Strain Fitness in Variovorax sp. SCN45 around GFF1858

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1857 and GFF1858 overlap by 4 nucleotidesGFF1858 and GFF1859 overlap by 4 nucleotidesGFF1859 and GFF1860 are separated by 12 nucleotides GFF1857 - FIG00553873: hypothetical protein, at 531,838 to 533,118 GFF1857 GFF1858 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 533,115 to 533,879 GFF1858 GFF1859 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 533,876 to 534,547 GFF1859 GFF1860 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 534,560 to 535,327 GFF1860 Position (kb) 533 534Strain fitness (log2 ratio) -2 -1 0 1 2at 532.116 kb on - strand, within GFF1857at 532.250 kb on + strand, within GFF1857at 532.251 kb on - strand, within GFF1857at 532.251 kb on - strand, within GFF1857at 532.360 kb on + strand, within GFF1857at 532.360 kb on + strand, within GFF1857at 532.361 kb on - strand, within GFF1857at 532.398 kb on - strand, within GFF1857at 532.508 kb on + strand, within GFF1857at 532.508 kb on + strand, within GFF1857at 532.508 kb on + strand, within GFF1857at 532.509 kb on - strand, within GFF1857at 532.509 kb on - strand, within GFF1857at 533.722 kb on + strand, within GFF1858at 533.928 kb on + strandat 533.928 kb on + strandat 533.928 kb on + strandat 533.928 kb on + strandat 533.929 kb on - strandat 533.929 kb on - strandat 533.929 kb on - strandat 533.955 kb on + strand, within GFF1859at 533.955 kb on + strand, within GFF1859at 533.955 kb on + strand, within GFF1859at 533.956 kb on - strand, within GFF1859at 533.956 kb on - strand, within GFF1859at 533.956 kb on - strand, within GFF1859at 533.956 kb on - strand, within GFF1859at 533.976 kb on + strand, within GFF1859at 533.976 kb on + strand, within GFF1859at 533.977 kb on - strand, within GFF1859at 534.097 kb on - strand, within GFF1859at 534.097 kb on - strand, within GFF1859at 534.132 kb on - strand, within GFF1859at 534.132 kb on - strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.318 kb on + strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.319 kb on - strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.324 kb on + strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.325 kb on - strand, within GFF1859at 534.699 kb on + strand, within GFF1860at 534.699 kb on + strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.700 kb on - strand, within GFF1860at 534.747 kb on + strand, within GFF1860at 534.747 kb on + strand, within GFF1860at 534.748 kb on - strand, within GFF1860at 534.748 kb on - strand, within GFF1860at 534.828 kb on + strand, within GFF1860at 534.828 kb on + strand, within GFF1860at 534.828 kb on + strand, within GFF1860at 534.828 kb on + strand, within GFF1860at 534.828 kb on + strand, within GFF1860at 534.829 kb on - strand, within GFF1860at 534.829 kb on - strand, within GFF1860

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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532,116 - GFF1857 0.22 -0.9
532,250 + GFF1857 0.32 +1.7
532,251 - GFF1857 0.32 -0.1
532,251 - GFF1857 0.32 -0.1
532,360 + GFF1857 0.41 +0.1
532,360 + GFF1857 0.41 +1.2
532,361 - GFF1857 0.41 -0.5
532,398 - GFF1857 0.44 +0.2
532,508 + GFF1857 0.52 -1.7
532,508 + GFF1857 0.52 -0.1
532,508 + GFF1857 0.52 +0.3
532,509 - GFF1857 0.52 +0.5
532,509 - GFF1857 0.52 -0.6
533,722 + GFF1858 0.79 -0.9
533,928 + -0.6
533,928 + +0.5
533,928 + -0.0
533,928 + -1.2
533,929 - -0.6
533,929 - +2.2
533,929 - -1.7
533,955 + GFF1859 0.12 -2.0
533,955 + GFF1859 0.12 -0.3
533,955 + GFF1859 0.12 -0.7
533,956 - GFF1859 0.12 -0.5
533,956 - GFF1859 0.12 -1.7
533,956 - GFF1859 0.12 -0.6
533,956 - GFF1859 0.12 -1.1
533,976 + GFF1859 0.15 -0.6
533,976 + GFF1859 0.15 -0.4
533,977 - GFF1859 0.15 +0.1
534,097 - GFF1859 0.33 +0.9
534,097 - GFF1859 0.33 -0.3
534,132 - GFF1859 0.38 -0.0
534,132 - GFF1859 0.38 -0.1
534,318 + GFF1859 0.66 +0.9
534,318 + GFF1859 0.66 -0.5
534,318 + GFF1859 0.66 +1.1
534,318 + GFF1859 0.66 -0.5
534,318 + GFF1859 0.66 -2.3
534,318 + GFF1859 0.66 +0.0
534,319 - GFF1859 0.66 -0.1
534,319 - GFF1859 0.66 +0.3
534,319 - GFF1859 0.66 -0.3
534,319 - GFF1859 0.66 +1.1
534,319 - GFF1859 0.66 -1.7
534,319 - GFF1859 0.66 -1.7
534,324 + GFF1859 0.67 +0.0
534,324 + GFF1859 0.67 +0.8
534,324 + GFF1859 0.67 -1.0
534,324 + GFF1859 0.67 -1.4
534,324 + GFF1859 0.67 +0.5
534,324 + GFF1859 0.67 +0.7
534,324 + GFF1859 0.67 -1.3
534,324 + GFF1859 0.67 +0.3
534,325 - GFF1859 0.67 -0.5
534,325 - GFF1859 0.67 -0.2
534,325 - GFF1859 0.67 +1.9
534,325 - GFF1859 0.67 -1.8
534,325 - GFF1859 0.67 +0.0
534,325 - GFF1859 0.67 -0.2
534,325 - GFF1859 0.67 -0.3
534,699 + GFF1860 0.18 +0.4
534,699 + GFF1860 0.18 -0.9
534,700 - GFF1860 0.18 -1.1
534,700 - GFF1860 0.18 -0.5
534,700 - GFF1860 0.18 +0.3
534,700 - GFF1860 0.18 +0.1
534,700 - GFF1860 0.18 -0.9
534,700 - GFF1860 0.18 +0.0
534,700 - GFF1860 0.18 -0.9
534,700 - GFF1860 0.18 -0.5
534,700 - GFF1860 0.18 -0.5
534,747 + GFF1860 0.24 -0.9
534,747 + GFF1860 0.24 +1.0
534,748 - GFF1860 0.24 +0.9
534,748 - GFF1860 0.24 -1.2
534,828 + GFF1860 0.35 -1.0
534,828 + GFF1860 0.35 -1.2
534,828 + GFF1860 0.35 +0.8
534,828 + GFF1860 0.35 +0.9
534,828 + GFF1860 0.35 -0.3
534,829 - GFF1860 0.35 -0.7
534,829 - GFF1860 0.35 -0.6

Or see this region's nucleotide sequence