Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1962 and GFF1963 are separated by 145 nucleotides GFF1963 and GFF1964 are separated by 216 nucleotides GFF1964 and GFF1965 are separated by 188 nucleotides
GFF1962 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 4,576 to 5,703
GFF1962
GFF1963 - Transcriptional regulator, AsnC family, at 5,849 to 6,322
GFF1963
GFF1964 - Histone acetyltransferase HPA2 and related acetyltransferases, at 6,539 to 7,159
GFF1964
GFF1965 - Magnesium and cobalt efflux protein CorC, at 7,348 to 8,226
GFF1965
Position (kb) 6
7
8 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5.627 kb on + strand at 5.627 kb on + strand at 5.627 kb on + strand at 5.628 kb on - strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.768 kb on + strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.769 kb on - strand at 5.872 kb on + strand at 5.872 kb on + strand at 5.873 kb on - strand at 5.873 kb on - strand at 5.873 kb on - strand at 5.873 kb on - strand at 5.915 kb on + strand, within GFF1963 at 5.915 kb on + strand, within GFF1963 at 5.915 kb on + strand, within GFF1963 at 5.915 kb on + strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 5.916 kb on - strand, within GFF1963 at 6.005 kb on + strand, within GFF1963 at 6.005 kb on + strand, within GFF1963 at 6.005 kb on + strand, within GFF1963 at 6.005 kb on + strand, within GFF1963 at 6.005 kb on + strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.006 kb on - strand, within GFF1963 at 6.053 kb on + strand, within GFF1963 at 6.053 kb on + strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.054 kb on - strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.179 kb on + strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.180 kb on - strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.215 kb on + strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.216 kb on - strand, within GFF1963 at 6.321 kb on - strand at 6.321 kb on - strand at 6.405 kb on + strand at 6.419 kb on + strand at 6.420 kb on - strand at 6.420 kb on - strand at 6.518 kb on - strand at 6.518 kb on - strand at 6.620 kb on + strand, within GFF1964 at 6.620 kb on + strand, within GFF1964 at 6.621 kb on - strand, within GFF1964 at 6.621 kb on - strand, within GFF1964 at 6.621 kb on - strand, within GFF1964 at 6.636 kb on - strand, within GFF1964 at 6.665 kb on + strand, within GFF1964 at 6.665 kb on + strand, within GFF1964 at 6.665 kb on + strand, within GFF1964 at 6.665 kb on + strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.666 kb on - strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.710 kb on + strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.711 kb on - strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.879 kb on + strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 6.880 kb on - strand, within GFF1964 at 7.043 kb on + strand, within GFF1964 at 7.043 kb on + strand, within GFF1964 at 7.043 kb on + strand, within GFF1964 at 7.043 kb on + strand, within GFF1964 at 7.043 kb on + strand, within GFF1964 at 7.044 kb on - strand, within GFF1964 at 7.044 kb on - strand, within GFF1964 at 7.044 kb on - strand, within GFF1964 at 7.144 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.157 kb on + strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.158 kb on - strand at 7.198 kb on + strand at 7.198 kb on + strand at 7.198 kb on + strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.199 kb on - strand at 7.268 kb on + strand at 7.269 kb on - strand at 7.269 kb on - strand at 7.269 kb on - strand at 7.285 kb on + strand at 7.285 kb on + strand at 7.286 kb on - strand at 7.286 kb on - strand at 7.286 kb on - strand at 7.286 kb on - strand at 7.663 kb on + strand, within GFF1965 at 7.663 kb on + strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.664 kb on - strand, within GFF1965 at 7.978 kb on + strand, within GFF1965 at 7.978 kb on + strand, within GFF1965 at 7.978 kb on + strand, within GFF1965 at 7.978 kb on + strand, within GFF1965 at 7.978 kb on + strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965 at 7.979 kb on - strand, within GFF1965
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 5,627 + -0.2 5,627 + +0.0 5,627 + +0.0 5,628 - -0.1 5,768 + -0.1 5,768 + -0.3 5,768 + -0.5 5,768 + +0.6 5,768 + -0.1 5,768 + -0.6 5,768 + -0.1 5,768 + -0.1 5,768 + -0.4 5,769 - +0.1 5,769 - -0.1 5,769 - +1.1 5,769 - +0.4 5,769 - +0.2 5,769 - -1.9 5,769 - -0.3 5,769 - +0.7 5,769 - +1.0 5,769 - -0.5 5,769 - -2.6 5,769 - -0.2 5,872 + -0.9 5,872 + +1.0 5,873 - -0.0 5,873 - -0.5 5,873 - +1.7 5,873 - -0.5 5,915 + GFF1963 0.14 -0.5 5,915 + GFF1963 0.14 +0.2 5,915 + GFF1963 0.14 -1.2 5,915 + GFF1963 0.14 -0.8 5,916 - GFF1963 0.14 -0.9 5,916 - GFF1963 0.14 -0.5 5,916 - GFF1963 0.14 -0.5 5,916 - GFF1963 0.14 -0.5 5,916 - GFF1963 0.14 -0.9 5,916 - GFF1963 0.14 +0.4 5,916 - GFF1963 0.14 -1.1 5,916 - GFF1963 0.14 +0.2 5,916 - GFF1963 0.14 +0.4 5,916 - GFF1963 0.14 -0.1 6,005 + GFF1963 0.33 -0.7 6,005 + GFF1963 0.33 +0.2 6,005 + GFF1963 0.33 +0.7 6,005 + GFF1963 0.33 +0.1 6,005 + GFF1963 0.33 -0.9 6,006 - GFF1963 0.33 -1.2 