Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF12 and GFF13 are separated by 35 nucleotides GFF13 and GFF14 overlap by 32 nucleotides GFF14 and GFF15 are separated by 152 nucleotides GFF15 and GFF16 are separated by 3 nucleotides
GFF12 - Two-component system sensor histidine kinase, at 12,734 to 14,143
GFF12
GFF13 - BUG/TctC family periplasmic protein, at 14,179 to 14,289
GFF13
GFF14 - N-acetyltransferase ElaA, at 14,258 to 14,656
GFF14
GFF15 - no description, at 14,809 to 15,117
GFF15
GFF16 - Cellulose synthase (UDP-forming) (EC 2.4.1.12) / Efflux transport system, outer membrane factor (OMF) lipoprotein, at 15,121 to 18,555
GFF16
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1 at 13.983 kb on - strand, within GFF12 at 14.287 kb on + strand at 14.334 kb on + strand, within GFF14 at 14.335 kb on - strand, within GFF14 at 14.370 kb on + strand, within GFF14 at 14.370 kb on + strand, within GFF14 at 14.371 kb on - strand, within GFF14 at 14.371 kb on - strand, within GFF14 at 14.557 kb on - strand, within GFF14 at 14.586 kb on + strand, within GFF14 at 14.628 kb on + strand at 14.629 kb on - strand at 14.629 kb on - strand at 14.682 kb on + strand at 14.682 kb on + strand at 14.683 kb on - strand at 14.683 kb on - strand at 14.683 kb on - strand at 14.716 kb on - strand at 14.755 kb on + strand at 14.755 kb on + strand at 14.756 kb on - strand at 14.756 kb on - strand at 14.774 kb on + strand at 14.775 kb on - strand at 14.836 kb on + strand at 14.836 kb on + strand at 14.837 kb on - strand at 14.837 kb on - strand at 14.887 kb on + strand, within GFF15 at 14.902 kb on + strand, within GFF15 at 15.188 kb on + strand at 15.188 kb on + strand at 15.189 kb on - strand at 15.189 kb on - strand at 15.251 kb on + strand at 15.251 kb on + strand at 15.251 kb on + strand at 15.252 kb on - strand at 15.252 kb on - strand at 15.252 kb on - strand at 15.252 kb on - strand at 15.252 kb on - strand at 15.437 kb on + strand at 15.437 kb on + strand at 15.444 kb on - strand at 15.626 kb on + strand, within GFF16 at 15.626 kb on + strand, within GFF16
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 13,983 - GFF12 0.89 -1.4 14,287 + -0.8 14,334 + GFF14 0.19 +0.7 14,335 - GFF14 0.19 +0.2 14,370 + GFF14 0.28 +1.7 14,370 + GFF14 0.28 +0.2 14,371 - GFF14 0.28 -0.4 14,371 - GFF14 0.28 -1.6 14,557 - GFF14 0.75 -0.7 14,586 + GFF14 0.82 -0.6 14,628 + -0.8 14,629 - -1.3 14,629 - +0.6 14,682 + +0.4 14,682 + -0.1 14,683 - -0.0 14,683 - -0.2 14,683 - -0.9 14,716 - -1.1 14,755 + -0.9 14,755 + -0.1 14,756 - -0.6 14,756 - -0.8 14,774 + -0.8 14,775 - +0.6 14,836 + -0.9 14,836 + +0.0 14,837 - -0.8 14,837 - -0.2 14,887 + GFF15 0.25 -0.6 14,902 + GFF15 0.30 -1.2 15,188 + -0.4 15,188 + +1.2 15,189 - -0.9 15,189 - -1.8 15,251 + -1.8 15,251 + -0.3 15,251 + -2.1 15,252 - -0.1 15,252 - +0.6 15,252 - -0.5 15,252 - -0.6 15,252 - +0.4 15,437 + -0.8 15,437 + +0.0 15,444 - +0.1 15,626 + GFF16 0.15 -0.4 15,626 + GFF16 0.15 -0.8
Or see this region's nucleotide sequence