Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1497 and GFF1498 overlap by 4 nucleotides GFF1498 and GFF1499 are separated by 6 nucleotides GFF1499 and GFF1500 are separated by 130 nucleotides
GFF1497 - BUG/TctC family periplasmic protein, at 89,847 to 90,833
GFF1497
GFF1498 - putative 2-pyrone-4,6-dicarboxylic acid hydrolase, at 90,830 to 91,750
GFF1498
GFF1499 - BUG/TctC family periplasmic protein, at 91,757 to 92,743
GFF1499
GFF1500 - Transcriptional regulator, IclR family, at 92,874 to 93,683
GFF1500
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 90.870 kb on + strand at 90.870 kb on + strand at 90.871 kb on - strand at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.104 kb on + strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.105 kb on - strand, within GFF1498 at 91.214 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498 at 91.509 kb on + strand, within GFF1498 at 92.001 kb on + strand, within GFF1499 at 92.001 kb on + strand, within GFF1499 at 92.001 kb on + strand, within GFF1499 at 92.002 kb on - strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.124 kb on + strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.125 kb on - strand, within GFF1499 at 92.178 kb on + strand, within GFF1499 at 92.178 kb on + strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.179 kb on - strand, within GFF1499 at 92.265 kb on + strand, within GFF1499 at 92.404 kb on - strand, within GFF1499 at 92.419 kb on - strand, within GFF1499 at 92.469 kb on + strand, within GFF1499 at 92.470 kb on - strand, within GFF1499 at 92.653 kb on - strand at 92.653 kb on - strand at 92.737 kb on + strand at 92.737 kb on + strand at 92.737 kb on + strand at 92.738 kb on - strand at 92.826 kb on + strand at 92.826 kb on + strand at 92.826 kb on + strand at 92.826 kb on + strand at 92.827 kb on - strand at 92.827 kb on - strand at 93.117 kb on + strand, within GFF1500 at 93.117 kb on + strand, within GFF1500 at 93.118 kb on - strand, within GFF1500 at 93.118 kb on - strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.267 kb on + strand, within GFF1500 at 93.268 kb on - strand, within GFF1500 at 93.268 kb on - strand, within GFF1500 at 93.268 kb on - strand, within GFF1500 at 93.420 kb on + strand, within GFF1500 at 93.420 kb on + strand, within GFF1500 at 93.420 kb on + strand, within GFF1500 at 93.421 kb on - strand, within GFF1500 at 93.421 kb on - strand, within GFF1500 at 93.444 kb on + strand, within GFF1500 at 93.444 kb on + strand, within GFF1500 at 93.445 kb on - strand, within GFF1500 at 93.445 kb on - strand, within GFF1500 at 93.445 kb on - strand, within GFF1500 at 93.445 kb on - strand, within GFF1500 at 93.445 kb on - strand, within GFF1500 at 93.621 kb on + strand at 93.622 kb on - strand at 93.681 kb on + strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.682 kb on - strand at 93.727 kb on - strand at 93.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 90,870 + -0.5 90,870 + +2.6 90,871 - -0.3 91,104 + GFF1498 0.30 +0.3 91,104 + GFF1498 0.30 -0.1 91,104 + GFF1498 0.30 +0.7 91,104 + GFF1498 0.30 +0.2 91,105 - GFF1498 0.30 -0.8 91,105 - GFF1498 0.30 -0.0 91,105 - GFF1498 0.30 -1.2 91,214 + GFF1498 0.42 +0.6 91,509 + GFF1498 0.74 +1.1 91,509 + GFF1498 0.74 -0.4 92,001 + GFF1499 0.25 -0.3 92,001 + GFF1499 0.25 -0.0 92,001 + GFF1499 0.25 -0.6 92,002 - GFF1499 0.25 -0.1 92,124 + GFF1499 0.37 -0.4 92,124 + GFF1499 0.37 -0.0 92,124 + GFF1499 0.37 -0.9 92,124 + GFF1499 0.37 -0.6 92,124 + GFF1499 0.37 -0.9 92,124 + GFF1499 0.37 +0.1 92,124 + GFF1499 0.37 +0.6 92,124 + GFF1499 0.37 +0.6 92,124 + GFF1499 0.37 -0.0 92,124 + GFF1499 0.37 -0.2 92,124 + GFF1499 0.37 +0.8 92,124 + GFF1499 0.37 +1.3 92,124 + GFF1499 0.37 -0.1 92,124 + GFF1499 0.37 -0.3 92,124 + GFF1499 0.37 +0.3 92,125 - GFF1499 0.37 +0.1 92,125 - GFF1499 0.37 +0.6 92,125 - GFF1499 0.37 -0.1 92,125 - GFF1499 0.37 +0.2 92,125 - GFF1499 0.37 +1.2 92,125 - GFF1499 0.37 -0.8 92,125 - GFF1499 0.37 -0.4 92,125 - GFF1499 0.37 -0.2 92,178 + GFF1499 0.43 -0.7 92,178 + GFF1499 0.43 -0.6 92,179 - GFF1499 0.43 -0.6 92,179 - GFF1499 0.43 +0.8 92,179 - GFF1499 0.43 -0.1 92,265 + GFF1499 0.51 +1.1 92,404 - GFF1499 0.66 +1.3 92,419 - GFF1499 0.67 -0.5 92,469 + GFF1499 0.72 +0.1 92,470 - GFF1499 0.72 -0.8 92,653 - +0.9 92,653 - -0.5 92,737 + +0.1 92,737 + -1.1 92,737 + -0.2 92,738 - +1.1 92,826 + +0.1 92,826 + +0.1 92,826 + +0.1 92,826 + -0.4 92,827 - -0.5 92,827 - +0.1 93,117 + GFF1500 0.30 -0.1 93,117 + GFF1500 0.30 +0.4 93,118 - GFF1500 0.30 +0.8 93,118 - GFF1500 0.30 +0.2 93,267 + GFF1500 0.49 -0.1 93,267 + GFF1500 0.49 +0.6 93,267 + GFF1500 0.49 -1.2 93,267 + GFF1500 0.49 -0.2 93,267 + GFF1500 0.49 +2.2 93,267 + GFF1500 0.49 -0.3 93,268 - GFF1500 0.49 -0.1 93,268 - GFF1500 0.49 +0.3 93,268 - GFF1500 0.49 -0.2 93,420 + GFF1500 0.67 +1.1 93,420 + GFF1500 0.67 -0.8 93,420 + GFF1500 0.67 -0.1 93,421 - GFF1500 0.68 -0.6 93,421 - GFF1500 0.68 -0.7 93,444 + GFF1500 0.70 -0.2 93,444 + GFF1500 0.70 -0.1 93,445 - GFF1500 0.70 -0.9 93,445 - GFF1500 0.70 -0.9 93,445 - GFF1500 0.70 -1.5 93,445 - GFF1500 0.70 +1.6 93,445 - GFF1500 0.70 +0.5 93,621 + +0.6 93,622 - -0.7 93,681 + -1.2 93,682 - +0.6 93,682 - -0.2 93,682 - +0.9 93,682 - -0.2 93,682 - +0.1 93,682 - -0.6 93,727 - -2.1 93,727 - -0.6
Or see this region's nucleotide sequence