Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1012 and GFF1013 are separated by 114 nucleotides GFF1013 and GFF1014 are separated by 58 nucleotides GFF1014 and GFF1015 are separated by 36 nucleotides
GFF1012 - Cystathionine beta-lyase (EC 4.4.1.8), at 331,431 to 332,651
GFF1012
GFF1013 - hypothetical protein, at 332,766 to 332,969
GFF1013
GFF1014 - Arylesterase precursor (EC 3.1.1.2), at 333,028 to 333,666
GFF1014
GFF1015 - ABC-type antimicrobial peptide transport system, ATPase component, at 333,703 to 334,413
GFF1015
Position (kb) 332
333 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 331.779 kb on + strand, within GFF1012 at 331.780 kb on - strand, within GFF1012 at 331.842 kb on + strand, within GFF1012 at 331.842 kb on + strand, within GFF1012 at 331.843 kb on - strand, within GFF1012 at 331.843 kb on - strand, within GFF1012 at 331.843 kb on - strand, within GFF1012 at 331.843 kb on - strand, within GFF1012 at 331.932 kb on + strand, within GFF1012 at 332.089 kb on - strand, within GFF1012 at 332.089 kb on - strand, within GFF1012 at 332.089 kb on - strand, within GFF1012 at 332.089 kb on - strand, within GFF1012 at 332.247 kb on + strand, within GFF1012 at 332.247 kb on + strand, within GFF1012 at 332.247 kb on + strand, within GFF1012 at 332.247 kb on + strand, within GFF1012 at 332.248 kb on - strand, within GFF1012 at 332.248 kb on - strand, within GFF1012 at 332.378 kb on + strand, within GFF1012 at 332.379 kb on - strand, within GFF1012 at 332.379 kb on - strand, within GFF1012 at 332.517 kb on + strand, within GFF1012 at 332.517 kb on + strand, within GFF1012 at 332.518 kb on - strand, within GFF1012 at 332.518 kb on - strand, within GFF1012 at 332.650 kb on - strand at 332.650 kb on - strand at 332.650 kb on - strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.717 kb on - strand at 332.721 kb on + strand at 332.721 kb on + strand at 332.721 kb on + strand at 332.721 kb on + strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.722 kb on - strand at 332.781 kb on + strand at 332.781 kb on + strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.782 kb on - strand at 332.787 kb on + strand, within GFF1013 at 332.788 kb on - strand, within GFF1013 at 333.231 kb on - strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.242 kb on + strand, within GFF1014 at 333.243 kb on - strand, within GFF1014 at 333.243 kb on - strand, within GFF1014 at 333.243 kb on - strand, within GFF1014 at 333.243 kb on - strand, within GFF1014 at 333.243 kb on - strand, within GFF1014 at 333.261 kb on + strand, within GFF1014 at 333.518 kb on - strand, within GFF1014 at 333.545 kb on + strand, within GFF1014 at 333.545 kb on + strand, within GFF1014 at 333.546 kb on - strand, within GFF1014 at 333.546 kb on - strand, within GFF1014 at 333.546 kb on - strand, within GFF1014 at 333.546 kb on - strand, within GFF1014 at 333.546 kb on - strand, within GFF1014 at 333.669 kb on + strand at 333.669 kb on + strand at 333.669 kb on + strand at 333.669 kb on + strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand at 333.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 331,779 + GFF1012 0.29 -1.2 331,780 - GFF1012 0.29 +0.7 331,842 + GFF1012 0.34 +0.4 331,842 + GFF1012 0.34 -0.7 331,843 - GFF1012 0.34 +1.1 331,843 - GFF1012 0.34 -0.2 331,843 - GFF1012 0.34 -0.4 331,843 - GFF1012 0.34 -1.2 331,932 + GFF1012 0.41 +0.1 332,089 - GFF1012 0.54 +0.2 332,089 - GFF1012 0.54 -0.5 332,089 - GFF1012 0.54 -0.9 332,089 - GFF1012 0.54 -0.4 332,247 + GFF1012 0.67 -0.6 332,247 + GFF1012 0.67 -0.5 332,247 + GFF1012 0.67 -0.8 332,247 + GFF1012 0.67 -0.1 332,248 - GFF1012 0.67 -0.4 332,248 - GFF1012 0.67 -0.5 332,378 + GFF1012 0.78 -0.5 332,379 - GFF1012 0.78 +0.4 332,379 - GFF1012 0.78 -2.9 332,517 + GFF1012 0.89 +0.4 332,517 + GFF1012 0.89 -0.2 332,518 - GFF1012 0.89 -0.5 332,518 - GFF1012 0.89 +0.6 332,650 - -0.2 332,650 - -0.1 332,650 - +0.4 332,716 + -0.0 332,716 + -0.9 332,716 + +0.4 332,716 + +0.6 332,716 + -0.2 332,716 + +1.9 332,716 + -0.6 332,717 - -1.1 332,717 - +0.3 332,717 - +0.5 332,717 - -0.7 332,717 - -0.6 332,717 - -0.8 332,717 - +1.6 332,721 + +0.5 332,721 + +0.1 332,721 + -1.0 332,721 + -0.0 332,722 - -1.0 332,722 - +0.4 332,722 - -0.9 332,722 - -0.1 332,722 - -0.6 332,722 - -1.0 332,722 - -0.5 332,781 + +0.1 332,781 + +0.4 332,782 - +0.7 332,782 - -0.4 332,782 - -0.8 332,782 - +0.2 332,782 - -0.3 332,782 - -0.9 332,782 - -1.2 332,787 + GFF1013 0.10 -0.8 332,788 - GFF1013 0.11 -1.0 333,231 - GFF1014 0.32 +0.9 333,242 + GFF1014 0.33 +0.0 333,242 + GFF1014 0.33 +0.1 333,242 + GFF1014 0.33 -1.0 333,242 + GFF1014 0.33 -1.0 333,242 + GFF1014 0.33 -0.2 333,242 + GFF1014 0.33 +0.8 333,243 - GFF1014 0.34 -0.8 333,243 - GFF1014 0.34 -0.4 333,243 - GFF1014 0.34 +0.0 333,243 - GFF1014 0.34 -1.3 333,243 - GFF1014 0.34 -0.6 333,261 + GFF1014 0.36 -0.3 333,518 - GFF1014 0.77 +0.6 333,545 + GFF1014 0.81 -0.1 333,545 + GFF1014 0.81 -0.4 333,546 - GFF1014 0.81 -1.0 333,546 - GFF1014 0.81 +0.6 333,546 - GFF1014 0.81 +1.1 333,546 - GFF1014 0.81 +1.6 333,546 - GFF1014 0.81 -0.6 333,669 + -0.8 333,669 + +0.2 333,669 + +0.4 333,669 + -1.6 333,670 - +0.5 333,670 - -0.4 333,670 - -0.8 333,670 - -0.3 333,670 - +0.3 333,670 - -0.2 333,670 - +0.6 333,670 - -2.0 333,670 - +0.0 333,670 - +0.4
Or see this region's nucleotide sequence