Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF206 and GFF207 are separated by 11 nucleotides GFF207 and GFF208 overlap by 4 nucleotides GFF208 and GFF209 are separated by 77 nucleotides
GFF206 - GMP synthase [glutamine-hydrolyzing], amidotransferase subunit (EC 6.3.5.2) / GMP synthase [glutamine-hydrolyzing], ATP pyrophosphatase subunit (EC 6.3.5.2), at 233,157 to 234,773
GFF206
GFF207 - Death on curing protein, Doc toxin, at 234,785 to 235,219
GFF207
GFF208 - Prevent host death protein, Phd antitoxin, at 235,216 to 235,464
GFF208
GFF209 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 235,542 to 237,011
GFF209
Position (kb) 234
235
236 Strain fitness (log2 ratio) -2
-1
0
1
2 at 234.894 kb on + strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.895 kb on - strand, within GFF207 at 234.906 kb on + strand, within GFF207 at 234.907 kb on - strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.137 kb on + strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.138 kb on - strand, within GFF207 at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.456 kb on - strand at 235.498 kb on + strand at 235.543 kb on + strand at 235.543 kb on + strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.544 kb on - strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.555 kb on + strand at 235.556 kb on - strand at 235.556 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 234,894 + GFF207 0.25 +0.7 234,895 - GFF207 0.25 +2.0 234,895 - GFF207 0.25 +0.1 234,906 + GFF207 0.28 -1.6 234,907 - GFF207 0.28 -0.8 235,137 + GFF207 0.81 +0.9 235,137 + GFF207 0.81 -0.6 235,137 + GFF207 0.81 -1.3 235,137 + GFF207 0.81 -1.5 235,138 - GFF207 0.81 +1.2 235,138 - GFF207 0.81 -0.7 235,456 - +0.2 235,456 - -0.9 235,456 - -1.2 235,456 - -0.8 235,456 - +1.2 235,456 - -0.1 235,456 - -0.8 235,456 - -0.4 235,498 + +0.3 235,543 + -0.3 235,543 + +0.2 235,544 - -0.4 235,544 - +0.3 235,544 - +0.7 235,544 - +0.9 235,555 + +0.1 235,555 + -1.8 235,555 + -2.6 235,555 + -0.9 235,556 - -0.5 235,556 - +0.0
Or see this region's nucleotide sequence