Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2118 and GFF2119 overlap by 4 nucleotides GFF2119 and GFF2120 overlap by 4 nucleotides GFF2120 and GFF2121 are separated by 25 nucleotides
GFF2118 - hypothetical protein, at 167,903 to 168,460
GFF2118
GFF2119 - Type IV fimbrial biogenesis protein PilW, at 168,457 to 169,221
GFF2119
GFF2120 - Type IV fimbrial biogenesis protein PilX, at 169,218 to 169,799
GFF2120
GFF2121 - Type IV fimbrial biogenesis protein PilY1, at 169,825 to 173,256
GFF2121
Position (kb) 169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 168.245 kb on + strand, within GFF2118 at 168.290 kb on - strand, within GFF2118 at 168.293 kb on + strand, within GFF2118 at 168.371 kb on + strand, within GFF2118 at 168.372 kb on - strand, within GFF2118 at 168.447 kb on - strand at 168.829 kb on + strand, within GFF2119 at 168.829 kb on + strand, within GFF2119 at 168.915 kb on + strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 168.916 kb on - strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.027 kb on + strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.028 kb on - strand, within GFF2119 at 169.235 kb on + strand at 169.338 kb on + strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.339 kb on - strand, within GFF2120 at 169.507 kb on + strand, within GFF2120 at 169.563 kb on + strand, within GFF2120 at 169.629 kb on + strand, within GFF2120 at 169.630 kb on - strand, within GFF2120 at 169.633 kb on - strand, within GFF2120 at 169.660 kb on - strand, within GFF2120 at 169.758 kb on + strand at 169.759 kb on - strand at 169.942 kb on + strand at 169.942 kb on + strand at 169.943 kb on - strand at 170.029 kb on + strand at 170.029 kb on + strand at 170.029 kb on + strand at 170.030 kb on - strand at 170.033 kb on - strand at 170.033 kb on - strand at 170.033 kb on - strand at 170.126 kb on + strand at 170.198 kb on + strand, within GFF2121 at 170.230 kb on + strand, within GFF2121 at 170.230 kb on + strand, within GFF2121 at 170.283 kb on - strand, within GFF2121 at 170.297 kb on - strand, within GFF2121 at 170.336 kb on - strand, within GFF2121 at 170.363 kb on - strand, within GFF2121 at 170.384 kb on - strand, within GFF2121 at 170.550 kb on - strand, within GFF2121 at 170.614 kb on + strand, within GFF2121 at 170.614 kb on + strand, within GFF2121 at 170.614 kb on + strand, within GFF2121 at 170.614 kb on + strand, within GFF2121 at 170.615 kb on - strand, within GFF2121 at 170.746 kb on + strand, within GFF2121 at 170.746 kb on + strand, within GFF2121 at 170.746 kb on + strand, within GFF2121 at 170.747 kb on - strand, within GFF2121 at 170.747 kb on - strand, within GFF2121 at 170.747 kb on - strand, within GFF2121
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 168,245 + GFF2118 0.61 +0.4 168,290 - GFF2118 0.69 +0.0 168,293 + GFF2118 0.70 +0.4 168,371 + GFF2118 0.84 +0.0 168,372 - GFF2118 0.84 -0.5 168,447 - +0.3 168,829 + GFF2119 0.49 -1.5 168,829 + GFF2119 0.49 +0.7 168,915 + GFF2119 0.60 +0.1 168,916 - GFF2119 0.60 -1.9 168,916 - GFF2119 0.60 +1.1 168,916 - GFF2119 0.60 +0.1 169,027 + GFF2119 0.75 -0.1 169,027 + GFF2119 0.75 -1.3 169,027 + GFF2119 0.75 +0.2 169,028 - GFF2119 0.75 -1.3 169,028 - GFF2119 0.75 -0.6 169,028 - GFF2119 0.75 +0.2 169,028 - GFF2119 0.75 +0.1 169,028 - GFF2119 0.75 +0.4 169,028 - GFF2119 0.75 -0.1 169,028 - GFF2119 0.75 -0.8 169,235 + -2.2 169,338 + GFF2120 0.21 -0.4 169,339 - GFF2120 0.21 -0.4 169,339 - GFF2120 0.21 -0.3 169,339 - GFF2120 0.21 +0.0 169,339 - GFF2120 0.21 +1.3 169,507 + GFF2120 0.50 +0.6 169,563 + GFF2120 0.59 +0.6 169,629 + GFF2120 0.71 +0.7 169,630 - GFF2120 0.71 +1.1 169,633 - GFF2120 0.71 +1.5 169,660 - GFF2120 0.76 -0.9 169,758 + +0.1 169,759 - +0.6 169,942 + -0.7 169,942 + -0.2 169,943 - +0.4 170,029 + -0.7 170,029 + -0.7 170,029 + -0.6 170,030 - -0.7 170,033 - +0.5 170,033 - +0.8 170,033 - +0.5 170,126 + +1.4 170,198 + GFF2121 0.11 -0.6 170,230 + GFF2121 0.12 -2.9 170,230 + GFF2121 0.12 +0.5 170,283 - GFF2121 0.13 -0.5 170,297 - GFF2121 0.14 -0.1 170,336 - GFF2121 0.15 -0.2 170,363 - GFF2121 0.16 +0.1 170,384 - GFF2121 0.16 -0.2 170,550 - GFF2121 0.21 -0.1 170,614 + GFF2121 0.23 -1.9 170,614 + GFF2121 0.23 +0.0 170,614 + GFF2121 0.23 +0.1 170,614 + GFF2121 0.23 +0.2 170,615 - GFF2121 0.23 -0.3 170,746 + GFF2121 0.27 -0.9 170,746 + GFF2121 0.27 +0.4 170,746 + GFF2121 0.27 +1.3 170,747 - GFF2121 0.27 +0.0 170,747 - GFF2121 0.27 -0.4 170,747 - GFF2121 0.27 +0.7
Or see this region's nucleotide sequence