Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1241 and GFF1242 are separated by 44 nucleotides GFF1242 and GFF1243 are separated by 195 nucleotides
GFF1241 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46) periplasmic (secreted in GramPositives), at 559,964 to 561,175
GFF1241
GFF1242 - hypothetical protein, at 561,220 to 562,743
GFF1242
GFF1243 - transglutaminase, N-terminal domain protein, at 562,939 to 563,847
GFF1243
Position (kb)
561
562
563 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 560.392 kb on + strand, within GFF1241 at 560.392 kb on + strand, within GFF1241 at 560.393 kb on - strand, within GFF1241 at 560.492 kb on + strand, within GFF1241 at 560.493 kb on - strand, within GFF1241 at 560.583 kb on - strand, within GFF1241 at 560.583 kb on - strand, within GFF1241 at 560.583 kb on - strand, within GFF1241 at 560.583 kb on - strand, within GFF1241 at 560.606 kb on + strand, within GFF1241 at 560.606 kb on + strand, within GFF1241 at 560.607 kb on - strand, within GFF1241 at 560.607 kb on - strand, within GFF1241 at 560.607 kb on - strand, within GFF1241 at 560.768 kb on + strand, within GFF1241 at 560.819 kb on + strand, within GFF1241 at 560.819 kb on + strand, within GFF1241 at 560.819 kb on + strand, within GFF1241 at 560.820 kb on - strand, within GFF1241 at 560.820 kb on - strand, within GFF1241 at 560.820 kb on - strand, within GFF1241 at 560.820 kb on - strand, within GFF1241 at 560.864 kb on + strand, within GFF1241 at 560.864 kb on + strand, within GFF1241 at 560.864 kb on + strand, within GFF1241 at 560.864 kb on + strand, within GFF1241 at 560.865 kb on - strand, within GFF1241 at 560.865 kb on - strand, within GFF1241 at 560.865 kb on - strand, within GFF1241 at 561.054 kb on - strand at 561.054 kb on - strand at 561.173 kb on + strand at 561.317 kb on + strand at 561.318 kb on - strand at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.512 kb on + strand, within GFF1242 at 561.513 kb on - strand, within GFF1242 at 561.513 kb on - strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.539 kb on + strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.540 kb on - strand, within GFF1242 at 561.710 kb on + strand, within GFF1242 at 561.710 kb on + strand, within GFF1242 at 561.711 kb on - strand, within GFF1242 at 561.872 kb on + strand, within GFF1242 at 561.872 kb on + strand, within GFF1242 at 561.873 kb on - strand, within GFF1242 at 561.873 kb on - strand, within GFF1242 at 561.873 kb on - strand, within GFF1242 at 561.873 kb on - strand, within GFF1242 at 561.977 kb on + strand, within GFF1242 at 562.029 kb on - strand, within GFF1242 at 562.029 kb on - strand, within GFF1242 at 562.067 kb on - strand, within GFF1242 at 562.082 kb on + strand, within GFF1242 at 562.082 kb on + strand, within GFF1242 at 562.082 kb on + strand, within GFF1242 at 562.083 kb on - strand, within GFF1242 at 562.083 kb on - strand, within GFF1242 at 562.083 kb on - strand, within GFF1242 at 562.109 kb on + strand, within GFF1242 at 562.109 kb on + strand, within GFF1242 at 562.109 kb on + strand, within GFF1242 at 562.109 kb on + strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.110 kb on - strand, within GFF1242 at 562.271 kb on + strand, within GFF1242 at 562.271 kb on + strand, within GFF1242 at 562.272 kb on - strand, within GFF1242 at 562.272 kb on - strand, within GFF1242 at 562.274 kb on + strand, within GFF1242 at 562.274 kb on + strand, within GFF1242 at 562.274 kb on + strand, within GFF1242 at 562.274 kb on + strand, within GFF1242 at 562.274 kb on + strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.275 kb on - strand, within GFF1242 at 562.286 kb on + strand, within GFF1242 at 562.286 kb on + strand, within GFF1242 at 562.287 kb on - strand, within GFF1242 at 562.287 kb on - strand, within GFF1242 at 562.287 kb on - strand, within GFF1242 at 562.287 kb on - strand, within GFF1242 at 562.326 kb on - strand, within GFF1242 at 562.388 kb on + strand, within GFF1242 at 562.388 kb on + strand, within GFF1242 at 562.388 kb on + strand, within GFF1242 at 562.388 kb on + strand, within GFF1242 at 562.389 kb on - strand, within GFF1242 at 562.389 kb on - strand, within GFF1242 at 562.389 kb on - strand, within GFF1242 at 562.389 kb on - strand, within GFF1242 at 562.434 kb on - strand, within GFF1242 at 562.434 kb on - strand, within GFF1242 at 562.434 kb on - strand, within GFF1242 at 562.434 kb on - strand, within GFF1242 at 562.434 kb on - strand, within GFF1242 at 562.488 kb on + strand, within GFF1242 at 562.489 kb on - strand, within GFF1242 at 562.554 kb on + strand, within GFF1242 at 562.555 kb on - strand, within GFF1242 at 562.828 kb on + strand at 562.828 kb on + strand at 562.829 kb on - strand at 562.829 kb on - strand at 562.853 kb on + strand at 562.853 kb on + strand at 562.853 kb on + strand at 562.853 kb on + strand at 562.853 kb on + strand at 562.854 kb on - strand at 562.871 kb on + strand at 562.985 kb on + strand at 562.985 kb on + strand at 562.985 kb on + strand at 562.985 kb on + strand at 562.985 kb on + strand at 562.985 kb on + strand at 562.986 kb on - strand at 562.986 kb on - strand at 562.986 kb on - strand at 562.986 kb on - strand at 563.004 kb on - strand at 563.126 kb on + strand, within GFF1243 at 563.127 kb on - strand, within GFF1243 at 563.127 kb on - strand, within GFF1243 at 563.127 kb on - strand, within GFF1243 at 563.127 kb on - strand, within GFF1243 at 563.127 kb on - strand, within GFF1243 at 563.210 kb on + strand, within GFF1243 at 563.210 kb on + strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.211 kb on - strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.216 kb on + strand, within GFF1243 at 563.217 kb on - strand, within