Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1517 and GFF1518 are separated by 47 nucleotides GFF1518 and GFF1519 overlap by 4 nucleotides GFF1519 and GFF1520 are separated by 158 nucleotides GFF1520 and GFF1521 are separated by 25 nucleotides
GFF1517 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 112,820 to 113,383
GFF1517
GFF1518 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 113,431 to 113,901
GFF1518
GFF1519 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 113,898 to 114,899
GFF1519
GFF1520 - Transcriptional regulator, IclR family, at 115,058 to 115,840
GFF1520
GFF1521 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 115,866 to 116,885
GFF1521
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 112.998 kb on + strand, within GFF1517 at 112.999 kb on - strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.280 kb on + strand, within GFF1517 at 113.486 kb on + strand, within GFF1518 at 113.487 kb on - strand, within GFF1518 at 113.814 kb on - strand, within GFF1518 at 113.814 kb on - strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.956 kb on + strand at 114.103 kb on + strand, within GFF1519 at 114.103 kb on + strand, within GFF1519 at 114.202 kb on + strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.476 kb on - strand, within GFF1519 at 114.709 kb on + strand, within GFF1519 at 114.709 kb on + strand, within GFF1519 at 114.710 kb on - strand, within GFF1519 at 114.884 kb on + strand at 114.884 kb on + strand at 114.885 kb on - strand at 114.997 kb on - strand at 115.004 kb on + strand at 115.005 kb on - strand at 115.005 kb on - strand at 115.097 kb on + strand at 115.205 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.236 kb on - strand, within GFF1520 at 115.236 kb on - strand, within GFF1520 at 115.425 kb on - strand, within GFF1520 at 115.425 kb on - strand, within GFF1520 at 115.554 kb on - strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.822 kb on - strand at 115.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 112,998 + GFF1517 0.32 +0.3 112,999 - GFF1517 0.32 -0.1 113,094 + GFF1517 0.49 -0.3 113,094 + GFF1517 0.49 +0.6 113,094 + GFF1517 0.49 +0.1 113,280 + GFF1517 0.82 +1.1 113,486 + GFF1518 0.12 +0.1 113,487 - GFF1518 0.12 -2.8 113,814 - GFF1518 0.81 -0.1 113,814 - GFF1518 0.81 -0.4 113,817 + GFF1518 0.82 -0.4 113,817 + GFF1518 0.82 +0.3 113,817 + GFF1518 0.82 -0.9 113,818 - GFF1518 0.82 +0.6 113,818 - GFF1518 0.82 -0.5 113,818 - GFF1518 0.82 -0.4 113,818 - GFF1518 0.82 -0.4 113,818 - GFF1518 0.82 +0.2 113,818 - GFF1518 0.82 -1.2 113,818 - GFF1518 0.82 -1.5 113,956 + +0.2 114,103 + GFF1519 0.20 -0.4 114,103 + GFF1519 0.20 -0.3 114,202 + GFF1519 0.30 -0.1 114,203 - GFF1519 0.30 +0.6 114,203 - GFF1519 0.30 -0.2 114,203 - GFF1519 0.30 -0.9 114,313 + GFF1519 0.41 -0.2 114,313 + GFF1519 0.41 -1.3 114,313 + GFF1519 0.41 -0.2 114,476 - GFF1519 0.58 +0.9 114,709 + GFF1519 0.81 +0.3 114,709 + GFF1519 0.81 -0.4 114,710 - GFF1519 0.81 +0.1 114,884 + +1.0 114,884 + -0.4 114,885 - -0.2 114,997 - -0.1 115,004 + +0.3 115,005 - -0.6 115,005 - -0.6 115,097 + -0.7 115,205 + GFF1520 0.19 +0.5 115,235 + GFF1520 0.23 -1.9 115,235 + GFF1520 0.23 -0.2 115,235 + GFF1520 0.23 -1.4 115,236 - GFF1520 0.23 -0.4 115,236 - GFF1520 0.23 +1.6 115,425 - GFF1520 0.47 -0.9 115,425 - GFF1520 0.47 +0.9 115,554 - GFF1520 0.63 -0.5 115,595 + GFF1520 0.69 -0.5 115,595 + GFF1520 0.69 -1.4 115,595 + GFF1520 0.69 -0.6 115,595 + GFF1520 0.69 +0.1 115,595 + GFF1520 0.69 -0.9 115,595 + GFF1520 0.69 -1.3 115,595 + GFF1520 0.69 -0.8 115,595 + GFF1520 0.69 -0.8 115,595 + GFF1520 0.69 -1.5 115,596 - GFF1520 0.69 -0.4 115,596 - GFF1520 0.69 -0.5 115,596 - GFF1520 0.69 +0.3 115,596 - GFF1520 0.69 -0.4 115,596 - GFF1520 0.69 -0.4 115,596 - GFF1520 0.69 -0.2 115,822 - -0.7 115,822 - -1.5
Or see this region's nucleotide sequence