6,006 - GFF1963 0.33 +0.4 6,006 - GFF1963 0.33 +0.5 6,006 - GFF1963 0.33 -0.3 6,006 - GFF1963 0.33 -0.7 6,006 - GFF1963 0.33 -0.4 6,006 - GFF1963 0.33 -0.8 6,006 - GFF1963 0.33 -2.1 6,006 - GFF1963 0.33 -0.5 6,006 - GFF1963 0.33 -0.6 6,006 - GFF1963 0.33 +0.5 6,006 - GFF1963 0.33 -0.7 6,006 - GFF1963 0.33 -0.3 6,006 - GFF1963 0.33 -0.1 6,006 - GFF1963 0.33 -0.6 6,006 - GFF1963 0.33 -0.5 6,006 - GFF1963 0.33 -1.7 6,053 + GFF1963 0.43 -1.0 6,053 + GFF1963 0.43 -1.3 6,054 - GFF1963 0.43 -1.3 6,054 - GFF1963 0.43 +0.1 6,054 - GFF1963 0.43 -0.5 6,054 - GFF1963 0.43 -0.0 6,054 - GFF1963 0.43 -0.3 6,054 - GFF1963 0.43 +0.1 6,054 - GFF1963 0.43 -0.3 6,179 + GFF1963 0.70 +0.0 6,179 + GFF1963 0.70 -0.9 6,179 + GFF1963 0.70 -0.3 6,179 + GFF1963 0.70 +0.7 6,179 + GFF1963 0.70 +0.3 6,179 + GFF1963 0.70 -0.4 6,179 + GFF1963 0.70 -0.6 6,179 + GFF1963 0.70 -0.1 6,179 + GFF1963 0.70 -0.1 6,180 - GFF1963 0.70 -0.8 6,180 - GFF1963 0.70 -0.5 6,180 - GFF1963 0.70 +0.6 6,180 - GFF1963 0.70 -0.2 6,180 - GFF1963 0.70 +0.7 6,180 - GFF1963 0.70 -0.1 6,180 - GFF1963 0.70 -0.1 6,180 - GFF1963 0.70 -0.8 6,180 - GFF1963 0.70 +0.2 6,180 - GFF1963 0.70 -0.1 6,180 - GFF1963 0.70 +1.3 6,180 - GFF1963 0.70 -1.1 6,180 - GFF1963 0.70 +0.2 6,180 - GFF1963 0.70 +0.2 6,180 - GFF1963 0.70 -0.7 6,180 - GFF1963 0.70 -0.6 6,215 + GFF1963 0.77 +0.5 6,215 + GFF1963 0.77 -1.3 6,215 + GFF1963 0.77 -0.1 6,215 + GFF1963 0.77 -0.1 6,215 + GFF1963 0.77 -1.9 6,215 + GFF1963 0.77 -0.7 6,215 + GFF1963 0.77 -0.6 6,215 + GFF1963 0.77 -0.0 6,215 + GFF1963 0.77 +0.7 6,215 + GFF1963 0.77 +0.2 6,215 + GFF1963 0.77 +0.5 6,216 - GFF1963 0.77 -1.2 6,216 - GFF1963 0.77 +1.3 6,216 - GFF1963 0.77 -1.9 6,216 - GFF1963 0.77 +0.7 6,216 - GFF1963 0.77 +0.3 6,216 - GFF1963 0.77 -0.7 6,216 - GFF1963 0.77 +0.4 6,216 - GFF1963 0.77 -1.4 6,216 - GFF1963 0.77 -1.9 6,321 - +0.4 6,321 - +0.6 6,405 + +1.0 6,419 + -0.3 6,420 - +0.1 6,420 - -0.3 6,518 - -1.6 6,518 - -0.1 6,620 + GFF1964 0.13 -0.6 6,620 + GFF1964 0.13 +0.2 6,621 - GFF1964 0.13 -0.8 6,621 - GFF1964 0.13 -0.3 6,621 - GFF1964 0.13 -0.3 6,636 - GFF1964 0.16 -0.3 6,665 + GFF1964 0.20 +0.4 6,665 + GFF1964 0.20 +0.2 6,665 + GFF1964 0.20 -0.6 6,665 + GFF1964 0.20 -0.9 6,666 - GFF1964 0.20 +0.7 6,666 - GFF1964 0.20 -0.2 6,666 - GFF1964 0.20 -0.6 6,666 - GFF1964 0.20 +1.3 6,666 - GFF1964 0.20 +0.7 6,666 - GFF1964 0.20 +0.3 6,710 + GFF1964 0.28 +0.5 6,710 + GFF1964 0.28 +0.1 6,710 + GFF1964 0.28 -0.6 6,710 + GFF1964 0.28 +0.3 6,710 + GFF1964 0.28 -2.1 6,710 + GFF1964 0.28 +0.7 6,710 + GFF1964 0.28 -1.0 6,710 + GFF1964 0.28 -0.1 6,710 + GFF1964 0.28 -0.3 6,710 + GFF1964 0.28 -1.7 6,710 + GFF1964 0.28 -0.3 6,710 + GFF1964 0.28 -0.5 6,710 + GFF1964 0.28 +0.1 6,710 + GFF1964 0.28 -1.5 6,710 + GFF1964 0.28 -0.4 6,710 + GFF1964 0.28 +1.1 6,710 + GFF1964 0.28 -0.2 6,710 + GFF1964 0.28 -0.4 6,710 + GFF1964 0.28 +0.3 6,711 - GFF1964 0.28 -0.8 6,711 - GFF1964 0.28 +0.6 6,711 - GFF1964 0.28 +0.1 6,711 - GFF1964 0.28 +0.1 6,711 - GFF1964 0.28 -1.3 6,711 - GFF1964 0.28 -0.4 6,711 - GFF1964 0.28 +0.1 6,711 - GFF1964 0.28 -0.6 6,711 - GFF1964 0.28 +1.3 6,711 - GFF1964 0.28 -0.5 6,711 - GFF1964 0.28 -0.0 6,879 + GFF1964 0.55 +0.4 6,879 + GFF1964 0.55 +0.0 6,879 + GFF1964 0.55 +1.7 6,879 + GFF1964 0.55 -0.1 6,879 + GFF1964 0.55 -0.1 6,879 + GFF1964 0.55 -0.5 6,879 + GFF1964 0.55 -0.4 6,879 + GFF1964 0.55 +0.1 6,879 + GFF1964 0.55 -0.4 6,880 - GFF1964 0.55 -0.2 6,880 - GFF1964 0.55 -0.1 6,880 - GFF1964 0.55 +0.6 6,880 - GFF1964 0.55 +0.2 6,880 - GFF1964 0.55 -0.6 6,880 - GFF1964 0.55 -0.3 6,880 - GFF1964 0.55 +0.8 6,880 - GFF1964 0.55 +0.5 7,043 + GFF1964 0.81 -1.2 7,043 + GFF1964 0.81 -0.2 7,043 + GFF1964 0.81 -0.7 7,043 + GFF1964 0.81 +0.7 7,043 + GFF1964 0.81 -0.3 7,044 - GFF1964 0.81 -0.9 7,044 - GFF1964 0.81 +1.0 7,044 - GFF1964 0.81 +1.9 7,144 + +0.2 7,157 + -0.1 7,157 + -0.5 7,157 + +0.6 7,157 + -1.3 7,157 + -0.7 7,157 + +0.0 7,157 + +0.4 7,157 + -0.2 7,157 + +0.7 7,157 + -1.1 7,157 + -0.7 7,158 - -0.2 7,158 - -0.0 7,158 - -0.6 7,158 - +1.1 7,158 - -1.9 7,158 - -0.0 7,158 - -0.4 7,158 - -0.5 7,158 - -0.1 7,198 + +0.6 7,198 + +3.3 7,198 + -0.6 7,199 - -0.3 7,199 - -0.5 7,199 - -0.3 7,199 - -1.5 7,199 - -0.3 7,199 - +0.5 7,199 - -0.8 7,199 - +0.4 7,268 + +1.7 7,269 - -0.0 7,269 - +0.1 7,269 - -0.5 7,285 + +1.2 7,285 + +0.1 7,286 - +0.6 7,286 - -0.5 7,286 - +0.4 7,286 - -1.4 7,663 + GFF1965 0.36 -0.3 7,663 + GFF1965 0.36 +0.3 7,664 - GFF1965 0.36 +0.7 7,664 - GFF1965 0.36 -0.5 7,664 - GFF1965 0.36 +0.3 7,664 - GFF1965 0.36 +1.1 7,664 - GFF1965 0.36 -1.0 7,664 - GFF1965 0.36 +0.5 7,664 - GFF1965 0.36 -0.6 7,664 - GFF1965 0.36 -0.5 7,664 - GFF1965 0.36 -1.8 7,664 - GFF1965 0.36 +1.3 7,978 + GFF1965 0.72 -2.0 7,978 + GFF1965 0.72 -0.5 7,978 + GFF1965 0.72 +0.3 7,978 + GFF1965 0.72 -1.5 7,978 + GFF1965 0.72 +0.1 7,979 - GFF1965 0.72 +1.2 7,979 - GFF1965 0.72 +0.4 7,979 - GFF1965 0.72 -0.4 7,979 - GFF1965 0.72 +1.4 7,979 - GFF1965 0.72 -1.6 7,979 - GFF1965 0.72 +1.0 7,979 - GFF1965 0.72 -1.8 7,979 - GFF1965 0.72 +2.2 7,979 - GFF1965 0.72 -1.5 7,979 - GFF1965 0.72 -0.4 7,979 - GFF1965 0.72 +0.2 7,979 - GFF1965 0.72 +0.5
Or see this region's nucleotide sequence