Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1574 and GFF1575 overlap by 4 nucleotides GFF1575 and GFF1576 are separated by 19 nucleotides GFF1576 and GFF1577 are separated by 19 nucleotides GFF1577 and GFF1578 are separated by 24 nucleotides
GFF1574 - Alpha-aminoadipate aminotransferase (EC 2.6.1.39) @ Leucine transaminase (EC 2.6.1.6) @ Valine transaminase, at 174,476 to 175,687
GFF1574
GFF1575 - BUG/TctC family periplasmic protein, at 175,684 to 176,655
GFF1575
GFF1576 - 2-pyrone-4,6-dicarboxylic acid hydrolase (EC 3.1.1.57), at 176,675 to 177,592
GFF1576
GFF1577 - hypothetical protein, at 177,612 to 178,052
GFF1577
GFF1578 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 178,077 to 178,706
GFF1578
Position (kb) 176
177
178 Strain fitness (log2 ratio) -2
-1
0
1 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.149 kb on + strand, within GFF1575 at 176.150 kb on - strand, within GFF1575 at 176.150 kb on - strand, within GFF1575 at 176.606 kb on - strand at 176.606 kb on - strand at 176.826 kb on - strand, within GFF1576 at 176.843 kb on + strand, within GFF1576 at 176.843 kb on + strand, within GFF1576 at 176.844 kb on - strand, within GFF1576 at 176.987 kb on + strand, within GFF1576 at 176.987 kb on + strand, within GFF1576 at 176.988 kb on - strand, within GFF1576 at 177.228 kb on - strand, within GFF1576 at 177.228 kb on - strand, within GFF1576 at 177.349 kb on + strand, within GFF1576 at 177.350 kb on - strand, within GFF1576 at 177.579 kb on - strand at 177.675 kb on + strand, within GFF1577 at 177.714 kb on + strand, within GFF1577 at 177.714 kb on + strand, within GFF1577 at 177.714 kb on + strand, within GFF1577 at 177.715 kb on - strand, within GFF1577 at 177.718 kb on - strand, within GFF1577 at 177.718 kb on - strand, within GFF1577 at 177.787 kb on - strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.882 kb on + strand, within GFF1577 at 177.883 kb on - strand, within GFF1577 at 177.898 kb on - strand, within GFF1577
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 176,149 + GFF1575 0.48 -0.3 176,149 + GFF1575 0.48 -0.8 176,149 + GFF1575 0.48 -0.2 176,149 + GFF1575 0.48 -0.1 176,150 - GFF1575 0.48 -0.2 176,150 - GFF1575 0.48 -0.9 176,606 - -0.9 176,606 - -0.6 176,826 - GFF1576 0.16 +0.4 176,843 + GFF1576 0.18 -0.7 176,843 + GFF1576 0.18 +0.8 176,844 - GFF1576 0.18 +0.0 176,987 + GFF1576 0.34 -2.2 176,987 + GFF1576 0.34 +0.4 176,988 - GFF1576 0.34 -1.5 177,228 - GFF1576 0.60 +0.6 177,228 - GFF1576 0.60 +0.5 177,349 + GFF1576 0.73 +0.8 177,350 - GFF1576 0.74 -0.1 177,579 - +1.0 177,675 + GFF1577 0.14 -0.7 177,714 + GFF1577 0.23 +0.5 177,714 + GFF1577 0.23 +0.3 177,714 + GFF1577 0.23 -0.3 177,715 - GFF1577 0.23 +0.3 177,718 - GFF1577 0.24 +0.7 177,718 - GFF1577 0.24 +0.7 177,787 - GFF1577 0.40 +0.2 177,882 + GFF1577 0.61 -1.2 177,882 + GFF1577 0.61 +0.1 177,882 + GFF1577 0.61 -1.5 177,883 - GFF1577 0.61 -0.3 177,898 - GFF1577 0.65 +0.3
Or see this region's nucleotide sequence