Strain Fitness in Variovorax sp. SCN45 around GFF1350

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1348 and GFF1349 are separated by 9 nucleotidesGFF1349 and GFF1350 are separated by 24 nucleotidesGFF1350 and GFF1351 are separated by 98 nucleotides GFF1348 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 691,275 to 692,072 GFF1348 GFF1349 - Thioesterase, at 692,082 to 692,546 GFF1349 GFF1350 - Quinone oxidoreductase (EC 1.6.5.5), at 692,571 to 693,587 GFF1350 GFF1351 - Acyl-CoA dehydrogenase, at 693,686 to 695,326 GFF1351 Position (kb) 692 693 694Strain fitness (log2 ratio) -2 -1 0 1 2at 691.650 kb on + strand, within GFF1348at 691.684 kb on - strand, within GFF1348at 691.773 kb on + strand, within GFF1348at 691.774 kb on - strand, within GFF1348at 691.774 kb on - strand, within GFF1348at 691.836 kb on + strand, within GFF1348at 691.836 kb on + strand, within GFF1348at 691.836 kb on + strand, within GFF1348at 691.837 kb on - strand, within GFF1348at 691.837 kb on - strand, within GFF1348at 691.837 kb on - strand, within GFF1348at 691.837 kb on - strand, within GFF1348at 692.199 kb on + strand, within GFF1349at 692.200 kb on - strand, within GFF1349at 692.200 kb on - strand, within GFF1349at 692.431 kb on - strand, within GFF1349at 692.431 kb on - strand, within GFF1349at 692.478 kb on + strand, within GFF1349at 692.566 kb on + strandat 692.567 kb on - strandat 692.567 kb on - strandat 692.697 kb on + strand, within GFF1350at 692.697 kb on + strand, within GFF1350at 692.697 kb on + strand, within GFF1350at 692.697 kb on + strand, within GFF1350at 692.697 kb on + strand, within GFF1350at 692.697 kb on + strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.698 kb on - strand, within GFF1350at 692.715 kb on + strand, within GFF1350at 692.715 kb on + strand, within GFF1350at 692.715 kb on + strand, within GFF1350at 692.716 kb on - strand, within GFF1350at 692.716 kb on - strand, within GFF1350at 692.748 kb on + strand, within GFF1350at 692.748 kb on + strand, within GFF1350at 692.749 kb on - strand, within GFF1350at 692.826 kb on + strand, within GFF1350at 692.826 kb on + strand, within GFF1350at 692.826 kb on + strand, within GFF1350at 692.827 kb on - strand, within GFF1350at 692.827 kb on - strand, within GFF1350at 692.827 kb on - strand, within GFF1350at 692.827 kb on - strand, within GFF1350at 692.827 kb on - strand, within GFF1350at 692.871 kb on + strand, within GFF1350at 692.871 kb on + strand, within GFF1350at 692.872 kb on - strand, within GFF1350at 692.872 kb on - strand, within GFF1350at 692.983 kb on - strand, within GFF1350at 693.132 kb on + strand, within GFF1350at 693.132 kb on + strand, within GFF1350at 693.133 kb on - strand, within GFF1350at 693.133 kb on - strand, within GFF1350at 693.133 kb on - strand, within GFF1350at 693.133 kb on - strand, within GFF1350at 693.264 kb on + strand, within GFF1350at 693.264 kb on + strand, within GFF1350at 693.265 kb on - strand, within GFF1350at 693.265 kb on - strand, within GFF1350at 693.265 kb on - strand, within GFF1350at 693.509 kb on + strandat 693.679 kb on + strandat 693.679 kb on + strandat 693.734 kb on + strandat 693.734 kb on + strandat 693.734 kb on + strandat 693.734 kb on + strandat 693.734 kb on + strandat 693.735 kb on - strandat 693.735 kb on - strandat 693.944 kb on + strand, within GFF1351at 693.944 kb on + strand, within GFF1351at 693.945 kb on - strand, within GFF1351at 693.945 kb on - strand, within GFF1351at 694.094 kb on + strand, within GFF1351at 694.094 kb on + strand, within GFF1351at 694.095 kb on - strand, within GFF1351at 694.095 kb on - strand, within GFF1351at 694.134 kb on - strand, within GFF1351at 694.134 kb on - strand, within GFF1351at 694.310 kb on + strand, within GFF1351

