Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7323 and GFF7324 are separated by 193 nucleotides GFF7324 and GFF7325 are separated by 5 nucleotides
GFF7323 - Transcriptional response regulatory protein GlrR, at 18,256 to 19,644
GFF7323
GFF7324 - hypothetical protein, at 19,838 to 20,041
GFF7324
GFF7325 - Glucans biosynthesis glucosyltransferase H, at 20,047 to 22,053
GFF7325
Position (kb) 19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.153 kb on + strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.154 kb on - strand, within GFF7323 at 19.157 kb on - strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.251 kb on + strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.252 kb on - strand, within GFF7323 at 19.258 kb on + strand, within GFF7323 at 19.258 kb on + strand, within GFF7323 at 19.258 kb on + strand, within GFF7323 at 19.259 kb on - strand, within GFF7323 at 19.259 kb on - strand, within GFF7323 at 19.259 kb on - strand, within GFF7323 at 19.259 kb on - strand, within GFF7323 at 19.444 kb on + strand, within GFF7323 at 19.444 kb on + strand, within GFF7323 at 19.444 kb on + strand, within GFF7323 at 19.444 kb on + strand, within GFF7323 at 19.444 kb on + strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.445 kb on - strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.477 kb on + strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.478 kb on - strand, within GFF7323 at 19.558 kb on + strand at 19.558 kb on + strand at 19.559 kb on - strand at 19.654 kb on + strand at 19.654 kb on + strand at 19.655 kb on - strand at 19.655 kb on - strand at 19.655 kb on - strand at 19.655 kb on - strand at 19.655 kb on - strand at 19.655 kb on - strand at 19.670 kb on - strand at 19.757 kb on + strand at 19.758 kb on - strand at 19.831 kb on + strand at 19.831 kb on + strand at 19.832 kb on - strand at 19.832 kb on - strand at 19.832 kb on - strand at 19.934 kb on + strand, within GFF7324 at 19.934 kb on + strand, within GFF7324 at 19.934 kb on + strand, within GFF7324 at 19.935 kb on - strand, within GFF7324 at 19.935 kb on - strand, within GFF7324 at 19.935 kb on - strand, within GFF7324 at 20.039 kb on + strand at 20.039 kb on + strand at 20.039 kb on + strand at 20.039 kb on + strand at 20.040 kb on - strand at 20.040 kb on - strand at 20.040 kb on - strand at 20.040 kb on - strand at 20.383 kb on + strand, within GFF7325 at 20.383 kb on + strand, within GFF7325 at 20.383 kb on + strand, within GFF7325 at 20.383 kb on + strand, within GFF7325 at 20.384 kb on - strand, within GFF7325 at 20.384 kb on - strand, within GFF7325 at 20.422 kb on + strand, within GFF7325 at 20.422 kb on + strand, within GFF7325 at 20.422 kb on + strand, within GFF7325 at 20.422 kb on + strand, within GFF7325 at 20.423 kb on - strand, within GFF7325 at 20.423 kb on - strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.479 kb on + strand, within GFF7325 at 20.480 kb on - strand, within GFF7325 at 20.480 kb on - strand, within GFF7325 at 20.480 kb on - strand, within GFF7325 at 20.480 kb on - strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.686 kb on + strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.687 kb on - strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.779 kb on + strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.780 