Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF167 and GFF168 overlap by 4 nucleotides GFF168 and GFF169 overlap by 4 nucleotides GFF169 and GFF170 overlap by 11 nucleotides
GFF167 - Two-component system sensor histidine kinase, at 195,697 to 199,197
GFF167
GFF168 - MCP methyltransferase, CheR-type, at 199,194 to 200,060
GFF168
GFF169 - Protein-glutamate methylesterase (EC 3.1.1.61), at 200,057 to 200,659
GFF169
GFF170 - Two-component system sensor histidine kinase/response regulator hybrid, at 200,649 to 201,872
GFF170
Position (kb)
199
200
201 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 198.304 kb on + strand, within GFF167 at 198.304 kb on + strand, within GFF167 at 198.619 kb on + strand, within GFF167 at 198.619 kb on + strand, within GFF167 at 198.902 kb on - strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.120 kb on + strand at 199.121 kb on - strand at 199.121 kb on - strand at 199.121 kb on - strand at 199.290 kb on + strand, within GFF168 at 199.290 kb on + strand, within GFF168 at 199.291 kb on - strand, within GFF168 at 199.291 kb on - strand, within GFF168 at 199.291 kb on - strand, within GFF168 at 199.299 kb on + strand, within GFF168 at 199.299 kb on + strand, within GFF168 at 199.300 kb on - strand, within GFF168 at 199.305 kb on + strand, within GFF168 at 199.305 kb on + strand, within GFF168 at 199.305 kb on + strand, within GFF168 at 199.305 kb on + strand, within GFF168 at 199.306 kb on - strand, within GFF168 at 199.306 kb on - strand, within GFF168 at 199.306 kb on - strand, within GFF168 at 199.307 kb on - strand, within GFF168 at 199.320 kb on + strand, within GFF168 at 199.321 kb on - strand, within GFF168 at 199.486 kb on - strand, within GFF168 at 199.531 kb on - strand, within GFF168 at 199.531 kb on - strand, within GFF168 at 199.635 kb on + strand, within GFF168 at 199.635 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.710 kb on + strand, within GFF168 at 199.722 kb on + strand, within GFF168 at 199.723 kb on - strand, within GFF168 at 199.758 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.761 kb on + strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.762 kb on - strand, within GFF168 at 199.773 kb on + strand, within GFF168 at 199.774 kb on - strand, within GFF168 at 199.893 kb on + strand, within GFF168 at 199.894 kb on - strand, within GFF168 at 200.043 kb on + strand at 200.044 kb on - strand at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.175 kb on + strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.176 kb on - strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.303 kb on + strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.304 kb on - strand, within GFF169 at 200.321 kb on + strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.322 kb on - strand, within GFF169 at 200.334 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.471 kb on + strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.472 kb on - strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.526 kb on + strand, within GFF169 at 200.527 kb on - strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.594 kb on + strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.595 kb on - strand, within GFF169 at 200.931 kb on + strand, within GFF170 at 200.931 kb on + strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.932 kb on - strand, within GFF170 at 200.946 kb on + strand, within GFF170 at 200.947 kb on - strand, within GFF170
