Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF992 and GFF993 are separated by 4 nucleotides GFF993 and GFF994 overlap by 8 nucleotides GFF994 and GFF995 overlap by 4 nucleotides
GFF992 - 23S rRNA (adenine(2503)-C(2))-methyltransferase @ tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192), at 310,994 to 312,142
GFF992
GFF993 - Type IV pilus biogenesis protein PilF, at 312,147 to 313,001
GFF993
GFF994 - Cytoskeleton protein RodZ, at 312,994 to 313,959
GFF994
GFF995 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (EC 1.17.7.3), at 313,956 to 315,230
GFF995
Position (kb) 312
313
314 Strain fitness (log2 ratio) -2
-1
0
1 at 312.373 kb on - strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.453 kb on + strand, within GFF993 at 312.454 kb on - strand, within GFF993 at 312.454 kb on - strand, within GFF993 at 312.454 kb on - strand, within GFF993 at 312.454 kb on - strand, within GFF993 at 312.454 kb on - strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.474 kb on + strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.475 kb on - strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.564 kb on + strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.565 kb on - strand, within GFF993 at 312.642 kb on + strand, within GFF993 at 312.642 kb on + strand, within GFF993 at 312.642 kb on + strand, within GFF993 at 312.643 kb on - strand, within GFF993 at 312.735 kb on + strand, within GFF993 at 312.735 kb on + strand, within GFF993 at 312.735 kb on + strand, within GFF993 at 312.736 kb on - strand, within GFF993 at 312.736 kb on - strand, within GFF993 at 312.736 kb on - strand, within GFF993 at 312.736 kb on - strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.822 kb on + strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.823 kb on - strand, within GFF993 at 312.975 kb on + strand at 312.975 kb on + strand at 312.975 kb on + strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 312.976 kb on - strand at 313.202 kb on + strand, within GFF994 at 313.203 kb on - strand, within GFF994 at 313.596 kb on - strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.625 kb on + strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994 at 313.626 kb on - strand, within GFF994
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 312,373 - GFF993 0.26 +1.1 312,453 + GFF993 0.36 +0.4 312,453 + GFF993 0.36 -1.2 312,453 + GFF993 0.36 -0.5 312,453 + GFF993 0.36 -1.4 312,453 + GFF993 0.36 -0.9 312,453 + GFF993 0.36 -0.5 312,453 + GFF993 0.36 -0.3 312,453 + GFF993 0.36 -0.4 312,454 - GFF993 0.36 +0.3 312,454 - GFF993 0.36 +0.4 312,454 - GFF993 0.36 -1.5 312,454 - GFF993 0.36 -0.1 312,454 - GFF993 0.36 -0.1 312,474 + GFF993 0.38 -1.2 312,474 + GFF993 0.38 -1.6 312,474 + GFF993 0.38 -1.5 312,474 + GFF993 0.38 -1.7 312,474 + GFF993 0.38 -1.1 312,474 + GFF993 0.38 -1.7 312,474 + GFF993 0.38 -1.0 312,475 - GFF993 0.38 -0.4 312,475 - GFF993 0.38 -0.4 312,475 - GFF993 0.38 -0.6 312,475 - GFF993 0.38 -0.7 312,475 - GFF993 0.38 -1.1 312,475 - GFF993 0.38 -1.6 312,475 - GFF993 0.38 -1.7 312,475 - GFF993 0.38 -0.6 312,475 - GFF993 0.38 -1.1 312,564 + GFF993 0.49 -0.8 312,564 + GFF993 0.49 -0.6 312,564 + GFF993 0.49 -0.6 312,564 + GFF993 0.49 -0.1 312,564 + GFF993 0.49 -1.4 312,564 + GFF993 0.49 -0.2 312,564 + GFF993 0.49 -0.1 312,564 + GFF993 0.49 -0.7 312,564 + GFF993 0.49 +0.0 312,564 + GFF993 0.49 +1.4 312,564 + GFF993 0.49 -0.1 312,564 + GFF993 0.49 -2.0 312,564 + GFF993 0.49 -0.0 312,564 + GFF993 0.49 -0.2 312,564 + GFF993 0.49 -0.5 312,564 + GFF993 0.49 -0.0 312,565 - GFF993 0.49 -2.1 312,565 - GFF993 0.49 -0.1 312,565 - GFF993 0.49 -0.6 312,565 - GFF993 0.49 -1.1 312,565 - GFF993 0.49 -0.5 312,565 - GFF993 0.49 -0.6 312,565 - GFF993 0.49 -1.6 312,565 - GFF993 0.49 -1.0 312,565 - GFF993 0.49 -1.3 312,565 - GFF993 0.49 -0.2 312,565 - GFF993 0.49 -0.2 312,565 - GFF993 0.49 -0.4 312,565 - GFF993 0.49 -0.2 312,565 - GFF993 0.49 -0.1 312,565 - GFF993 0.49 -1.1 312,565 - GFF993 0.49 -0.6 312,642 + GFF993 0.58 -0.0 312,642 + GFF993 0.58 -0.9 312,642 + GFF993 0.58 -1.1 312,643 - GFF993 0.58 -1.6 312,735 + GFF993 0.69 -0.9 312,735 + GFF993 0.69 +0.9 312,735 + GFF993 0.69 -1.0 312,736 - GFF993 0.69 -0.1 312,736 - GFF993 0.69 -1.0 312,736 - GFF993 0.69 -0.4 312,736 - GFF993 0.69 +0.3 312,822 + GFF993 0.79 -2.1 312,822 + GFF993 0.79 -0.9 312,822 + GFF993 0.79 -1.3 312,822 + GFF993 0.79 -0.7 312,822 + GFF993 0.79 -0.8 312,822 + GFF993 0.79 -0.3 312,822 + GFF993 0.79 -1.0 312,822 + GFF993 0.79 -0.6 312,822 + GFF993 0.79 -1.7 312,822 + GFF993 0.79 -1.3 312,823 - GFF993 0.79 -0.9 312,823 - GFF993 0.79 -1.8 312,823 - GFF993 0.79 -2.0 312,823 - GFF993 0.79 -1.5 312,823 - GFF993 0.79 -1.1 312,823 - GFF993 0.79 -0.1 312,975 + -0.6 312,975 + -0.0 312,975 + +0.3 312,976 - -1.9 312,976 - -1.0 312,976 - -1.0 312,976 - -0.0 312,976 - -1.1 313,202 + GFF994 0.22 -1.1 313,203 - GFF994 0.22 -0.8 313,596 - GFF994 0.62 -0.4 313,625 + GFF994 0.65 -0.1 313,625 + GFF994 0.65 -1.5 313,625 + GFF994 0.65 -0.2 313,625 + GFF994 0.65 -0.8 313,625 + GFF994 0.65 +0.0 313,625 + GFF994 0.65 -0.2 313,626 - GFF994 0.65 -2.0 313,626 - GFF994 0.65 -0.1 313,626 - GFF994 0.65 -1.9 313,626 - GFF994 0.65 +0.4 313,626 - GFF994 0.65 -0.7 313,626 - GFF994 0.65 -0.9
Or see this region's nucleotide sequence