kb on - strand, within GFF7325 at 20.782 kb on + strand, within GFF7325 at 20.782 kb on + strand, within GFF7325 at 20.783 kb on - strand, within GFF7325 at 20.783 kb on - strand, within GFF7325 at 20.857 kb on + strand, within GFF7325 at 20.857 kb on + strand, within GFF7325 at 20.857 kb on + strand, within GFF7325 at 20.858 kb on - strand, within GFF7325 at 20.862 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.863 kb on + strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325 at 20.864 kb on - strand, within GFF7325
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 19,153 + GFF7323 0.65 +0.3 19,153 + GFF7323 0.65 -1.5 19,153 + GFF7323 0.65 -0.8 19,153 + GFF7323 0.65 -1.8 19,153 + GFF7323 0.65 +3.1 19,153 + GFF7323 0.65 +0.1 19,153 + GFF7323 0.65 -0.3 19,153 + GFF7323 0.65 -0.0 19,153 + GFF7323 0.65 -1.3 19,153 + GFF7323 0.65 +0.6 19,153 + GFF7323 0.65 -0.1 19,153 + GFF7323 0.65 -1.7 19,153 + GFF7323 0.65 +0.3 19,154 - GFF7323 0.65 +0.7 19,154 - GFF7323 0.65 -1.4 19,154 - GFF7323 0.65 -0.7 19,154 - GFF7323 0.65 +2.2 19,154 - GFF7323 0.65 +0.0 19,154 - GFF7323 0.65 -0.3 19,154 - GFF7323 0.65 +0.5 19,154 - GFF7323 0.65 -0.2 19,154 - GFF7323 0.65 +0.7 19,154 - GFF7323 0.65 -1.1 19,154 - GFF7323 0.65 -1.3 19,154 - GFF7323 0.65 +0.7 19,154 - GFF7323 0.65 -0.8 19,154 - GFF7323 0.65 +0.2 19,154 - GFF7323 0.65 +1.0 19,154 - GFF7323 0.65 -1.8 19,154 - GFF7323 0.65 +0.3 19,154 - GFF7323 0.65 -0.1 19,154 - GFF7323 0.65 +2.5 19,154 - GFF7323 0.65 -0.1 19,157 - GFF7323 0.65 -2.8 19,251 + GFF7323 0.72 -0.7 19,251 + GFF7323 0.72 -1.0 19,251 + GFF7323 0.72 -0.3 19,251 + GFF7323 0.72 +0.4 19,251 + GFF7323 0.72 -0.5 19,251 + GFF7323 0.72 -0.4 19,251 + GFF7323 0.72 +0.0 19,252 - GFF7323 0.72 +0.1 19,252 - GFF7323 0.72 +0.7 19,252 - GFF7323 0.72 -0.5 19,252 - GFF7323 0.72 -0.8 19,252 - GFF7323 0.72 -0.2 19,252 - GFF7323 0.72 +0.5 19,258 + GFF7323 0.72 +1.1 19,258 + GFF7323 0.72 +0.3 19,258 + GFF7323 0.72 +0.9 19,259 - GFF7323 0.72 +0.7 19,259 - GFF7323 0.72 -0.8 19,259 - GFF7323 0.72 +0.4 19,259 - GFF7323 0.72 -0.5 19,444 + GFF7323 0.86 -0.1 19,444 + GFF7323 0.86 -0.4 19,444 + GFF7323 0.86 -0.3 19,444 + GFF7323 0.86 -0.2 19,444 + GFF7323 0.86 +1.5 19,445 - GFF7323 0.86 -0.9 19,445 - GFF7323 0.86 -0.3 19,445 - GFF7323 0.86 -0.7 19,445 - GFF7323 0.86 -1.1 19,445 - GFF7323 0.86 -0.8 19,445 - GFF7323 0.86 -0.2 19,445 - GFF7323 0.86 +0.2 19,445 - GFF7323 0.86 -0.5 19,477 + GFF7323 0.88 -0.3 19,477 + GFF7323 0.88 +0.1 19,477 + GFF7323 0.88 -0.3 19,477 + GFF7323 0.88 -2.0 19,477 + GFF7323 0.88 +0.4 19,477 + GFF7323 0.88 +0.3 19,477 + GFF7323 0.88 -0.1 19,478 - GFF7323 0.88 -0.1 19,478 - GFF7323 0.88 -0.2 19,478 - GFF7323 0.88 -0.4 19,478 - GFF7323 0.88 -0.5 19,478 - GFF7323 0.88 +0.6 19,478 - GFF7323 0.88 -0.8 19,558 + -0.6 19,558 + +0.6 19,559 - +0.3 19,654 + +0.7 19,654 + +0.3 19,655 - -1.1 19,655 - -0.2 19,655 - -1.2 19,655 - -0.7 19,655 - -0.4 19,655 - +0.3 19,670 - +1.0 19,757 + -1.0 19,758 - +0.0 19,831 + +0.2 19,831 + +0.9 19,832 - +0.4 19,832 - -1.1 19,832 - -0.4 19,934 + GFF7324 0.47 -0.1 19,934 + GFF7324 0.47 -0.3 19,934 + GFF7324 0.47 -0.7 19,935 - GFF7324 0.48 -0.7 19,935 - GFF7324 0.48 -1.3 19,935 - GFF7324 0.48 -0.4 20,039 + -0.8 20,039 + -2.5 20,039 + -0.3 20,039 + -0.8 20,040 - +0.3 20,040 - -0.2 20,040 - +0.0 20,040 - +0.2 20,383 + GFF7325 