFF2307 0.66 -3.6 359,074 + GFF2307 0.67 -2.1 359,146 + GFF2307 0.75 -0.6 359,146 + GFF2307 0.75 -0.3 359,146 + GFF2307 0.75 -1.6 359,146 + GFF2307 0.75 -1.5 359,146 + GFF2307 0.75 +1.2 359,147 - GFF2307 0.75 -0.5 359,147 - GFF2307 0.75 -0.3 359,147 - GFF2307 0.75 -2.6 359,147 - GFF2307 0.75 +1.1 359,147 - GFF2307 0.75 +0.2 359,188 + GFF2307 0.80 -0.3 359,188 + GFF2307 0.80 -0.7 359,188 + GFF2307 0.80 -1.5 359,189 - GFF2307 0.80 -0.3 359,189 - GFF2307 0.80 +0.1 359,189 - GFF2307 0.80 +0.3 359,189 - GFF2307 0.80 -0.4 359,189 - GFF2307 0.80 -1.5 359,209 + GFF2307 0.82 -1.0 359,209 + GFF2307 0.82 -1.0 359,209 + GFF2307 0.82 -0.2 359,209 + GFF2307 0.82 +1.3 359,209 + GFF2307 0.82 +0.7 359,210 - GFF2307 0.82 -3.4 359,210 - GFF2307 0.82 -1.5 359,275 + GFF2307 0.89 -1.1 359,275 + GFF2307 0.89 -0.2 359,275 + GFF2307 0.89 -1.0 359,276 - GFF2307 0.90 +0.8 359,276 - GFF2307 0.90 +1.0 359,359 + -0.2 359,359 + -1.4 359,359 + +0.9 359,359 + -1.3 359,359 + -0.7 359,359 + -0.6 359,359 + -0.6 359,359 + +0.9 359,359 + +0.5 359,359 + -1.3 359,359 + +0.2 359,359 + -0.3 359,359 + +0.8 359,359 + -0.2 359,359 + -0.9 359,360 - +0.9 359,360 - -0.7 359,360 - -0.3 359,360 - -0.3 359,360 - -0.3 359,360 - +0.5 359,360 - +0.5 359,360 - +1.4 359,360 - +0.1 359,360 - -1.3 359,360 - +1.1 359,360 - -0.4 359,360 - +0.7 359,360 - -0.5 359,360 - -0.6 359,364 - -0.8
Or see this region's nucleotide sequence