Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF912 and GFF913 overlap by 4 nucleotides GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 1 nucleotides
GFF912 - putative acetyl esterase, at 223,061 to 224,053
GFF912
GFF913 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 224,050 to 225,714
GFF913
GFF914 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 225,711 to 226,526
GFF914
GFF915 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 226,528 to 227,472
GFF915
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 223.078 kb on + strand at 223.079 kb on - strand at 223.091 kb on + strand at 223.091 kb on + strand at 223.092 kb on - strand at 223.351 kb on - strand, within GFF912 at 223.434 kb on + strand, within GFF912 at 223.435 kb on - strand, within GFF912 at 223.435 kb on - strand, within GFF912 at 223.443 kb on + strand, within GFF912 at 223.444 kb on - strand, within GFF912 at 223.633 kb on + strand, within GFF912 at 223.653 kb on + strand, within GFF912 at 223.653 kb on + strand, within GFF912 at 223.654 kb on - strand, within GFF912 at 223.654 kb on - strand, within GFF912 at 223.755 kb on + strand, within GFF912 at 223.756 kb on - strand, within GFF912 at 223.756 kb on - strand, within GFF912 at 223.791 kb on + strand, within GFF912 at 223.792 kb on - strand, within GFF912 at 223.792 kb on - strand, within GFF912 at 223.920 kb on + strand, within GFF912 at 224.029 kb on - strand at 224.138 kb on + strand at 224.139 kb on - strand at 224.139 kb on - strand at 224.870 kb on - strand, within GFF913 at 224.948 kb on + strand, within GFF913 at 224.948 kb on + strand, within GFF913 at 224.949 kb on - strand, within GFF913 at 224.949 kb on - strand, within GFF913 at 225.116 kb on - strand, within GFF913 at 225.246 kb on - strand, within GFF913 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.835 kb on + strand, within GFF914 at 225.836 kb on - strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.003 kb on + strand, within GFF914 at 226.004 kb on - strand, within GFF914 at 226.042 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.102 kb on + strand, within GFF914 at 226.103 kb on - strand, within GFF914 at 226.435 kb on + strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.436 kb on - strand, within GFF914 at 226.505 kb on + strand at 226.529 kb on + strand at 226.529 kb on + strand at 226.562 kb on + strand at 226.563 kb on - strand at 226.595 kb on + strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand at 226.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 223,078 + -1.1 223,079 - -0.8 223,091 + +0.5 223,091 + +0.0 223,092 - -0.3 223,351 - GFF912 0.29 -0.6 223,434 + GFF912 0.38 -0.7 223,435 - GFF912 0.38 -1.5 223,435 - GFF912 0.38 -0.3 223,443 + GFF912 0.38 -0.4 223,444 - GFF912 0.39 -1.7 223,633 + GFF912 0.58 +0.1 223,653 + GFF912 0.60 -0.3 223,653 + GFF912 0.60 +0.6 223,654 - GFF912 0.60 -1.0 223,654 - GFF912 0.60 -0.6 223,755 + GFF912 0.70 +0.7 223,756 - GFF912 0.70 +0.5 223,756 - GFF912 0.70 -0.0 223,791 + GFF912 0.74 -1.7 223,792 - GFF912 0.74 -0.4 223,792 - GFF912 0.74 -2.0 223,920 + GFF912 0.87 +0.5 224,029 - +0.0 224,138 + +0.5 224,139 - +0.5 224,139 - -0.8 224,870 - GFF913 0.49 -0.1 224,948 + GFF913 0.54 -0.3 224,948 + GFF913 0.54 +2.5 224,949 - GFF913 0.54 -0.1 224,949 - GFF913 0.54 +1.1 225,116 - GFF913 0.64 -1.1 225,246 - GFF913 0.72 +1.7 225,835 + GFF914 0.15 +0.3 225,835 + GFF914 0.15 -0.7 225,835 + GFF914 0.15 -0.2 225,836 - GFF914 0.15 -0.3 226,003 + GFF914 0.36 -1.1 226,003 + GFF914 0.36 -1.5 226,004 - GFF914 0.36 -0.2 226,042 + GFF914 0.41 -1.1 226,102 + GFF914 0.48 +0.0 226,102 + GFF914 0.48 -1.4 226,103 - GFF914 0.48 +0.9 226,435 + GFF914 0.89 -1.2 226,436 - GFF914 0.89 -0.5 226,436 - GFF914 0.89 -0.0 226,436 - GFF914 0.89 +0.9 226,436 - GFF914 0.89 -0.3 226,436 - GFF914 0.89 -1.1 226,436 - GFF914 0.89 -0.4 226,436 - GFF914 0.89 +0.1 226,436 - GFF914 0.89 -0.7 226,505 + +3.3 226,529 + -0.3 226,529 + +0.7 226,562 + -1.0 226,563 - -0.1 226,595 + +0.5 226,596 - -1.1 226,596 - +0.2 226,596 - +0.3 226,596 - -0.1
Or see this region's nucleotide sequence