Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF107 and GFF108 are separated by 68 nucleotides GFF108 and GFF109 are separated by 141 nucleotides GFF109 and GFF110 are separated by 10 nucleotides GFF110 and GFF111 overlap by 4 nucleotides
GFF107 - General secretion pathway protein K, at 134,887 to 135,846
GFF107
GFF108 - General secretion pathway protein G, at 135,915 to 136,385
GFF108
GFF109 - Protein lysine acetyltransferase Pat (EC 2.3.1.-), at 136,527 to 139,229
GFF109
GFF110 - pyridoxal-phosphate dependent enzyme family protein, at 139,240 to 140,199
GFF110
GFF111 - hypothetical protein, at 140,196 to 141,221
GFF111
Position (kb)
136
137
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 135.623 kb on + strand, within GFF107 at 135.623 kb on + strand, within GFF107 at 136.015 kb on + strand, within GFF108 at 136.016 kb on - strand, within GFF108 at 136.016 kb on - strand, within GFF108 at 136.021 kb on + strand, within GFF108 at 136.138 kb on + strand, within GFF108 at 136.139 kb on - strand, within GFF108 at 137.142 kb on + strand, within GFF109 at 137.154 kb on - strand, within GFF109 at 137.850 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.180 kb on + strand, within GFF109 at 138.181 kb on - strand, within GFF109 at 138.181 kb on - strand, within GFF109 at 138.181 kb on - strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.879 kb on + strand, within GFF109 at 138.978 kb on + strand at 138.978 kb on + strand at 138.979 kb on - strand at 138.979 kb on - strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.227 kb on + strand at 139.442 kb on + strand, within GFF110 at 139.442 kb on + strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.554 kb on - strand, within GFF110 at 139.745 kb on + strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.929 kb on - strand, within GFF110 at 140.121 kb on + strand at 140.132 kb on + strand at 140.132 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 135,623 + GFF107 0.77 +0.2 135,623 + GFF107 0.77 -1.5 136,015 + GFF108 0.21 +1.2 136,016 - GFF108 0.21 -0.5 136,016 - GFF108 0.21 +0.3 136,021 + GFF108 0.23 -0.5 136,138 + GFF108 0.47 -1.1 136,139 - GFF108 0.48 +0.1 137,142 + GFF109 0.23 -0.3 137,154 - GFF109 0.23 +0.3 137,850 + GFF109 0.49 -0.6 138,180 + GFF109 0.61 -2.6 138,180 + GFF109 0.61 +0.0 138,180 + GFF109 0.61 +0.5 138,180 + GFF109 0.61 -0.1 138,180 + GFF109 0.61 -0.3 138,180 + GFF109 0.61 +1.2 138,181 - GFF109 0.61 -2.6 138,181 - GFF109 0.61 -0.4 138,181 - GFF109 0.61 -0.1 138,280 + GFF109 0.65 +0.9 138,280 + GFF109 0.65 +0.1 138,280 + GFF109 0.65 +0.6 138,280 + GFF109 0.65 -0.2 138,280 + GFF109 0.65 +0.2 138,281 - GFF109 0.65 +0.1 138,281 - GFF109 0.65 -1.0 138,281 - GFF109 0.65 -0.6 138,282 + GFF109 0.65 -1.6 138,282 + GFF109 0.65 -0.0 138,282 + GFF109 0.65 -0.9 138,282 + GFF109 0.65 -0.1 138,282 + GFF109 0.65 +0.2 138,283 - GFF109 0.65 +0.7 138,283 - GFF109 0.65 +0.3 138,732 + GFF109 0.82 -1.3 138,732 + GFF109 0.82 +1.7 138,732 + GFF109 0.82 -0.5 138,732 + GFF109 0.82 -0.2 138,732 + GFF109 0.82 +0.4 138,733 - GFF109 0.82 -0.2 138,733 - GFF109 0.82 -0.5 138,733 - GFF109 0.82 -0.1 138,879 + GFF109 0.87 +2.7 138,978 + +0.8 138,978 + +0.2 138,979 - -0.8 138,979 - -0.6 139,218 + +0.2 139,218 + +0.0 139,218 + +0.5 139,218 + -0.9 139,218 + -0.4 139,219 - +0.0 139,219 - +0.7 139,219 - +1.2 139,219 - +0.6 139,219 - -0.5 139,227 + -0.4 139,442 + GFF110 0.21 -0.4 139,442 + GFF110 0.21 -0.6 139,443 - GFF110 0.21 +1.0 139,443 - GFF110 0.21 -0.2 139,553 + GFF110 0.33 +0.4 139,553 + GFF110 0.33 -1.0 139,553 + GFF110 0.33 -0.5 139,553 + GFF110 0.33 +1.2 139,554 - GFF110 0.33 +0.6 139,745 + GFF110 0.53 -0.7 139,746 - GFF110 0.53 +0.0 139,746 - GFF110 0.53 -0.1 139,746 - GFF110 0.53 +0.0 139,746 - GFF110 0.53 -0.8 139,928 + GFF110 0.72 +0.1 139,928 + GFF110 0.72 +0.0 139,928 + GFF110 0.72 +1.2 139,929 - GFF110 0.72 +0.1 140,121 + +0.8 140,132 + -1.6 140,132 + +0.7
Or see this region's nucleotide sequence