Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF76 and GFF77 overlap by 7 nucleotides GFF77 and GFF78 are separated by 1 nucleotides GFF78 and GFF79 are separated by 72 nucleotides GFF79 and GFF80 are separated by 42 nucleotides
GFF76 - Alkanesulfonates transport system permease protein, at 93,161 to 94,228
GFF76
GFF77 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 94,222 to 95,025
GFF77
GFF78 - Cytochrome c family protein, at 95,027 to 96,190
GFF78
GFF79 - Dodecin, a flavin storage/sequestration protein, at 96,263 to 96,469
GFF79
GFF80 - no description, at 96,512 to 98,155
GFF80
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.080 kb on - strand, within GFF76 at 94.080 kb on - strand, within GFF76 at 94.109 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.147 kb on - strand at 94.534 kb on + strand, within GFF77 at 94.534 kb on + strand, within GFF77 at 94.535 kb on - strand, within GFF77 at 94.535 kb on - strand, within GFF77 at 95.439 kb on - strand, within GFF78 at 96.206 kb on + strand at 96.206 kb on + strand at 96.278 kb on + strand at 96.278 kb on + strand at 96.279 kb on - strand at 96.279 kb on - strand at 96.279 kb on - strand at 96.458 kb on - strand at 96.540 kb on + strand at 96.570 kb on + strand at 96.570 kb on + strand at 96.571 kb on - strand at 96.624 kb on - strand at 96.672 kb on + strand at 96.673 kb on - strand at 96.701 kb on + strand, within GFF80 at 96.702 kb on - strand, within GFF80 at 96.846 kb on + strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.847 kb on - strand, within GFF80 at 96.909 kb on + strand, within GFF80 at 96.910 kb on - strand, within GFF80 at 96.910 kb on - strand, within GFF80 at 97.039 kb on - strand, within GFF80
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 94,080 - GFF76 0.86 +0.2 94,080 - GFF76 0.86 -0.3 94,109 + GFF76 0.89 +0.5 94,121 + GFF76 0.90 +0.1 94,121 + GFF76 0.90 +1.3 94,121 + GFF76 0.90 -0.8 94,121 + GFF76 0.90 -0.9 94,121 + GFF76 0.90 +0.1 94,122 - -2.8 94,122 - -0.9 94,122 - +0.1 94,122 - -0.0 94,147 - +0.7 94,534 + GFF77 0.39 -0.8 94,534 + GFF77 0.39 +0.1 94,535 - GFF77 0.39 -0.9 94,535 - GFF77 0.39 -2.1 95,439 - GFF78 0.35 +0.3 96,206 + -1.3 96,206 + -1.9 96,278 + +0.2 96,278 + -0.0 96,279 - +0.2 96,279 - +2.3 96,279 - -0.5 96,458 - -0.2 96,540 + +0.5 96,570 + -1.1 96,570 + +0.4 96,571 - -1.3 96,624 - +0.2 96,672 + +0.2 96,673 - +1.3 96,701 + GFF80 0.11 -0.1 96,702 - GFF80 0.12 -0.1 96,846 + GFF80 0.20 -0.2 96,847 - GFF80 0.20 +0.2 96,847 - GFF80 0.20 +0.0 96,847 - GFF80 0.20 -1.3 96,909 + GFF80 0.24 -1.3 96,910 - GFF80 0.24 -1.5 96,910 - GFF80 0.24 -0.0 97,039 - GFF80 0.32 +0.9
Or see this region's nucleotide sequence