Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2071 and GFF2072 are separated by 1 nucleotides GFF2072 and GFF2073 are separated by 20 nucleotides GFF2073 and GFF2074 overlap by 4 nucleotides GFF2074 and GFF2075 are separated by 4 nucleotides
GFF2071 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 118,259 to 118,726
GFF2071
GFF2072 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 118,728 to 119,684
GFF2072
GFF2073 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 119,705 to 120,277
GFF2073
GFF2074 - hypothetical protein, at 120,274 to 121,161
GFF2074
GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161
GFF2075
Position (kb) 119
120
121 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.762 kb on + strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 118.763 kb on - strand at 119.095 kb on + strand, within GFF2072 at 119.095 kb on + strand, within GFF2072 at 119.096 kb on - strand, within GFF2072 at 119.096 kb on - strand, within GFF2072 at 119.110 kb on + strand, within GFF2072 at 119.110 kb on + strand, within GFF2072 at 119.110 kb on + strand, within GFF2072 at 119.111 kb on - strand, within GFF2072 at 119.122 kb on + strand, within GFF2072 at 119.122 kb on + strand, within GFF2072 at 119.122 kb on + strand, within GFF2072 at 119.123 kb on - strand, within GFF2072 at 119.123 kb on - strand, within GFF2072 at 119.272 kb on + strand, within GFF2072 at 119.272 kb on + strand, within GFF2072 at 119.272 kb on + strand, within GFF2072 at 119.272 kb on + strand, within GFF2072 at 119.273 kb on - strand, within GFF2072 at 119.273 kb on - strand, within GFF2072 at 119.273 kb on - strand, within GFF2072 at 119.273 kb on - strand, within GFF2072 at 119.338 kb on + strand, within GFF2072 at 119.338 kb on + strand, within GFF2072 at 119.338 kb on + strand, within GFF2072 at 119.339 kb on - strand, within GFF2072 at 119.339 kb on - strand, within GFF2072 at 119.485 kb on + strand, within GFF2072 at 119.485 kb on + strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.486 kb on - strand, within GFF2072 at 119.709 kb on + strand at 119.780 kb on + strand, within GFF2073 at 119.780 kb on + strand, within GFF2073 at 119.781 kb on - strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.810 kb on + strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 119.811 kb on - strand, within GFF2073 at 120.128 kb on - strand, within GFF2073 at 120.388 kb on + strand, within GFF2074 at 120.409 kb on + strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 120.410 kb on - strand, within GFF2074 at 121.054 kb on + strand, within GFF2074 at 121.055 kb on - strand, within GFF2074
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 118,762 + -0.4 118,762 + -0.6 118,762 + -0.1 118,762 + -0.5 118,762 + -0.6 118,763 - -0.3 118,763 - +0.3 118,763 - -0.2 118,763 - +1.0 118,763 - -2.5 118,763 - -0.8 118,763 - -0.8 118,763 - -0.7 119,095 + GFF2072 0.38 +0.4 119,095 + GFF2072 0.38 +0.7 119,096 - GFF2072 0.38 -0.7 119,096 - GFF2072 0.38 +0.6 119,110 + GFF2072 0.40 -0.6 119,110 + GFF2072 0.40 +0.0 119,110 + GFF2072 0.40 +0.0 119,111 - GFF2072 0.40 -0.0 119,122 + GFF2072 0.41 +1.6 119,122 + GFF2072 0.41 +0.8 119,122 + GFF2072 0.41 +0.2 119,123 - GFF2072 0.41 -0.2 119,123 - GFF2072 0.41 -0.2 119,272 + GFF2072 0.57 +0.3 119,272 + GFF2072 0.57 -0.5 119,272 + GFF2072 0.57 +0.1 119,272 + GFF2072 0.57 -0.4 119,273 - GFF2072 0.57 +0.0 119,273 - GFF2072 0.57 +0.2 119,273 - GFF2072 0.57 -1.9 119,273 - GFF2072 0.57 +0.5 119,338 + GFF2072 0.64 -0.0 119,338 + GFF2072 0.64 -0.6 119,338 + GFF2072 0.64 -0.3 119,339 - GFF2072 0.64 -0.3 119,339 - GFF2072 0.64 -0.2 119,485 + GFF2072 0.79 -1.3 119,485 + GFF2072 0.79 -0.2 119,486 - GFF2072 0.79 +0.0 119,486 - GFF2072 0.79 -0.2 119,486 - GFF2072 0.79 -0.3 119,486 - GFF2072 0.79 +0.3 119,709 + -1.4 119,780 + GFF2073 0.13 +0.2 119,780 + GFF2073 0.13 -0.1 119,781 - GFF2073 0.13 -2.4 119,810 + GFF2073 0.18 -3.4 119,810 + GFF2073 0.18 -0.4 119,810 + GFF2073 0.18 -2.5 119,810 + GFF2073 0.18 -0.3 119,811 - GFF2073 0.18 -0.8 119,811 - GFF2073 0.18 -0.6 119,811 - GFF2073 0.18 -2.2 119,811 - GFF2073 0.18 -1.1 119,811 - GFF2073 0.18 -1.9 119,811 - GFF2073 0.18 -1.1 120,128 - GFF2073 0.74 +0.6 120,388 + GFF2074 0.13 -0.6 120,409 + GFF2074 0.15 +0.4 120,410 - GFF2074 0.15 +0.1 120,410 - GFF2074 0.15 +0.2 121,054 + GFF2074 0.88 +1.1 121,055 - GFF2074 0.88 +0.3
Or see this region's nucleotide sequence