GFF1243 at 563.217 kb on - strand, within GFF1243 at 563.217 kb on - strand, within GFF1243 at 563.279 kb on + strand, within GFF1243 at 563.279 kb on + strand, within GFF1243 at 563.279 kb on + strand, within GFF1243 at 563.280 kb on - strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.354 kb on + strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.355 kb on - strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.492 kb on + strand, within GFF1243 at 563.493 kb on - strand, within GFF1243 at 563.493 kb on - strand, within GFF1243 at 563.493 kb on - strand, within GFF1243
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 560,392 + GFF1241 0.35 -1.6 560,392 + GFF1241 0.35 -0.5 560,393 - GFF1241 0.35 -1.3 560,492 + GFF1241 0.44 -0.5 560,493 - GFF1241 0.44 -0.2 560,583 - GFF1241 0.51 -0.1 560,583 - GFF1241 0.51 +0.9 560,583 - GFF1241 0.51 -0.0 560,583 - GFF1241 0.51 -0.6 560,606 + GFF1241 0.53 -1.1 560,606 + GFF1241 0.53 -0.1 560,607 - GFF1241 0.53 +0.2 560,607 - GFF1241 0.53 -0.2 560,607 - GFF1241 0.53 -0.6 560,768 + GFF1241 0.66 +0.7 560,819 + GFF1241 0.71 -0.6 560,819 + GFF1241 0.71 +0.2 560,819 + GFF1241 0.71 -1.9 560,820 - GFF1241 0.71 +0.2 560,820 - GFF1241 0.71 -1.0 560,820 - GFF1241 0.71 +0.7 560,820 - GFF1241 0.71 -0.7 560,864 + GFF1241 0.74 -0.6 560,864 + GFF1241 0.74 -0.3 560,864 + GFF1241 0.74 -0.2 560,864 + GFF1241 0.74 -1.1 560,865 - GFF1241 0.74 -0.3 560,865 - GFF1241 0.74 -0.2 560,865 - GFF1241 0.74 -0.2 561,054 - +0.2 561,054 - -1.0 561,173 + -0.6 561,317 + +0.4 561,318 - +0.5 561,512 + GFF1242 0.19 -0.4 561,512 + GFF1242 0.19 +0.0 561,512 + GFF1242 0.19 -0.1 561,512 + GFF1242 0.19 -1.1 561,512 + GFF1242 0.19 -0.3 561,512 + GFF1242 0.19 -1.1 561,512 + GFF1242 0.19 +0.3 561,512 + GFF1242 0.19 -0.4 561,513 - GFF1242 0.19 -1.6 561,513 - GFF1242 0.19 +0.3 561,539 + GFF1242 0.21 -1.3 561,539 + GFF1242 0.21 -0.1 561,539 + GFF1242 0.21 -1.2 561,539 + GFF1242 0.21 -0.6 561,539 + GFF1242 0.21 -0.8 561,539 + GFF1242 0.21 -0.3 561,539 + GFF1242 0.21 +0.3 561,539 + GFF1242 0.21 -0.5 561,540 - GFF1242 0.21 -0.4 561,540 - GFF1242 0.21 -0.4 561,540 - GFF1242 0.21 -0.4 561,540 - GFF1242 0.21 -0.4 561,540 - GFF1242 0.21 +0.1 561,540 - GFF1242 0.21 -0.5 561,710 + GFF1242 0.32 -0.3 561,710 + GFF1242 0.32 +0.7 561,711 - GFF1242 0.32 +0.5 561,872 + GFF1242 0.43 -0.0 561,872 + GFF1242 0.43 -0.2 561,873 - GFF1242 0.43 -0.4 561,873 - GFF1242 0.43 -1.7 561,873 - GFF1242 0.43 -0.1 561,873 - GFF1242 0.43 -0.3 561,977 + GFF1242 0.50 -1.1 562,029 - GFF1242 0.53 -0.4 562,029 - GFF1242 0.53 -0.1 562,067 - GFF1242 0.56 -1.5 562,082 + GFF1242 0.57 +0.0 562,082 + GFF1242 0.57 -1.3 562,082 + GFF1242 0.57 +0.1 562,083 - GFF1242 0.57 -2.0 562,083 - GFF1242 0.57 -2.3 562,083 - GFF1242 0.57 -0.3 562,109 + GFF1242 0.58 +0.1 562,109 + GFF1242 0.58 -0.3 562,109 + GFF1242 0.58 -0.7 562,109 + GFF1242 0.58 -0.8 562,110 - GFF1242 