Strain Fitness in Variovorax sp. SCN45 around GFF757

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF755 and GFF756 overlap by 4 nucleotidesGFF756 and GFF757 overlap by 4 nucleotidesGFF757 and GFF758 are separated by 9 nucleotidesGFF758 and GFF759 are separated by 13 nucleotides GFF755 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 18,670 to 20,568 GFF755 GFF756 - no description, at 20,565 to 20,711 GFF756 GFF757 - hypothetical protein, at 20,708 to 21,535 GFF757 GFF758 - 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55), at 21,545 to 22,390 GFF758 GFF759 - FIG01121861: hypothetical protein, at 22,404 to 23,597 GFF759 Position (kb) 20 21 22Strain fitness (log2 ratio) -3 -2 -1 0 1at 19.835 kb on + strand, within GFF755at 19.835 kb on + strand, within GFF755at 19.835 kb on + strand, within GFF755at 19.835 kb on + strand, within GFF755at 19.836 kb on - strand, within GFF755at 19.836 kb on - strand, within GFF755at 19.836 kb on - strand, within GFF755at 20.224 kb on + strand, within GFF755at 20.224 kb on + strand, within GFF755at 20.225 kb on - strand, within GFF755at 20.225 kb on - strand, within GFF755at 20.302 kb on + strand, within GFF755at 20.329 kb on + strand, within GFF755at 20.329 kb on + strand, within GFF755at 20.329 kb on + strand, within GFF755at 20.330 kb on - strand, within GFF755at 20.330 kb on - strand, within GFF755at 20.330 kb on - strand, within GFF755at 20.656 kb on - strand, within GFF756at 20.656 kb on - strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.677 kb on + strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.678 kb on - strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.681 kb on + strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.682 kb on - strand, within GFF756at 20.778 kb on + strandat 20.778 kb on + strandat 20.778 kb on + strandat 20.778 kb on + strandat 20.779 kb on - strandat 20.779 kb on - strandat 20.779 kb on - strandat 20.779 kb on - strandat 20.779 kb on - strandat 20.779 kb on - strandat 20.779 kb on - strandat 21.059 kb on + strand, within GFF757at 21.059 kb on + strand, within GFF757at 21.059 kb on + strand, within GFF757at 21.059 kb on + strand, within GFF757at 21.059 kb on + strand, within GFF757at 21.060 kb on - strand, within GFF757at 21.060 kb on - strand, within GFF757at 21.236 kb on + strand, within GFF757at 21.550 kb on + strandat 21.550 kb on + strandat 21.550 kb on + strandat 21.551 kb on - strandat 22.405 kb on + strandat 22.405 kb on + strandat 22.406 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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19,835 + GFF755 0.61 +1.5
19,835 + GFF755 0.61 -1.2
19,835 + GFF755 0.61 -0.7
19,835 + GFF755 0.61 -1.0
19,836 - GFF755 0.61 +0.7
19,836 - GFF755 0.61 -0.8
19,836 - GFF755 0.61 -0.0
20,224 + GFF755 0.82 +0.7
20,224 + GFF755 0.82 +0.9
20,225 - GFF755 0.82 +1.1
20,225 - GFF755 0.82 +0.3
20,302 + GFF755 0.86 -0.0
20,329 + GFF755 0.87 -0.1
20,329 + GFF755 0.87 +0.0
20,329 + GFF755 0.87 -0.5
20,330 - GFF755 0.87 -0.5
20,330 - GFF755 0.87 +0.1
20,330 - GFF755 0.87 -0.8
20,656 - GFF756 0.62 +1.1
20,656 - GFF756 0.62 -0.1
20,677 + GFF756 0.76 +0.2
20,677 + GFF756 0.76 -1.1
20,677 + GFF756 0.76 -0.5
20,677 + GFF756 0.76 -1.2
20,677 + GFF756 0.76 -1.1
20,677 + GFF756 0.76 -0.7
20,677 + GFF756 0.76 +0.3
20,677 + GFF756 0.76 +0.3
20,677 + GFF756 0.76 +0.8
20,677 + GFF756 0.76 +0.7
20,677 + GFF756 0.76 -1.3
20,677 + GFF756 0.76 -0.1
20,677 + GFF756 0.76 +0.2
20,677 + GFF756 0.76 -0.1
20,677 + GFF756 0.76 -0.1
20,678 - GFF756 0.77 +0.1
20,678 - GFF756 0.77 +0.1
20,678 - GFF756 0.77 +0.0
20,678 - GFF756 0.77 -0.3
20,678 - GFF756 0.77 -0.9
20,678 - GFF756 0.77 -1.3
20,678 - GFF756 0.77 -0.2
20,678 - GFF756 0.77 -0.9
20,678 - GFF756 0.77 -0.3
20,678 - GFF756 0.77 -0.4
20,678 - GFF756 0.77 +0.5
20,681 + GFF756 0.79 -0.0
20,681 + GFF756 0.79 -0.3
20,681 + GFF756 0.79 +1.5
20,681 + GFF756 0.79 -0.7
20,681 + GFF756 0.79 -0.5
20,681 + GFF756 0.79 -0.9
20,681 + GFF756 0.79 -0.3
20,681 + GFF756 0.79 -0.2
20,681 + GFF756 0.79 -0.5
20,682 - GFF756 0.80 -0.3
20,682 - GFF756 0.80 +0.5
20,682 - GFF756 0.80 -1.9
20,682 - GFF756 0.80 +1.2
20,682 - GFF756 0.80 -0.3
20,682 - GFF756 0.80 -1.1
20,682 - GFF756 0.80 -1.0
20,682 - GFF756 0.80 -0.2
20,682 - GFF756 0.80 -0.0
20,682 - GFF756 0.80 +0.9
20,682 - GFF756 0.80 -0.1
20,682 - GFF756 0.80 +0.4
20,778 + -0.6
20,778 + +0.2
20,778 + -2.9
20,778 + +0.2
20,779 - -1.7
20,779 - -0.5
20,779 - -0.1
20,779 - -0.2
20,779 - -0.2
20,779 - +0.9
20,779 - -0.5
21,059 + GFF757 0.42 -1.0
21,059 + GFF757 0.42 -0.8
21,059 + GFF757 0.42 -0.1
21,059 + GFF757 0.42 -0.9
21,059 + GFF757 0.42 -0.4
21,060 - GFF757 0.43 -0.8
21,060 - GFF757 0.43 +1.3
21,236 + GFF757 0.64 -0.0
21,550 + -0.7
21,550 + +1.2
21,550 + +0.9
21,551 - -0.0
22,405 + +0.7
22,405 + -0.5
22,406 - -0.4

Or see this region's nucleotide sequence