Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2276 and GFF2277 are separated by 50 nucleotides GFF2277 and GFF2278 are separated by 1 nucleotides GFF2278 and GFF2279 are separated by 1 nucleotides
GFF2276 - Chlorogenate esterase, at 329,371 to 331,110
GFF2276
GFF2277 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 331,161 to 331,895
GFF2277
GFF2278 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 331,897 to 333,711
GFF2278
GFF2279 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 333,713 to 334,753
GFF2279
Position (kb) 331
332
333
334 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 330.924 kb on - strand, within GFF2276 at 330.924 kb on - strand, within GFF2276 at 331.189 kb on + strand at 331.189 kb on + strand at 331.189 kb on + strand at 331.189 kb on + strand at 331.190 kb on - strand at 331.190 kb on - strand at 331.190 kb on - strand at 331.190 kb on - strand at 331.190 kb on - strand at 331.190 kb on - strand at 331.267 kb on + strand, within GFF2277 at 331.267 kb on + strand, within GFF2277 at 331.267 kb on + strand, within GFF2277 at 331.268 kb on - strand, within GFF2277 at 331.268 kb on - strand, within GFF2277 at 331.528 kb on + strand, within GFF2277 at 331.528 kb on + strand, within GFF2277 at 331.528 kb on + strand, within GFF2277 at 331.528 kb on + strand, within GFF2277 at 331.529 kb on - strand, within GFF2277 at 331.529 kb on - strand, within GFF2277 at 332.765 kb on + strand, within GFF2278 at 332.766 kb on - strand, within GFF2278 at 332.766 kb on - strand, within GFF2278 at 332.810 kb on + strand, within GFF2278 at 332.811 kb on - strand, within GFF2278 at 332.811 kb on - strand, within GFF2278 at 333.195 kb on - strand, within GFF2278 at 333.195 kb on - strand, within GFF2278 at 333.263 kb on + strand, within GFF2278 at 333.263 kb on + strand, within GFF2278 at 333.264 kb on - strand, within GFF2278 at 333.296 kb on + strand, within GFF2278 at 333.296 kb on + strand, within GFF2278 at 333.297 kb on - strand, within GFF2278 at 333.297 kb on - strand, within GFF2278 at 333.297 kb on - strand, within GFF2278 at 333.299 kb on + strand, within GFF2278 at 333.299 kb on + strand, within GFF2278 at 333.338 kb on + strand, within GFF2278 at 333.338 kb on + strand, within GFF2278 at 333.339 kb on - strand, within GFF2278 at 333.452 kb on + strand, within GFF2278 at 333.464 kb on + strand, within GFF2278 at 333.465 kb on - strand, within GFF2278 at 333.465 kb on - strand, within GFF2278 at 333.465 kb on - strand, within GFF2278 at 333.554 kb on + strand at 333.555 kb on - strand at 333.555 kb on - strand at 333.555 kb on - strand at 333.566 kb on + strand at 333.566 kb on + strand at 333.567 kb on - strand at 333.567 kb on - strand at 333.567 kb on - strand at 333.870 kb on + strand, within GFF2279 at 333.937 kb on - strand, within GFF2279 at 333.937 kb on - strand, within GFF2279 at 333.937 kb on - strand, within GFF2279 at 334.090 kb on - strand, within GFF2279 at 334.326 kb on + strand, within GFF2279 at 334.327 kb on - strand, within GFF2279 at 334.327 kb on - strand, within GFF2279 at 334.327 kb on - strand, within GFF2279 at 334.614 kb on + strand, within GFF2279 at 334.695 kb on + strand at 334.695 kb on + strand at 334.695 kb on + strand at 334.695 kb on + strand at 334.695 kb on + strand at 334.696 kb on - strand at 334.696 kb on - strand at 334.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 330,924 - GFF2276 0.89 -0.8 330,924 - GFF2276 0.89 +1.0 331,189 + -0.9 331,189 + +0.5 331,189 + -0.2 331,189 + +1.3 331,190 - +0.6 331,190 - +0.9 331,190 - +0.0 331,190 - +0.7 331,190 - +0.1 331,190 - -0.4 331,267 + GFF2277 0.14 -0.5 331,267 + GFF2277 0.14 -0.2 331,267 + GFF2277 0.14 -0.7 331,268 - GFF2277 0.15 -0.7 331,268 - GFF2277 0.15 +0.1 331,528 + GFF2277 0.50 -0.5 331,528 + GFF2277 0.50 +0.4 331,528 + GFF2277 0.50 -0.1 331,528 + GFF2277 0.50 -0.2 331,529 - GFF2277 0.50 -0.3 331,529 - GFF2277 0.50 -0.3 332,765 + GFF2278 0.48 -0.8 332,766 - GFF2278 0.48 -0.2 332,766 - GFF2278 0.48 +0.5 332,810 + GFF2278 0.50 -0.3 332,811 - GFF2278 0.50 -0.2 332,811 - GFF2278 0.50 -0.4 333,195 - GFF2278 0.72 +0.9 333,195 - GFF2278 0.72 -0.1 333,263 + GFF2278 0.75 +0.6 333,263 + GFF2278 0.75 +0.5 333,264 - GFF2278 0.75 -0.1 333,296 + GFF2278 0.77 +0.1 333,296 + GFF2278 0.77 -1.1 333,297 - GFF2278 0.77 -0.1 333,297 - GFF2278 0.77 +0.3 333,297 - GFF2278 0.77 -0.2 333,299 + GFF2278 0.77 -0.3 333,299 + GFF2278 0.77 +0.4 333,338 + GFF2278 0.79 -0.2 333,338 + GFF2278 0.79 +1.1 333,339 - GFF2278 0.79 -0.3 333,452 + GFF2278 0.86 -0.9 333,464 + GFF2278 0.86 +0.4 333,465 - GFF2278 0.86 -0.1 333,465 - GFF2278 0.86 -0.4 333,465 - GFF2278 0.86 +0.1 333,554 + +1.0 333,555 - +1.3 333,555 - -0.2 333,555 - +2.4 333,566 + +0.3 333,566 + -0.6 333,567 - -0.4 333,567 - +2.5 333,567 - +2.7 333,870 + GFF2279 0.15 +2.0 333,937 - GFF2279 0.22 +0.3 333,937 - GFF2279 0.22 +0.7 333,937 - GFF2279 0.22 -0.1 334,090 - GFF2279 0.36 -0.1 334,326 + GFF2279 0.59 +0.5 334,327 - GFF2279 0.59 +0.2 334,327 - GFF2279 0.59 +3.1 334,327 - GFF2279 0.59 +0.2 334,614 + GFF2279 0.87 -0.8 334,695 + -1.9 334,695 + -1.1 334,695 + -0.4 334,695 + +0.4 334,695 + +0.7 334,696 - -0.2 334,696 - -0.1 334,696 - -0.4
Or see this region's nucleotide sequence