Strain Fitness in Variovorax sp. SCN45 around GFF1412

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1412 and GFF1413 are separated by 5 nucleotides GFF1412 - Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-), at 297 to 2,501 GFF1412 GFF1413 - hypothetical protein, at 2,507 to 4,216 GFF1413 Position (kb) 0 1 2 3Strain fitness (log2 ratio) -2 -1 0 1 2at 0.432 kb on + strandat 0.432 kb on + strandat 0.433 kb on - strandat 0.739 kb on - strand, within GFF1412at 0.795 kb on + strand, within GFF1412at 0.976 kb on + strand, within GFF1412at 0.977 kb on - strand, within GFF1412at 1.144 kb on + strand, within GFF1412at 1.145 kb on - strand, within GFF1412at 1.860 kb on - strand, within GFF1412at 1.870 kb on + strand, within GFF1412at 1.991 kb on - strand, within GFF1412at 2.366 kb on + strandat 2.650 kb on + strandat 2.651 kb on - strandat 2.651 kb on - strandat 2.654 kb on + strandat 2.655 kb on - strandat 2.655 kb on - strandat 2.668 kb on + strandat 2.669 kb on - strandat 2.669 kb on - strandat 2.877 kb on - strand, within GFF1413at 2.993 kb on + strand, within GFF1413at 3.134 kb on + strand, within GFF1413at 3.273 kb on - strand, within GFF1413

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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432 + +0.2
432 + -0.7
433 - -0.2
739 - GFF1412 0.20 -2.2
795 + GFF1412 0.23 +0.5
976 + GFF1412 0.31 +0.0
977 - GFF1412 0.31 +0.5
1,144 + GFF1412 0.38 +0.4
1,145 - GFF1412 0.38 +2.0
1,860 - GFF1412 0.71 +0.2
1,870 + GFF1412 0.71 -1.2
1,991 - GFF1412 0.77 -0.3
2,366 + +0.1
2,650 + +0.4
2,651 - +1.4
2,651 - -0.1
2,654 + -0.7
2,655 - -0.7
2,655 - -0.3
2,668 + -0.9
2,669 - +2.0
2,669 - -0.4
2,877 - GFF1413 0.22 -0.6
2,993 + GFF1413 0.28 +0.7
3,134 + GFF1413 0.37 +0.4
3,273 - GFF1413 0.45 -0.1

Or see this region's nucleotide sequence