0.58 +0.3 562,110 - GFF1242 0.58 -0.3 562,110 - GFF1242 0.58 +0.0 562,110 - GFF1242 0.58 -0.6 562,110 - GFF1242 0.58 -0.1 562,110 - GFF1242 0.58 -0.0 562,110 - GFF1242 0.58 -1.5 562,271 + GFF1242 0.69 +0.1 562,271 + GFF1242 0.69 -0.5 562,272 - GFF1242 0.69 -0.9 562,272 - GFF1242 0.69 -0.3 562,274 + GFF1242 0.69 +3.6 562,274 + GFF1242 0.69 -1.7 562,274 + GFF1242 0.69 +0.1 562,274 + GFF1242 0.69 +0.3 562,274 + GFF1242 0.69 +0.3 562,275 - GFF1242 0.69 -1.0 562,275 - GFF1242 0.69 -0.6 562,275 - GFF1242 0.69 -0.1 562,275 - GFF1242 0.69 +1.0 562,275 - GFF1242 0.69 +0.6 562,275 - GFF1242 0.69 -0.9 562,275 - GFF1242 0.69 +0.2 562,286 + GFF1242 0.70 +0.5 562,286 + GFF1242 0.70 +0.8 562,287 - GFF1242 0.70 +0.4 562,287 - GFF1242 0.70 -1.3 562,287 - GFF1242 0.70 +0.2 562,287 - GFF1242 0.70 +1.0 562,326 - GFF1242 0.73 +0.2 562,388 + GFF1242 0.77 -1.8 562,388 + GFF1242 0.77 +1.9 562,388 + GFF1242 0.77 -0.3 562,388 + GFF1242 0.77 -0.3 562,389 - GFF1242 0.77 +0.2 562,389 - GFF1242 0.77 -0.5 562,389 - GFF1242 0.77 -0.2 562,389 - GFF1242 0.77 +0.1 562,434 - GFF1242 0.80 -1.2 562,434 - GFF1242 0.80 -1.0 562,434 - GFF1242 0.80 +1.3 562,434 - GFF1242 0.80 -1.0 562,434 - GFF1242 0.80 -0.1 562,488 + GFF1242 0.83 -0.3 562,489 - GFF1242 0.83 -0.0 562,554 + GFF1242 0.88 +0.2 562,555 - GFF1242 0.88 +0.1 562,828 + -0.4 562,828 + +0.9 562,829 - -0.0 562,829 - -0.4 562,853 + +0.3 562,853 + -0.6 562,853 + -2.3 562,853 + -1.0 562,853 + +0.6 562,854 - +0.7 562,871 + +2.3 562,985 + -1.1 562,985 + +0.5 562,985 + +0.3 562,985 + -0.5 562,985 + +0.7 562,985 + +0.1 562,986 - -1.7 562,986 - -0.3 562,986 - +1.5 562,986 - +0.4 563,004 - -0.1 563,126 + GFF1243 0.21 -1.7 563,127 - GFF1243 0.21 -0.0 563,127 - GFF1243 0.21 -1.0 563,127 - GFF1243 0.21 -1.5 563,127 - GFF1243 0.21 +0.1 563,127 - GFF1243 0.21 -0.8 563,210 + GFF1243 0.30 +0.2 563,210 + GFF1243 0.30 +0.5 563,211 - GFF1243 0.30 +0.1 563,211 - GFF1243 0.30 -0.1 563,211 - GFF1243 0.30 -1.7 563,211 - GFF1243 0.30 +0.1 563,211 - GFF1243 0.30 +0.2 563,211 - GFF1243 0.30 -0.4 563,211 - GFF1243 0.30 +0.5 563,216 + GFF1243 0.30 -0.0 563,216 + GFF1243 0.30 -0.4 563,216 + GFF1243 0.30 -0.3 563,216 + GFF1243 0.30 -0.2 563,216 + GFF1243 0.30 -0.6 563,216 + GFF1243 0.30 -0.3 563,217 - GFF1243 0.31 -0.3 563,217 - GFF1243 0.31 -0.6 563,217 - GFF1243 0.31 +0.1 563,279 + GFF1243 0.37 -0.2 563,279 + GFF1243 0.37 -0.5 563,279 + GFF1243 0.37 -0.7 563,280 - GFF1243 0.38 -0.4 563,354 + GFF1243 0.46 -0.7 563,354 + GFF1243 0.46 +0.6 563,354 + GFF1243 0.46 +2.3 563,354 + GFF1243 0.46 +0.3 563,355 - GFF1243 0.46 +0.3 563,355 - GFF1243 0.46 -0.6 563,355 - GFF1243 0.46 -0.6 563,355 - GFF1243 0.46 -0.3 563,355 - GFF1243 0.46 +0.5 563,492 + GFF1243 0.61 -1.0 563,492 + GFF1243 0.61 +0.1 563,492 + GFF1243 0.61 +0.1 563,493 - GFF1243 0.61 -1.2 563,493 - GFF1243 0.61 +1.1 563,493 - GFF1243 0.61 -0.0
Or see this region's nucleotide sequence