Strain Fitness in Variovorax sp. SCN45 around GFF15

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF12 and GFF13 are separated by 35 nucleotidesGFF13 and GFF14 overlap by 32 nucleotidesGFF14 and GFF15 are separated by 152 nucleotidesGFF15 and GFF16 are separated by 3 nucleotides GFF12 - Two-component system sensor histidine kinase, at 12,734 to 14,143 GFF12 GFF13 - BUG/TctC family periplasmic protein, at 14,179 to 14,289 GFF13 GFF14 - N-acetyltransferase ElaA, at 14,258 to 14,656 GFF14 GFF15 - no description, at 14,809 to 15,117 GFF15 GFF16 - Cellulose synthase (UDP-forming) (EC 2.4.1.12) / Efflux transport system, outer membrane factor (OMF) lipoprotein, at 15,121 to 18,555 GFF16 Position (kb) 14 15 16Strain fitness (log2 ratio) -2 -1 0 1at 13.983 kb on - strand, within GFF12at 14.287 kb on + strandat 14.334 kb on + strand, within GFF14at 14.335 kb on - strand, within GFF14at 14.370 kb on + strand, within GFF14at 14.370 kb on + strand, within GFF14at 14.371 kb on - strand, within GFF14at 14.371 kb on - strand, within GFF14at 14.557 kb on - strand, within GFF14at 14.586 kb on + strand, within GFF14at 14.628 kb on + strandat 14.629 kb on - strandat 14.629 kb on - strandat 14.682 kb on + strandat 14.682 kb on + strandat 14.683 kb on - strandat 14.683 kb on - strandat 14.683 kb on - strandat 14.716 kb on - strandat 14.755 kb on + strandat 14.755 kb on + strandat 14.756 kb on - strandat 14.756 kb on - strandat 14.774 kb on + strandat 14.775 kb on - strandat 14.836 kb on + strandat 14.836 kb on + strandat 14.837 kb on - strandat 14.837 kb on - strandat 14.887 kb on + strand, within GFF15at 14.902 kb on + strand, within GFF15at 15.188 kb on + strandat 15.188 kb on + strandat 15.189 kb on - strandat 15.189 kb on - strandat 15.251 kb on + strandat 15.251 kb on + strandat 15.251 kb on + strandat 15.252 kb on - strandat 15.252 kb on - strandat 15.252 kb on - strandat 15.252 kb on - strandat 15.252 kb on - strandat 15.437 kb on + strandat 15.437 kb on + strandat 15.444 kb on - strandat 15.626 kb on + strand, within GFF16at 15.626 kb on + strand, within GFF16

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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13,983 - GFF12 0.89 -1.4
14,287 + -0.8
14,334 + GFF14 0.19 +0.7
14,335 - GFF14 0.19 +0.2
14,370 + GFF14 0.28 +1.7
14,370 + GFF14 0.28 +0.2
14,371 - GFF14 0.28 -0.4
14,371 - GFF14 0.28 -1.6
14,557 - GFF14 0.75 -0.7
14,586 + GFF14 0.82 -0.6
14,628 + -0.8
14,629 - -1.3
14,629 - +0.6
14,682 + +0.4
14,682 + -0.1
14,683 - -0.0
14,683 - -0.2
14,683 - -0.9
14,716 - -1.1
14,755 + -0.9
14,755 + -0.1
14,756 - -0.6
14,756 - -0.8
14,774 + -0.8
14,775 - +0.6
14,836 + -0.9
14,836 + +0.0
14,837 - -0.8
14,837 - -0.2
14,887 + GFF15 0.25 -0.6
14,902 + GFF15 0.30 -1.2
15,188 + -0.4
15,188 + +1.2
15,189 - -0.9
15,189 - -1.8
15,251 + -1.8
15,251 + -0.3
15,251 + -2.1
15,252 - -0.1
15,252 - +0.6
15,252 - -0.5
15,252 - -0.6
15,252 - +0.4
15,437 + -0.8
15,437 + +0.0
15,444 - +0.1
15,626 + GFF16 0.15 -0.4
15,626 + GFF16 0.15 -0.8

Or see this region's nucleotide sequence