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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691,650 + GFF1348 0.47 -0.8
691,684 - GFF1348 0.51 +0.3
691,773 + GFF1348 0.62 +0.3
691,774 - GFF1348 0.63 -1.5
691,774 - GFF1348 0.63 -0.2
691,836 + GFF1348 0.70 -0.8
691,836 + GFF1348 0.70 +0.0
691,836 + GFF1348 0.70 -0.6
691,837 - GFF1348 0.70 +0.4
691,837 - GFF1348 0.70 +0.7
691,837 - GFF1348 0.70 -0.0
691,837 - GFF1348 0.70 -0.1
692,199 + GFF1349 0.25 +1.5
692,200 - GFF1349 0.25 -1.5
692,200 - GFF1349 0.25 +0.5
692,431 - GFF1349 0.75 -0.6
692,431 - GFF1349 0.75 -0.4
692,478 + GFF1349 0.85 -1.2
692,566 + -0.7
692,567 - -1.4
692,567 - -0.5
692,697 + GFF1350 0.12 -0.5
692,697 + GFF1350 0.12 +0.1
692,697 + GFF1350 0.12 -0.5
692,697 + GFF1350 0.12 +0.3
692,697 + GFF1350 0.12 +0.7
692,697 + GFF1350 0.12 -0.3
692,698 - GFF1350 0.12 -0.3
692,698 - GFF1350 0.12 -0.0
692,698 - GFF1350 0.12 -0.2
692,698 - GFF1350 0.12 -0.5
692,698 - GFF1350 0.12 -0.3
692,698 - GFF1350 0.12 -0.3
692,698 - GFF1350 0.12 -0.6
692,698 - GFF1350 0.12 +0.3
692,698 - GFF1350 0.12 -0.3
692,715 + GFF1350 0.14 -0.4
692,715 + GFF1350 0.14 +0.4
692,715 + GFF1350 0.14 +0.5
692,716 - GFF1350 0.14 -0.2
692,716 - GFF1350 0.14 -2.1
692,748 + GFF1350 0.17 +0.2
692,748 + GFF1350 0.17 -0.9
692,749 - GFF1350 0.18 +1.6
692,826 + GFF1350 0.25 -0.5
692,826 + GFF1350 0.25 +0.1
692,826 + GFF1350 0.25 -0.1
692,827 - GFF1350 0.25 +0.1
692,827 - GFF1350 0.25 -0.0
692,827 - GFF1350 0.25 -1.5
692,827 - GFF1350 0.25 +0.7
692,827 - GFF1350 0.25 +0.8
692,871 + GFF1350 0.29 -0.8
692,871 + GFF1350 0.29 -0.7
692,872 - GFF1350 0.30 -0.4
692,872 - GFF1350 0.30 -0.6
692,983 - GFF1350 0.41 +1.5
693,132 + GFF1350 0.55 +1.0
693,132 + GFF1350 0.55 +0.2
693,133 - GFF1350 0.55 +0.7
693,133 - GFF1350 0.55 +2.6
693,133 - GFF1350 0.55 -0.4
693,133 - GFF1350 0.55 -1.5
693,264 + GFF1350 0.68 -0.6
693,264 + GFF1350 0.68 +0.3
693,265 - GFF1350 0.68 +0.5
693,265 - GFF1350 0.68 -1.0
693,265 - GFF1350 0.68 +1.3
693,509 + +0.3
693,679 + +0.5
693,679 + +1.5
693,734 + -0.4
693,734 + +0.5
693,734 + -1.5
693,734 + -1.5
693,734 + -0.3
693,735 - -0.8
693,735 - -0.2
693,944 + GFF1351 0.16 +0.4
693,944 + GFF1351 0.16 -0.6
693,945 - GFF1351 0.16 -0.3
693,945 - GFF1351 0.16 -1.9
694,094 + GFF1351 0.25 -0.8
694,094 + GFF1351 0.25 -0.7
694,095 - GFF1351 0.25 -0.5
694,095 - GFF1351 0.25 -0.2
694,134 - GFF1351 0.27 +0.4
694,134 - GFF1351 0.27 -0.3
694,310 + GFF1351 0.38 -0.5

Or see this region's nucleotide sequence