0.17 +0.2 20,383 + GFF7325 0.17 +0.2 20,383 + GFF7325 0.17 +0.2 20,383 + GFF7325 0.17 +0.9 20,384 - GFF7325 0.17 -1.5 20,384 - GFF7325 0.17 -1.5 20,422 + GFF7325 0.19 -2.0 20,422 + GFF7325 0.19 -0.4 20,422 + GFF7325 0.19 +0.5 20,422 + GFF7325 0.19 -0.3 20,423 - GFF7325 0.19 -0.4 20,423 - GFF7325 0.19 +0.0 20,479 + GFF7325 0.22 -2.2 20,479 + GFF7325 0.22 -0.1 20,479 + GFF7325 0.22 +0.1 20,479 + GFF7325 0.22 -1.0 20,479 + GFF7325 0.22 +0.1 20,479 + GFF7325 0.22 +0.4 20,479 + GFF7325 0.22 -0.5 20,479 + GFF7325 0.22 +0.2 20,479 + GFF7325 0.22 -1.0 20,480 - GFF7325 0.22 -0.4 20,480 - GFF7325 0.22 +0.2 20,480 - GFF7325 0.22 -0.6 20,480 - GFF7325 0.22 +0.3 20,686 + GFF7325 0.32 -2.0 20,686 + GFF7325 0.32 -1.8 20,686 + GFF7325 0.32 +0.1 20,686 + GFF7325 0.32 +2.7 20,686 + GFF7325 0.32 +0.2 20,686 + GFF7325 0.32 -0.6 20,686 + GFF7325 0.32 -1.4 20,687 - GFF7325 0.32 -0.7 20,687 - GFF7325 0.32 -0.3 20,687 - GFF7325 0.32 -0.4 20,687 - GFF7325 0.32 -0.5 20,687 - GFF7325 0.32 -0.9 20,687 - GFF7325 0.32 -0.3 20,687 - GFF7325 0.32 -0.6 20,687 - GFF7325 0.32 -0.8 20,687 - GFF7325 0.32 +1.5 20,687 - GFF7325 0.32 -0.2 20,687 - GFF7325 0.32 -1.6 20,687 - GFF7325 0.32 +0.2 20,687 - GFF7325 0.32 -0.6 20,687 - GFF7325 0.32 -0.1 20,779 + GFF7325 0.36 -0.9 20,779 + GFF7325 0.36 -0.8 20,779 + GFF7325 0.36 -0.3 20,779 + GFF7325 0.36 +0.6 20,779 + GFF7325 0.36 +0.2 20,779 + GFF7325 0.36 +0.9 20,779 + GFF7325 0.36 -3.0 20,779 + GFF7325 0.36 -0.8 20,779 + GFF7325 0.36 -0.4 20,779 + GFF7325 0.36 -1.0 20,779 + GFF7325 0.36 -0.1 20,779 + GFF7325 0.36 +0.6 20,779 + GFF7325 0.36 -0.4 20,779 + GFF7325 0.36 +0.2 20,779 + GFF7325 0.36 +1.0 20,779 + GFF7325 0.36 -0.3 20,779 + GFF7325 0.36 -0.6 20,779 + GFF7325 0.36 +0.9 20,779 + GFF7325 0.36 +2.3 20,779 + GFF7325 0.36 +0.2 20,779 + GFF7325 0.36 -0.8 20,779 + GFF7325 0.36 -1.3 20,780 - GFF7325 0.37 -0.9 20,780 - GFF7325 0.37 -0.9 20,780 - GFF7325 0.37 +1.7 20,780 - GFF7325 0.37 -0.1 20,780 - GFF7325 0.37 -0.4 20,780 - GFF7325 0.37 -1.6 20,780 - GFF7325 0.37 -0.0 20,780 - GFF7325 0.37 -1.3 20,780 - GFF7325 0.37 -2.2 20,780 - GFF7325 0.37 +0.6 20,780 - GFF7325 0.37 +0.6 20,780 - GFF7325 0.37 -0.7 20,780 - GFF7325 0.37 -0.3 20,780 - GFF7325 0.37 -0.2 20,780 - GFF7325 0.37 -0.5 20,780 - GFF7325 0.37 -1.0 20,780 - GFF7325 0.37 -0.6 20,780 - GFF7325 0.37 -0.5 20,780 - GFF7325 0.37 +0.7 20,782 + GFF7325 0.37 -0.8 20,782 + GFF7325 0.37 -1.8 20,783 - GFF7325 0.37 +0.3 20,783 - GFF7325 0.37 -0.3 20,857 + GFF7325 0.40 +0.5 20,857 + GFF7325 0.40 -0.3 20,857 + GFF7325 0.40 +0.1 20,858 - GFF7325 0.40 +1.2 20,862 + GFF7325 0.41 -1.1 20,863 + GFF7325 0.41 -1.7 20,863 + GFF7325 0.41 -0.5 20,863 + GFF7325 0.41 +1.1 20,863 + GFF7325 0.41 +1.7 20,863 + GFF7325 0.41 -0.1 20,863 + GFF7325 0.41 -0.6 20,863 + GFF7325 0.41 -0.3 20,863 + GFF7325 0.41 -0.2 20,863 + GFF7325 0.41 -0.9 20,863 + GFF7325 0.41 +0.3 20,863 + GFF7325 0.41 -0.3 20,864 - GFF7325 0.41 +0.6 20,864 - GFF7325 0.41 +0.2 20,864 - GFF7325 0.41 +0.0 20,864 - GFF7325 0.41 -0.3 20,864 - GFF7325 0.41 +0.5 20,864 - GFF7325 0.41 +0.2 20,864 - GFF7325 0.41 -0.8 20,864 - GFF7325 0.41 -0.3 20,864 - GFF7325 0.41 +1.0 20,864 - GFF7325 0.41 +0.3 20,864 - GFF7325 0.41 -0.1 20,864 - GFF7325 0.41 -0.6 20,864 - GFF7325 0.41 -2.2 20,864 - GFF7325 0.41 -0.6 20,864 - GFF7325 0.41 +0.7
Or see this region's nucleotide sequence