Strain Fitness in Variovorax sp. SCN45 around GFF1519

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1517 and GFF1518 are separated by 47 nucleotidesGFF1518 and GFF1519 overlap by 4 nucleotidesGFF1519 and GFF1520 are separated by 158 nucleotidesGFF1520 and GFF1521 are separated by 25 nucleotides GFF1517 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 112,820 to 113,383 GFF1517 GFF1518 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 113,431 to 113,901 GFF1518 GFF1519 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 113,898 to 114,899 GFF1519 GFF1520 - Transcriptional regulator, IclR family, at 115,058 to 115,840 GFF1520 GFF1521 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 115,866 to 116,885 GFF1521 Position (kb) 113 114 115Strain fitness (log2 ratio) -3 -2 -1 0 1at 112.998 kb on + strand, within GFF1517at 112.999 kb on - strand, within GFF1517at 113.094 kb on + strand, within GFF1517at 113.094 kb on + strand, within GFF1517at 113.094 kb on + strand, within GFF1517at 113.280 kb on + strand, within GFF1517at 113.486 kb on + strand, within GFF1518at 113.487 kb on - strand, within GFF1518at 113.814 kb on - strand, within GFF1518at 113.814 kb on - strand, within GFF1518at 113.817 kb on + strand, within GFF1518at 113.817 kb on + strand, within GFF1518at 113.817 kb on + strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.818 kb on - strand, within GFF1518at 113.956 kb on + strandat 114.103 kb on + strand, within GFF1519at 114.103 kb on + strand, within GFF1519at 114.202 kb on + strand, within GFF1519at 114.203 kb on - strand, within GFF1519at 114.203 kb on - strand, within GFF1519at 114.203 kb on - strand, within GFF1519at 114.313 kb on + strand, within GFF1519at 114.313 kb on + strand, within GFF1519at 114.313 kb on + strand, within GFF1519at 114.476 kb on - strand, within GFF1519at 114.709 kb on + strand, within GFF1519at 114.709 kb on + strand, within GFF1519at 114.710 kb on - strand, within GFF1519at 114.884 kb on + strandat 114.884 kb on + strandat 114.885 kb on - strandat 114.997 kb on - strandat 115.004 kb on + strandat 115.005 kb on - strandat 115.005 kb on - strandat 115.097 kb on + strandat 115.205 kb on + strand, within GFF1520at 115.235 kb on + strand, within GFF1520at 115.235 kb on + strand, within GFF1520at 115.235 kb on + strand, within GFF1520at 115.236 kb on - strand, within GFF1520at 115.236 kb on - strand, within GFF1520at 115.425 kb on - strand, within GFF1520at 115.425 kb on - strand, within GFF1520at 115.554 kb on - strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.595 kb on + strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.596 kb on - strand, within GFF1520at 115.822 kb on - strandat 115.822 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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112,998 + GFF1517 0.32 +0.3
112,999 - GFF1517 0.32 -0.1
113,094 + GFF1517 0.49 -0.3
113,094 + GFF1517 0.49 +0.6
113,094 + GFF1517 0.49 +0.1
113,280 + GFF1517 0.82 +1.1
113,486 + GFF1518 0.12 +0.1
113,487 - GFF1518 0.12 -2.8
113,814 - GFF1518 0.81 -0.1
113,814 - GFF1518 0.81 -0.4
113,817 + GFF1518 0.82 -0.4
113,817 + GFF1518 0.82 +0.3
113,817 + GFF1518 0.82 -0.9
113,818 - GFF1518 0.82 +0.6
113,818 - GFF1518 0.82 -0.5
113,818 - GFF1518 0.82 -0.4
113,818 - GFF1518 0.82 -0.4
113,818 - GFF1518 0.82 +0.2
113,818 - GFF1518 0.82 -1.2
113,818 - GFF1518 0.82 -1.5
113,956 + +0.2
114,103 + GFF1519 0.20 -0.4
114,103 + GFF1519 0.20 -0.3
114,202 + GFF1519 0.30 -0.1
114,203 - GFF1519 0.30 +0.6
114,203 - GFF1519 0.30 -0.2
114,203 - GFF1519 0.30 -0.9
114,313 + GFF1519 0.41 -0.2
114,313 + GFF1519 0.41 -1.3
114,313 + GFF1519 0.41 -0.2
114,476 - GFF1519 0.58 +0.9
114,709 + GFF1519 0.81 +0.3
114,709 + GFF1519 0.81 -0.4
114,710 - GFF1519 0.81 +0.1
114,884 + +1.0
114,884 + -0.4
114,885 - -0.2
114,997 - -0.1
115,004 + +0.3
115,005 - -0.6
115,005 - -0.6
115,097 + -0.7
115,205 + GFF1520 0.19 +0.5
115,235 + GFF1520 0.23 -1.9
115,235 + GFF1520 0.23 -0.2
115,235 + GFF1520 0.23 -1.4
115,236 - GFF1520 0.23 -0.4
115,236 - GFF1520 0.23 +1.6
115,425 - GFF1520 0.47 -0.9
115,425 - GFF1520 0.47 +0.9
115,554 - GFF1520 0.63 -0.5
115,595 + GFF1520 0.69 -0.5
115,595 + GFF1520 0.69 -1.4
115,595 + GFF1520 0.69 -0.6
115,595 + GFF1520 0.69 +0.1
115,595 + GFF1520 0.69 -0.9
115,595 + GFF1520 0.69 -1.3
115,595 + GFF1520 0.69 -0.8
115,595 + GFF1520 0.69 -0.8
115,595 + GFF1520 0.69 -1.5
115,596 - GFF1520 0.69 -0.4
115,596 - GFF1520 0.69 -0.5
115,596 - GFF1520 0.69 +0.3
115,596 - GFF1520 0.69 -0.4
115,596 - GFF1520 0.69 -0.4
115,596 - GFF1520 0.69 -0.2
115,822 - -0.7
115,822 - -1.5

Or see this region's nucleotide sequence