Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1202 and GFF1203 overlap by 1 nucleotides GFF1203 and GFF1204 are separated by 47 nucleotides GFF1204 and GFF1205 are separated by 21 nucleotides
GFF1202 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4), at 520,122 to 521,459
GFF1202
GFF1203 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 521,459 to 522,400
GFF1203
GFF1204 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8), at 522,448 to 523,068
GFF1204
GFF1205 - Oxidoreductase probably involved in sulfite reduction, at 523,090 to 523,485
GFF1205
Position (kb) 521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 520.795 kb on + strand, within GFF1202 at 520.796 kb on - strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.146 kb on + strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.147 kb on - strand, within GFF1202 at 521.203 kb on + strand, within GFF1202 at 521.204 kb on - strand, within GFF1202 at 521.209 kb on + strand, within GFF1202 at 521.441 kb on + strand at 521.441 kb on + strand at 521.442 kb on - strand at 521.442 kb on - strand at 521.442 kb on - strand at 521.466 kb on + strand at 521.467 kb on - strand at 521.654 kb on + strand, within GFF1203 at 521.739 kb on + strand, within GFF1203 at 521.739 kb on + strand, within GFF1203 at 521.740 kb on - strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.742 kb on + strand, within GFF1203 at 521.743 kb on - strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.778 kb on + strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 521.779 kb on - strand, within GFF1203 at 522.141 kb on + strand, within GFF1203 at 522.506 kb on + strand at 522.506 kb on + strand at 522.506 kb on + strand at 522.506 kb on + strand at 522.506 kb on + strand at 522.507 kb on - strand at 522.507 kb on - strand at 522.542 kb on + strand, within GFF1204 at 522.543 kb on - strand, within GFF1204 at 522.812 kb on + strand, within GFF1204 at 522.812 kb on + strand, within GFF1204 at 522.813 kb on - strand, within GFF1204 at 522.813 kb on - strand, within GFF1204 at 522.813 kb on - strand, within GFF1204 at 522.813 kb on - strand, within GFF1204 at 522.863 kb on + strand, within GFF1204 at 522.864 kb on - strand, within GFF1204 at 522.864 kb on - strand, within GFF1204 at 522.864 kb on - strand, within GFF1204 at 522.864 kb on - strand, within GFF1204 at 522.881 kb on + strand, within GFF1204 at 522.881 kb on + strand, within GFF1204 at 522.881 kb on + strand, within GFF1204 at 522.882 kb on - strand, within GFF1204 at 523.076 kb on + strand at 523.076 kb on + strand at 523.077 kb on - strand at 523.139 kb on + strand, within GFF1205 at 523.139 kb on + strand, within GFF1205 at 523.139 kb on + strand, within GFF1205 at 523.139 kb on + strand, within GFF1205 at 523.139 kb on + strand, within GFF1205 at 523.139 kb on + strand, within GFF1205 at 523.140 kb on - strand, within GFF1205 at 523.140 kb on - strand, within GFF1205
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 520,795 + GFF1202 0.50 -0.1 520,796 - GFF1202 0.50 -0.9 521,146 + GFF1202 0.77 -1.7 521,146 + GFF1202 0.77 +1.1 521,146 + GFF1202 0.77 -0.9 521,146 + GFF1202 0.77 -3.1 521,147 - GFF1202 0.77 -0.2 521,147 - GFF1202 0.77 +0.6 521,147 - GFF1202 0.77 -2.0 521,147 - GFF1202 0.77 -0.8 521,147 - GFF1202 0.77 +0.2 521,203 + GFF1202 0.81 -0.8 521,204 - GFF1202 0.81 -1.0 521,209 + GFF1202 0.81 -0.7 521,441 + -1.3 521,441 + -1.4 521,442 - +0.0 521,442 - -0.6 521,442 - -0.1 521,466 + -0.6 521,467 - -1.0 521,654 + GFF1203 0.21 -1.6 521,739 + GFF1203 0.30 -1.2 521,739 + GFF1203 0.30 -0.2 521,740 - GFF1203 0.30 -2.1 521,742 + GFF1203 0.30 -2.4 521,742 + GFF1203 0.30 -0.7 521,742 + GFF1203 0.30 -2.4 521,743 - GFF1203 0.30 -0.4 521,778 + GFF1203 0.34 -1.5 521,778 + GFF1203 0.34 +0.2 521,778 + GFF1203 0.34 -1.1 521,778 + GFF1203 0.34 +0.3 521,778 + GFF1203 0.34 -1.0 521,778 + GFF1203 0.34 -0.9 521,779 - GFF1203 0.34 -1.4 521,779 - GFF1203 0.34 -0.1 521,779 - GFF1203 0.34 -0.3 521,779 - GFF1203 0.34 -0.5 521,779 - GFF1203 0.34 -0.7 521,779 - GFF1203 0.34 -1.9 521,779 - GFF1203 0.34 -2.1 521,779 - GFF1203 0.34 -1.3 521,779 - GFF1203 0.34 -0.6 522,141 + GFF1203 0.72 -3.3 522,506 + -1.6 522,506 + +0.3 522,506 + -1.7 522,506 + -2.2 522,506 + -1.0 522,507 - -0.1 522,507 - -0.1 522,542 + GFF1204 0.15 -0.7 522,543 - GFF1204 0.15 -2.3 522,812 + GFF1204 0.59 -1.1 522,812 + GFF1204 0.59 -2.3 522,813 - GFF1204 0.59 +0.5 522,813 - GFF1204 0.59 -1.3 522,813 - GFF1204 0.59 -1.3 522,813 - GFF1204 0.59 -0.9 522,863 + GFF1204 0.67 -1.0 522,864 - GFF1204 0.67 -0.7 522,864 - GFF1204 0.67 -1.0 522,864 - GFF1204 0.67 -1.0 522,864 - GFF1204 0.67 -1.3 522,881 + GFF1204 0.70 -0.7 522,881 + GFF1204 0.70 -0.8 522,881 + GFF1204 0.70 -0.7 522,882 - GFF1204 0.70 -1.2 523,076 + +0.3 523,076 + -0.3 523,077 - +3.3 523,139 + GFF1205 0.12 -1.9 523,139 + GFF1205 0.12 -0.4 523,139 + GFF1205 0.12 -0.3 523,139 + GFF1205 0.12 -2.0 523,139 + GFF1205 0.12 -1.4 523,139 + GFF1205 0.12 -1.9 523,140 - GFF1205 0.13 -1.0 523,140 - GFF1205 0.13 -1.6
Or see this region's nucleotide sequence