Strain Fitness in Variovorax sp. SCN45 around GFF168

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF167 and GFF168 overlap by 4 nucleotidesGFF168 and GFF169 overlap by 4 nucleotidesGFF169 and GFF170 overlap by 11 nucleotides GFF167 - Two-component system sensor histidine kinase, at 195,697 to 199,197 GFF167 GFF168 - MCP methyltransferase, CheR-type, at 199,194 to 200,060 GFF168 GFF169 - Protein-glutamate methylesterase (EC 3.1.1.61), at 200,057 to 200,659 GFF169 GFF170 - Two-component system sensor histidine kinase/response regulator hybrid, at 200,649 to 201,872 GFF170 Position (kb) 199 200 201Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 198.304 kb on + strand, within GFF167at 198.304 kb on + strand, within GFF167at 198.619 kb on + strand, within GFF167at 198.619 kb on + strand, within GFF167at 198.902 kb on - strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.120 kb on + strandat 199.121 kb on - strandat 199.121 kb on - strandat 199.121 kb on - strandat 199.290 kb on + strand, within GFF168at 199.290 kb on + strand, within GFF168at 199.291 kb on - strand, within GFF168at 199.291 kb on - strand, within GFF168at 199.291 kb on - strand, within GFF168at 199.299 kb on + strand, within GFF168at 199.299 kb on + strand, within GFF168at 199.300 kb on - strand, within GFF168at 199.305 kb on + strand, within GFF168at 199.305 kb on + strand, within GFF168at 199.305 kb on + strand, within GFF168at 199.305 kb on + strand, within GFF168at 199.306 kb on - strand, within GFF168at 199.306 kb on - strand, within GFF168at 199.306 kb on - strand, within GFF168at 199.307 kb on - strand, within GFF168at 199.320 kb on + strand, within GFF168at 199.321 kb on - strand, within GFF168at 199.486 kb on - strand, within GFF168at 199.531 kb on - strand, within GFF168at 199.531 kb on - strand, within GFF168at 199.635 kb on + strand, within GFF168at 199.635 kb on + strand, within GFF168at 199.710 kb on + strand, within GFF168at 199.710 kb on + strand, within GFF168at 199.710 kb on + strand, within GFF168at 199.722 kb on + strand, within GFF168at 199.723 kb on - strand, within GFF168at 199.758 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.761 kb on + strand, within GFF168at 199.762 kb on - strand, within GFF168at 199.762 kb on - strand, within GFF168at 199.773 kb on + strand, within GFF168at 199.774 kb on - strand, within GFF168at 199.893 kb on + strand, within GFF168at 199.894 kb on - strand, within GFF168at 200.043 kb on + strandat 200.044 kb on - strandat 200.175 kb on + strand, within GFF169at 200.175 kb on + strand, within GFF169at 200.175 kb on + strand, within GFF169at 200.175 kb on + strand, within GFF169at 200.175 kb on + strand, within GFF169at 200.175 kb on + strand, within GFF169at 200.176 kb on - strand, within GFF169at 200.176 kb on - strand, within GFF169at 200.176 kb on - strand, within GFF169at 200.303 kb on + strand, within GFF169at 200.303 kb on + strand, within GFF169at 200.303 kb on + strand, within GFF169at 200.303 kb on + strand, within GFF169at 200.304 kb on - strand, within GFF169at 200.304 kb on - strand, within GFF169at 200.304 kb on - strand, within GFF169at 200.321 kb on + strand, within GFF169at 200.322 kb on - strand, within GFF169at 200.322 kb on - strand, within GFF169at 200.322 kb on - strand, within GFF169at 200.334 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.471 kb on + strand, within GFF169at 200.472 kb on - strand, within GFF169at 200.472 kb on - strand, within GFF169at 200.472 kb on - strand, within GFF169at 200.526 kb on + strand, within GFF169at 200.526 kb on + strand, within GFF169at 200.526 kb on + strand, within GFF169at 200.527 kb on - strand, within GFF169at 200.594 kb on + strand, within GFF169at 200.594 kb on + strand, within GFF169at 200.594 kb on + strand, within GFF169at 200.594 kb on + strand, within GFF169at 200.595 kb on - strand, within GFF169at 200.595 kb on - strand, within GFF169at 200.595 kb on - strand, within GFF169at 200.595 kb on - strand, within GFF169at 200.931 kb on + strand, within GFF170at 200.931 kb on + strand, within GFF170at 200.932 kb on - strand, within GFF170at 200.932 kb on - strand, within GFF170at 200.932 kb on - strand, within GFF170at 200.946 kb on + strand, within GFF170at 200.947 kb on - strand, within GFF170