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 198,304 + GFF167 0.74 -0.1 198,304 + GFF167 0.74 +0.2 198,619 + GFF167 0.83 -0.4 198,619 + GFF167 0.83 -0.1 198,902 - +0.5 199,120 + -0.0 199,120 + -1.2 199,120 + -1.8 199,120 + -1.6 199,120 + +1.2 199,120 + -1.2 199,120 + -0.6 199,121 - -0.8 199,121 - -1.6 199,121 - -0.9 199,290 + GFF168 0.11 -0.8 199,290 + GFF168 0.11 -1.1 199,291 - GFF168 0.11 -0.8 199,291 - GFF168 0.11 -1.9 199,291 - GFF168 0.11 -2.2 199,299 + GFF168 0.12 -1.6 199,299 + GFF168 0.12 +0.2 199,300 - GFF168 0.12 -3.6 199,305 + GFF168 0.13 -1.8 199,305 + GFF168 0.13 -0.6 199,305 + GFF168 0.13 +0.2 199,305 + GFF168 0.13 -0.8 199,306 - GFF168 0.13 -1.1 199,306 - GFF168 0.13 -1.8 199,306 - GFF168 0.13 -2.2 199,307 - GFF168 0.13 -2.9 199,320 + GFF168 0.15 -0.6 199,321 - GFF168 0.15 -3.7 199,486 - GFF168 0.34 -2.3 199,531 - GFF168 0.39 -1.8 199,531 - GFF168 0.39 -2.3 199,635 + GFF168 0.51 -0.6 199,635 + GFF168 0.51 +0.2 199,710 + GFF168 0.60 -1.6 199,710 + GFF168 0.60 -0.6 199,710 + GFF168 0.60 -4.3 199,722 + GFF168 0.61 -1.1 199,723 - GFF168 0.61 -3.9 199,758 + GFF168 0.65 -2.6 199,761 + GFF168 0.65 -3.1 199,761 + GFF168 0.65 -2.3 199,761 + GFF168 0.65 +0.9 199,761 + GFF168 0.65 +0.4 199,761 + GFF168 0.65 -1.8 199,761 + GFF168 0.65 -3.3 199,761 + GFF168 0.65 -0.9 199,761 + GFF168 0.65 +1.2 199,762 - GFF168 0.66 -2.1 199,762 - GFF168 0.66 -1.6 199,773 + GFF168 0.67 -1.2 199,774 - GFF168 0.67 -1.8 199,893 + GFF168 0.81 -0.8 199,894 - GFF168 0.81 -1.8 200,043 + -1.9 200,044 - -1.7 200,175 + GFF169 0.20 -1.0 200,175 + GFF169 0.20 -0.7 200,175 + GFF169 0.20 -0.9 200,175 + GFF169 0.20 -0.9 200,175 + GFF169 0.20 +0.5 200,175 + GFF169 0.20 -0.4 200,176 - GFF169 0.20 -0.7 200,176 - GFF169 0.20 -1.7 200,176 - GFF169 0.20 -1.3 200,303 + GFF169 0.41 -1.4 200,303 + GFF169 0.41 -0.6 200,303 + GFF169 0.41 -1.1 200,303 + GFF169 0.41 +0.8 200,304 - GFF169 0.41 -1.7 200,304 - GFF169 0.41 -1.3 200,304 - GFF169 0.41 -0.6 200,321 + GFF169 0.44 -0.8 200,322 - GFF169 0.44 -0.7 200,322 - GFF169 0.44 -1.0 200,322 - GFF169 0.44 -0.8 200,334 + GFF169 0.46 -0.1 200,471 + GFF169 0.69 -0.9 200,471 + GFF169 0.69 -0.7 200,471 + GFF169 0.69 -0.3 200,471 + GFF169 0.69 -1.5 200,471 + GFF169 0.69 -1.3 200,471 + GFF169 0.69 -0.9 200,471 + GFF169 0.69 -1.8 200,471 + GFF169 0.69 -1.3 200,472 - GFF169 0.69 -0.3 200,472 - GFF169 0.69 -0.1 200,472 - GFF169 0.69 +0.7 200,526 + GFF169 0.78 -0.7 200,526 + GFF169 0.78 -1.1 200,526 + GFF169 0.78 -0.6 200,527 - GFF169 0.78 -0.3 200,594 + GFF169 0.89 -0.2 200,594 + GFF169 0.89 -0.4 200,594 + GFF169 0.89 -1.9 200,594 + GFF169 0.89 -1.7 200,595 - GFF169 0.89 -1.1 200,595 - GFF169 0.89 -1.6 200,595 - GFF169 0.89 -1.0 200,595 - GFF169 0.89 -0.8 200,931 + GFF170 0.23 -0.2 200,931 + GFF170 0.23 -0.7 200,932 - GFF170 0.23 -0.3 200,932 - GFF170 0.23 -0.8 200,932 - GFF170 0.23 +3.6 200,946 + GFF170 0.24 -1.1 200,947 - GFF170 0.24 -1.1
Or see this region's nucleotide sequence