Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1848 and GFF1849 are separated by 18 nucleotides GFF1849 and GFF1850 are separated by 88 nucleotides GFF1850 and GFF1851 overlap by 8 nucleotides
GFF1848 - Proteins incorrectly called adenylate cyclase, at 519,851 to 523,891
GFF1848
GFF1849 - FIG00347667: hypothetical protein, at 523,910 to 524,701
GFF1849
GFF1850 - Tetracenomycin polyketide synthesis O-methyltransferase TcmP, at 524,790 to 525,548
GFF1850
GFF1851 - TOMM biosynthesis cyclodehydratase (protein C) / TOMM biosynthesis docking scaffold (protein D), at 525,541 to 527,778
GFF1851
Position (kb) 524
525
526 Strain fitness (log2 ratio) -1
0
1
2
3 at 523.961 kb on + strand at 523.962 kb on - strand at 523.962 kb on - strand at 524.096 kb on + strand, within GFF1849 at 524.096 kb on + strand, within GFF1849 at 524.231 kb on + strand, within GFF1849 at 524.231 kb on + strand, within GFF1849 at 524.232 kb on - strand, within GFF1849 at 524.232 kb on - strand, within GFF1849 at 524.232 kb on - strand, within GFF1849 at 524.232 kb on - strand, within GFF1849 at 524.234 kb on + strand, within GFF1849 at 524.234 kb on + strand, within GFF1849 at 524.234 kb on + strand, within GFF1849 at 524.234 kb on + strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.235 kb on - strand, within GFF1849 at 524.483 kb on + strand, within GFF1849 at 524.483 kb on + strand, within GFF1849 at 524.483 kb on + strand, within GFF1849 at 524.484 kb on - strand, within GFF1849 at 524.484 kb on - strand, within GFF1849 at 524.612 kb on + strand, within GFF1849 at 524.663 kb on + strand at 524.663 kb on + strand at 524.663 kb on + strand at 524.663 kb on + strand at 524.663 kb on + strand at 524.663 kb on + strand at 524.663 kb on + strand at 524.664 kb on - strand at 524.664 kb on - strand at 524.664 kb on - strand at 524.664 kb on - strand at 524.664 kb on - strand at 524.684 kb on + strand at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.868 kb on + strand, within GFF1850 at 524.869 kb on - strand, within GFF1850 at 524.869 kb on - strand, within GFF1850 at 525.210 kb on + strand, within GFF1850 at 525.210 kb on + strand, within GFF1850 at 525.211 kb on - strand, within GFF1850 at 525.211 kb on - strand, within GFF1850 at 525.416 kb on + strand, within GFF1850 at 525.418 kb on + strand, within GFF1850 at 525.418 kb on + strand, within GFF1850 at 525.435 kb on + strand, within GFF1850 at 525.435 kb on + strand, within GFF1850 at 525.435 kb on + strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.436 kb on - strand, within GFF1850 at 525.504 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.724 kb on + strand at 525.725 kb on - strand at 525.725 kb on - strand at 525.725 kb on - strand at 525.725 kb on - strand at 525.725 kb on - strand at 525.725 kb on - strand at 525.835 kb on + strand, within GFF1851 at 525.835 kb on + strand, within GFF1851 at 525.835 