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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198,304 + GFF167 0.74 -0.1
198,304 + GFF167 0.74 +0.2
198,619 + GFF167 0.83 -0.4
198,619 + GFF167 0.83 -0.1
198,902 - +0.5
199,120 + -0.0
199,120 + -1.2
199,120 + -1.8
199,120 + -1.6
199,120 + +1.2
199,120 + -1.2
199,120 + -0.6
199,121 - -0.8
199,121 - -1.6
199,121 - -0.9
199,290 + GFF168 0.11 -0.8
199,290 + GFF168 0.11 -1.1
199,291 - GFF168 0.11 -0.8
199,291 - GFF168 0.11 -1.9
199,291 - GFF168 0.11 -2.2
199,299 + GFF168 0.12 -1.6
199,299 + GFF168 0.12 +0.2
199,300 - GFF168 0.12 -3.6
199,305 + GFF168 0.13 -1.8
199,305 + GFF168 0.13 -0.6
199,305 + GFF168 0.13 +0.2
199,305 + GFF168 0.13 -0.8
199,306 - GFF168 0.13 -1.1
199,306 - GFF168 0.13 -1.8
199,306 - GFF168 0.13 -2.2
199,307 - GFF168 0.13 -2.9
199,320 + GFF168 0.15 -0.6
199,321 - GFF168 0.15 -3.7
199,486 - GFF168 0.34 -2.3
199,531 - GFF168 0.39 -1.8
199,531 - GFF168 0.39 -2.3
199,635 + GFF168 0.51 -0.6
199,635 + GFF168 0.51 +0.2
199,710 + GFF168 0.60 -1.6
199,710 + GFF168 0.60 -0.6
199,710 + GFF168 0.60 -4.3
199,722 + GFF168 0.61 -1.1
199,723 - GFF168 0.61 -3.9
199,758 + GFF168 0.65 -2.6
199,761 + GFF168 0.65 -3.1
199,761 + GFF168 0.65 -2.3
199,761 + GFF168 0.65 +0.9
199,761 + GFF168 0.65 +0.4
199,761 + GFF168 0.65 -1.8
199,761 + GFF168 0.65 -3.3
199,761 + GFF168 0.65 -0.9
199,761 + GFF168 0.65 +1.2
199,762 - GFF168 0.66 -2.1
199,762 - GFF168 0.66 -1.6
199,773 + GFF168 0.67 -1.2
199,774 - GFF168 0.67 -1.8
199,893 + GFF168 0.81 -0.8
199,894 - GFF168 0.81 -1.8
200,043 + -1.9
200,044 - -1.7
200,175 + GFF169 0.20 -1.0
200,175 + GFF169 0.20 -0.7
200,175 + GFF169 0.20 -0.9
200,175 + GFF169 0.20 -0.9
200,175 + GFF169 0.20 +0.5
200,175 + GFF169 0.20 -0.4
200,176 - GFF169 0.20 -0.7
200,176 - GFF169 0.20 -1.7
200,176 - GFF169 0.20 -1.3
200,303 + GFF169 0.41 -1.4
200,303 + GFF169 0.41 -0.6
200,303 + GFF169 0.41 -1.1
200,303 + GFF169 0.41 +0.8
200,304 - GFF169 0.41 -1.7
200,304 - GFF169 0.41 -1.3
200,304 - GFF169 0.41 -0.6
200,321 + GFF169 0.44 -0.8
200,322 - GFF169 0.44 -0.7
200,322 - GFF169 0.44 -1.0
200,322 - GFF169 0.44 -0.8
200,334 + GFF169 0.46 -0.1
200,471 + GFF169 0.69 -0.9
200,471 + GFF169 0.69 -0.7
200,471 + GFF169 0.69 -0.3
200,471 + GFF169 0.69 -1.5
200,471 + GFF169 0.69 -1.3
200,471 + GFF169 0.69 -0.9
200,471 + GFF169 0.69 -1.8
200,471 + GFF169 0.69 -1.3
200,472 - GFF169 0.69 -0.3
200,472 - GFF169 0.69 -0.1
200,472 - GFF169 0.69 +0.7
200,526 + GFF169 0.78 -0.7
200,526 + GFF169 0.78 -1.1
200,526 + GFF169 0.78 -0.6
200,527 - GFF169 0.78 -0.3
200,594 + GFF169 0.89 -0.2
200,594 + GFF169 0.89 -0.4
200,594 + GFF169 0.89 -1.9
200,594 + GFF169 0.89 -1.7
200,595 - GFF169 0.89 -1.1
200,595 - GFF169 0.89 -1.6
200,595 - GFF169 0.89 -1.0
200,595 - GFF169 0.89 -0.8
200,931 + GFF170 0.23 -0.2
200,931 + GFF170 0.23 -0.7
200,932 - GFF170 0.23 -0.3
200,932 - GFF170 0.23 -0.8
200,932 - GFF170 0.23 +3.6
200,946 + GFF170 0.24 -1.1
200,947 - GFF170 0.24 -1.1

Or see this region's nucleotide sequence