Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1371 and GFF1372 are separated by 40 nucleotides GFF1372 and GFF1373 are separated by 2 nucleotides GFF1373 and GFF1374 are separated by 9 nucleotides
GFF1371 - Fe-S protein, homolog of lactate dehydrogenase SO1521, at 715,405 to 718,482
GFF1371
GFF1372 - Uncharacterized MFS-type transporter, at 718,523 to 719,773
GFF1372
GFF1373 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 719,776 to 720,312
GFF1373
GFF1374 - hypothetical protein, at 720,322 to 721,572
GFF1374
Position (kb) 718
719
720 Strain fitness (log2 ratio) -2
-1
0
1
2 at 717.820 kb on + strand, within GFF1371 at 717.821 kb on - strand, within GFF1371 at 717.931 kb on - strand, within GFF1371 at 718.391 kb on + strand at 718.391 kb on + strand at 718.392 kb on - strand at 718.392 kb on - strand at 718.392 kb on - strand at 718.431 kb on - strand at 718.700 kb on + strand, within GFF1372 at 718.700 kb on + strand, within GFF1372 at 718.701 kb on - strand, within GFF1372 at 718.701 kb on - strand, within GFF1372 at 718.701 kb on - strand, within GFF1372 at 718.743 kb on - strand, within GFF1372 at 718.991 kb on + strand, within GFF1372 at 718.991 kb on + strand, within GFF1372 at 718.991 kb on + strand, within GFF1372 at 718.992 kb on - strand, within GFF1372 at 718.992 kb on - strand, within GFF1372 at 718.992 kb on - strand, within GFF1372 at 718.992 kb on - strand, within GFF1372 at 718.992 kb on - strand, within GFF1372 at 719.200 kb on + strand, within GFF1372 at 719.200 kb on + strand, within GFF1372 at 719.200 kb on + strand, within GFF1372 at 719.200 kb on + strand, within GFF1372 at 719.253 kb on - strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.273 kb on + strand, within GFF1372 at 719.274 kb on - strand, within GFF1372 at 719.282 kb on + strand, within GFF1372 at 719.282 kb on + strand, within GFF1372 at 719.282 kb on + strand, within GFF1372 at 719.283 kb on - strand, within GFF1372 at 719.283 kb on - strand, within GFF1372 at 719.283 kb on - strand, within GFF1372 at 719.283 kb on - strand, within GFF1372 at 719.348 kb on + strand, within GFF1372 at 719.348 kb on + strand, within GFF1372 at 719.348 kb on + strand, within GFF1372 at 719.348 kb on + strand, within GFF1372 at 720.052 kb on + strand, within GFF1373 at 720.052 kb on + strand, within GFF1373 at 720.052 kb on + strand, within GFF1373 at 720.052 kb on + strand, within GFF1373 at 720.053 kb on - strand, within GFF1373 at 720.094 kb on + strand, within GFF1373 at 720.094 kb on + strand, within GFF1373 at 720.311 kb on - strand at 720.514 kb on + strand, within GFF1374 at 720.767 kb on + strand, within GFF1374
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 717,820 + GFF1371 0.78 +0.1 717,821 - GFF1371 0.78 -0.2 717,931 - GFF1371 0.82 +0.9 718,391 + +1.0 718,391 + +1.6 718,392 - +0.3 718,392 - +1.1 718,392 - +0.6 718,431 - -0.3 718,700 + GFF1372 0.14 +0.8 718,700 + GFF1372 0.14 +0.6 718,701 - GFF1372 0.14 -1.5 718,701 - GFF1372 0.14 +0.6 718,701 - GFF1372 0.14 +0.5 718,743 - GFF1372 0.18 -0.6 718,991 + GFF1372 0.37 -0.4 718,991 + GFF1372 0.37 +0.2 718,991 + GFF1372 0.37 -2.0 718,992 - GFF1372 0.37 +0.2 718,992 - GFF1372 0.37 +1.8 718,992 - GFF1372 0.37 +0.2 718,992 - GFF1372 0.37 +1.6 718,992 - GFF1372 0.37 -0.1 719,200 + GFF1372 0.54 +0.1 719,200 + GFF1372 0.54 -1.3 719,200 + GFF1372 0.54 -1.5 719,200 + GFF1372 0.54 +0.9 719,253 - GFF1372 0.58 -0.6 719,273 + GFF1372 0.60 +0.4 719,273 + GFF1372 0.60 -1.8 719,273 + GFF1372 0.60 -0.0 719,273 + GFF1372 0.60 -0.7 719,273 + GFF1372 0.60 -0.4 719,273 + GFF1372 0.60 +1.1 719,274 - GFF1372 0.60 -0.8 719,282 + GFF1372 0.61 -0.3 719,282 + GFF1372 0.61 +0.6 719,282 + GFF1372 0.61 -0.7 719,283 - GFF1372 0.61 +1.6 719,283 - GFF1372 0.61 -0.1 719,283 - GFF1372 0.61 +1.1 719,283 - GFF1372 0.61 -0.4 719,348 + GFF1372 0.66 -1.0 719,348 + GFF1372 0.66 +0.2 719,348 + GFF1372 0.66 -0.2 719,348 + GFF1372 0.66 -1.0 720,052 + GFF1373 0.51 -0.6 720,052 + GFF1373 0.51 -0.6 720,052 + GFF1373 0.51 -1.0 720,052 + GFF1373 0.51 -0.7 720,053 - GFF1373 0.52 -1.0 720,094 + GFF1373 0.59 +0.1 720,094 + GFF1373 0.59 -0.9 720,311 - +0.6 720,514 + GFF1374 0.15 -0.4 720,767 + GFF1374 0.36 -0.4
Or see this region's nucleotide sequence