Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF184 and GFF185 are separated by 470 nucleotides GFF185 and GFF186 are separated by 27 nucleotides
GFF184 - Adenylate cyclase (EC 4.6.1.1), at 215,462 to 216,349
GFF184
GFF185 - hypothetical protein, at 216,820 to 217,083
GFF185
GFF186 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 217,111 to 218,085
GFF186
GFF187 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.33) / Domain of unknown function, at 218,082 to 219,155
GFF187
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 216.183 kb on + strand, within GFF184 at 216.183 kb on + strand, within GFF184 at 216.184 kb on - strand, within GFF184 at 216.184 kb on - strand, within GFF184 at 216.310 kb on + strand at 216.310 kb on + strand at 216.310 kb on + strand at 216.310 kb on + strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.311 kb on - strand at 216.367 kb on + strand at 216.367 kb on + strand at 216.367 kb on + strand at 216.368 kb on - strand at 216.389 kb on + strand at 216.412 kb on + strand at 216.412 kb on + strand at 216.412 kb on + strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.413 kb on - strand at 216.445 kb on - strand at 216.545 kb on - strand at 216.545 kb on - strand at 216.545 kb on - strand at 216.546 kb on + strand at 216.546 kb on + strand at 216.594 kb on + strand at 216.594 kb on + strand at 216.594 kb on + strand at 216.594 kb on + strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.595 kb on - strand at 216.597 kb on + strand at 216.597 kb on + strand at 216.597 kb on + strand at 216.597 kb on + strand at 216.597 kb on + strand at 216.597 kb on + strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.598 kb on - strand at 216.601 kb on + strand at 216.601 kb on + strand at 216.602 kb on - strand at 216.602 kb on - strand at 216.602 kb on - strand at 216.617 kb on - strand at 216.635 kb on + strand at 216.639 kb on + strand at 216.639 kb on + strand at 216.640 kb on - strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.703 kb on + strand at 216.704 kb on - strand at 216.704 kb on - strand at 216.704 kb on - strand at 216.704 kb on - strand at 216.704 kb on - strand at 216.708 kb on + strand at 216.708 kb on + strand at 216.708 kb on + strand at 216.709 kb on - strand at 216.709 kb on - strand at 216.733 kb on + strand at 216.733 kb on + strand at 216.733 kb on + strand at 216.733 kb on + strand at 216.734 kb on - strand at 216.734 kb on - strand at 216.783 kb on - strand at 216.888 kb on + strand, within GFF185 at 216.931 kb on + strand, within GFF185 at 216.931 kb on + strand, within GFF185 at 216.931 kb on + strand, within GFF185 at 216.932 kb on - strand, within GFF185
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 216,183 + GFF184 0.81 +1.2 216,183 + GFF184 0.81 -0.7 216,184 - GFF184 0.81 -1.8 216,184 - GFF184 0.81 -0.7 216,310 + -0.1 216,310 + +0.1 216,310 + +0.2 216,310 + -0.4 216,311 - -0.7 216,311 - -0.2 216,311 - +0.8 216,311 - -0.4 216,311 - +2.2 216,311 - -0.5 216,311 - +1.2 216,367 + +0.7 216,367 + -3.0 216,367 + -0.5 216,368 - -0.2 216,389 + -1.6 216,412 + +0.0 216,412 + +0.0 216,412 + -0.3 216,413 - +1.2 216,413 - +1.2 216,413 - -0.2 216,413 - +0.1 216,413 - -1.0 216,413 - -0.5 216,413 - +0.1 216,413 - +0.3 216,413 - +0.8 216,445 - -1.1 216,545 - -0.9 216,545 - +0.9 216,545 - -0.8 216,546 + -0.8 216,546 + -0.0 216,594 + -0.6 216,594 + -0.2 216,594 + +0.6 216,594 + -0.3 216,595 - +0.2 216,595 - +3.2 216,595 - -0.8 216,595 - +0.5 216,595 - -1.8 216,595 - -1.8 216,595 - +0.1 216,597 + +0.1 216,597 + -1.6 216,597 + +0.1 216,597 + -0.0 216,597 + -0.6 216,597 + +0.4 216,598 - -0.2 216,598 - -0.6 216,598 - -0.8 216,598 - -1.9 216,598 - -0.9 216,598 - +0.6 216,598 - -0.5 216,601 + -2.0 216,601 + +0.3 216,602 - -0.5 216,602 - -1.3 216,602 - -1.4 216,617 - +0.2 216,635 + -0.3 216,639 + -0.7 216,639 + +1.1 216,640 - +0.2 216,703 + -0.5 216,703 + -0.7 216,703 + +0.4 216,703 + +0.8 216,703 + -0.0 216,703 + +1.2 216,703 + +0.4 216,703 + -0.6 216,703 + -0.4 216,704 - -1.6 216,704 - +0.2 216,704 - -1.7 216,704 - -1.8 216,704 - -0.4 216,708 + +0.8 216,708 + -0.3 216,708 + +0.0 216,709 - +0.2 216,709 - +2.1 216,733 + -0.9 216,733 + +0.4 216,733 + +0.8 216,733 + +0.2 216,734 - -0.8 216,734 - -1.1 216,783 - -1.6 216,888 + GFF185 0.26 -1.2 216,931 + GFF185 0.42 +0.0 216,931 + GFF185 0.42 -2.2 216,931 + GFF185 0.42 -1.7 216,932 - GFF185 0.42 +1.8
Or see this region's nucleotide sequence