kb on + strand, within GFF1851 at 525.835 kb on + strand, within GFF1851 at 525.835 kb on + strand, within GFF1851 at 525.836 kb on - strand, within GFF1851 at 525.836 kb on - strand, within GFF1851 at 525.836 kb on - strand, within GFF1851 at 525.836 kb on - strand, within GFF1851 at 525.836 kb on - strand, within GFF1851 at 526.027 kb on + strand, within GFF1851 at 526.027 kb on + strand, within GFF1851 at 526.028 kb on - strand, within GFF1851 at 526.028 kb on - strand, within GFF1851 at 526.028 kb on - strand, within GFF1851
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 523,961 + +1.4 523,962 - -0.4 523,962 - -1.5 524,096 + GFF1849 0.23 -0.4 524,096 + GFF1849 0.23 -0.1 524,231 + GFF1849 0.41 -0.8 524,231 + GFF1849 0.41 +0.0 524,232 - GFF1849 0.41 -1.3 524,232 - GFF1849 0.41 -0.5 524,232 - GFF1849 0.41 +1.4 524,232 - GFF1849 0.41 -1.3 524,234 + GFF1849 0.41 +0.5 524,234 + GFF1849 0.41 -0.7 524,234 + GFF1849 0.41 -1.3 524,234 + GFF1849 0.41 +0.5 524,235 - GFF1849 0.41 -0.0 524,235 - GFF1849 0.41 -0.7 524,235 - GFF1849 0.41 -0.4 524,235 - GFF1849 0.41 +0.2 524,235 - GFF1849 0.41 +1.9 524,235 - GFF1849 0.41 -0.7 524,483 + GFF1849 0.72 +1.3 524,483 + GFF1849 0.72 +0.7 524,483 + GFF1849 0.72 +0.6 524,484 - GFF1849 0.72 -0.3 524,484 - GFF1849 0.72 -0.1 524,612 + GFF1849 0.89 +0.4 524,663 + -0.4 524,663 + -0.4 524,663 + +0.5 524,663 + +2.2 524,663 + -0.6 524,663 + -0.3 524,663 + +0.0 524,664 - -0.5 524,664 - -1.5 524,664 - +1.1 524,664 - +0.3 524,664 - -0.2 524,684 + -0.3 524,868 + GFF1850 0.10 -0.5 524,868 + GFF1850 0.10 +0.2 524,868 + GFF1850 0.10 +0.5 524,868 + GFF1850 0.10 +3.0 524,868 + GFF1850 0.10 -0.0 524,868 + GFF1850 0.10 +0.6 524,868 + GFF1850 0.10 +0.1 524,868 + GFF1850 0.10 -1.1 524,869 - GFF1850 0.10 +0.2 524,869 - GFF1850 0.10 +0.4 525,210 + GFF1850 0.55 +0.3 525,210 + GFF1850 0.55 +0.5 525,211 - GFF1850 0.55 +0.4 525,211 - GFF1850 0.55 +0.2 525,416 + GFF1850 0.82 -0.1 525,418 + GFF1850 0.83 -0.2 525,418 + GFF1850 0.83 +0.3 525,435 + GFF1850 0.85 -0.6 525,435 + GFF1850 0.85 -0.1 525,435 + GFF1850 0.85 -0.4 525,436 - GFF1850 0.85 -0.7 525,436 - GFF1850 0.85 +0.7 525,436 - GFF1850 0.85 -0.2 525,436 - GFF1850 0.85 +0.6 525,436 - GFF1850 0.85 +1.2 525,436 - GFF1850 0.85 -0.7 525,504 + +1.2 525,724 + -0.1 525,724 + -1.3 525,724 + -1.3 525,724 + -0.7 525,724 + -0.2 525,724 + +0.2 525,724 + -0.0 525,725 - +0.4 525,725 - -0.4 525,725 - -0.2 525,725 - -0.6 525,725 - -1.5 525,725 - +0.0 525,835 + GFF1851 0.13 -0.1 525,835 + GFF1851 0.13 -0.3 525,835 + GFF1851 0.13 +2.1 525,835 + GFF1851 0.13 -0.1 525,835 + GFF1851 0.13 +0.2 525,836 - GFF1851 0.13 +0.0 525,836 - GFF1851 0.13 +2.4 525,836 - GFF1851 0.13 +1.0 525,836 - GFF1851 0.13 -0.1 525,836 - GFF1851 0.13 -1.5 526,027 + GFF1851 0.22 +0.3 526,027 + GFF1851 0.22 -1.5 526,028 - GFF1851 0.22 -0.8 526,028 - GFF1851 0.22 -0.1 526,028 - GFF1851 0.22 -0.5
Or see this region's nucleotide sequence