Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2228 and GFF2229 are separated by 79 nucleotides GFF2229 and GFF2230 are separated by 136 nucleotides GFF2230 and GFF2231 overlap by 4 nucleotides
GFF2228 - glycerophosphoryl diester phosphodiesterase, at 289,607 to 291,082
GFF2228
GFF2229 - Pca regulon regulatory protein PcaR, at 291,162 to 291,947
GFF2229
GFF2230 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6), at 292,084 to 292,785
GFF2230
GFF2231 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6), at 292,782 to 293,429
GFF2231
Position (kb)
291
292 Strain fitness (log2 ratio)
-1
0
1
2 at 290.334 kb on + strand, within GFF2228 at 290.334 kb on + strand, within GFF2228 at 290.334 kb on + strand, within GFF2228 at 290.335 kb on - strand, within GFF2228 at 290.335 kb on - strand, within GFF2228 at 290.490 kb on + strand, within GFF2228 at 290.491 kb on - strand, within GFF2228 at 290.500 kb on - strand, within GFF2228 at 290.643 kb on + strand, within GFF2228 at 290.644 kb on - strand, within GFF2228 at 290.685 kb on + strand, within GFF2228 at 291.508 kb on + strand, within GFF2229 at 291.577 kb on + strand, within GFF2229 at 291.578 kb on - strand, within GFF2229 at 291.688 kb on + strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 292.460 kb on - strand, within GFF2230 at 292.592 kb on - strand, within GFF2230 at 292.653 kb on + strand, within GFF2230 at 292.794 kb on + strand at 292.794 kb on + strand at 292.794 kb on + strand at 292.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 290,334 + GFF2228 0.49 +2.4 290,334 + GFF2228 0.49 +0.5 290,334 + GFF2228 0.49 -0.4 290,335 - GFF2228 0.49 +0.3 290,335 - GFF2228 0.49 -0.9 290,490 + GFF2228 0.60 -0.3 290,491 - GFF2228 0.60 +0.3 290,500 - GFF2228 0.61 +1.5 290,643 + GFF2228 0.70 -0.2 290,644 - GFF2228 0.70 +0.1 290,685 + GFF2228 0.73 -0.1 291,508 + GFF2229 0.44 -0.2 291,577 + GFF2229 0.53 -1.1 291,578 - GFF2229 0.53 -0.8 291,688 + GFF2229 0.67 -0.5 291,689 - GFF2229 0.67 -1.2 291,689 - GFF2229 0.67 +0.5 291,689 - GFF2229 0.67 -0.4 291,898 + +1.7 291,898 + +0.1 291,898 + -0.6 291,898 + +0.1 291,898 + -1.6 291,898 + -0.1 291,898 + +0.4 291,898 + -0.4 291,898 + -0.2 291,898 + -1.5 291,898 + -1.4 291,899 - +1.9 291,899 - -0.6 291,899 - -0.5 291,899 - +0.1 291,899 - +0.1 291,899 - -0.2 291,899 - +2.3 292,460 - GFF2230 0.54 +0.1 292,592 - GFF2230 0.72 +0.5 292,653 + GFF2230 0.81 -0.3 292,794 + +0.7 292,794 + +1.7 292,794 + +0.7 292,795 - -0.3
Or see this region's nucleotide sequence