Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7283 and GFF7284 are separated by 51 nucleotides GFF7284 and GFF7285 are separated by 4 nucleotides
GFF7283 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 226 to 1,905
GFF7283
GFF7284 - Dipeptide ABC transporter, permease protein DppB (TC 3.A.1.5.2), at 1,957 to 2,961
GFF7284
GFF7285 - Dipeptide ABC transporter, permease protein DppC (TC 3.A.1.5.2), at 2,966 to 3,871
GFF7285
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.015 kb on + strand, within GFF7283 at 1.015 kb on + strand, within GFF7283 at 1.015 kb on + strand, within GFF7283 at 1.016 kb on - strand, within GFF7283 at 1.016 kb on - strand, within GFF7283 at 1.307 kb on - strand, within GFF7283 at 1.363 kb on + strand, within GFF7283 at 1.636 kb on + strand, within GFF7283 at 1.637 kb on - strand, within GFF7283 at 1.787 kb on - strand at 1.787 kb on - strand at 1.803 kb on - strand at 1.803 kb on - strand at 1.810 kb on + strand at 1.810 kb on + strand at 1.810 kb on + strand at 1.811 kb on - strand at 1.811 kb on - strand at 1.811 kb on - strand at 1.811 kb on - strand at 1.829 kb on + strand at 1.903 kb on + strand at 1.904 kb on - strand at 1.920 kb on + strand at 1.944 kb on + strand at 1.944 kb on + strand at 1.944 kb on + strand at 1.944 kb on + strand at 1.945 kb on - strand at 2.017 kb on - strand at 2.122 kb on + strand, within GFF7284 at 2.152 kb on + strand, within GFF7284 at 2.161 kb on + strand, within GFF7284 at 2.161 kb on + strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.162 kb on - strand, within GFF7284 at 2.473 kb on + strand, within GFF7284 at 2.474 kb on - strand, within GFF7284 at 2.474 kb on - strand, within GFF7284 at 2.474 kb on - strand, within GFF7284 at 2.474 kb on - strand, within GFF7284 at 2.474 kb on - strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.476 kb on + strand, within GFF7284 at 2.477 kb on - strand, within GFF7284 at 2.477 kb on - strand, within GFF7284 at 2.477 kb on - strand, within GFF7284 at 2.564 kb on + strand, within GFF7284 at 2.564 kb on + strand, within GFF7284 at 2.565 kb on - strand, within GFF7284 at 2.653 kb on + strand, within GFF7284 at 2.653 kb on + strand, within GFF7284 at 2.653 kb on + strand, within GFF7284 at 2.653 kb on + strand, within GFF7284 at 2.654 kb on - strand, within GFF7284 at 2.654 kb on - strand, within GFF7284 at 2.654 kb on - strand, within GFF7284 at 2.854 kb on + strand, within GFF7284 at 2.929 kb on + strand at 2.929 kb on + strand at 2.929 kb on + strand at 2.929 kb on + strand at 2.930 kb on - strand at 3.204 kb on - strand, within GFF7285 at 3.254 kb on + strand, within GFF7285 at 3.254 kb on + strand, within GFF7285 at 3.254 kb on + strand, within GFF7285 at 3.255 kb on - strand, within GFF7285 at 3.255 kb on - strand, within GFF7285 at 3.255 kb on - strand, within GFF7285 at 3.255 kb on - strand, within GFF7285 at 3.255 kb on - strand, within GFF7285 at 3.494 kb on + strand, within GFF7285 at 3.494 kb on + strand, within GFF7285 at 3.494 kb on + strand, within GFF7285 at 3.494 kb on + strand, within GFF7285 at 3.495 kb on - strand, within GFF7285 at 3.495 kb on - strand, within GFF7285 at 3.542 kb on + strand, within GFF7285 at 3.542 kb on + strand, within GFF7285 at 3.542 kb on + strand, within GFF7285 at 3.542 kb on + strand, within GFF7285 at 3.543 kb on - strand, within GFF7285 at 3.543 kb on - strand, within GFF7285 at 3.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 1,015 + GFF7283 0.47 -0.6 1,015 + GFF7283 0.47 -0.0 1,015 + GFF7283 0.47 +0.2 1,016 - GFF7283 0.47 +1.2 1,016 - GFF7283 0.47 +1.5 1,307 - GFF7283 0.64 -0.7 1,363 + GFF7283 0.68 -0.2 1,636 + GFF7283 0.84 -1.5 1,637 - GFF7283 0.84 +0.9 1,787 - +1.2 1,787 - +0.5 1,803 - -0.5 1,803 - -1.3 1,810 + +0.6 1,810 + -0.5 1,810 + -0.4 1,811 - -2.2 1,811 - -1.8 1,811 - -0.3 1,811 - -0.3 1,829 + -1.5 1,903 + -0.2 1,904 - -0.2 1,920 + +0.4 1,944 + -1.4 1,944 + -0.9 1,944 + -0.5 1,944 + +0.1 1,945 - -0.3 2,017 - -0.4 2,122 + GFF7284 0.16 -1.6 2,152 + GFF7284 0.19 -0.2 2,161 + GFF7284 0.20 +0.0 2,161 + GFF7284 0.20 -1.0 2,162 - GFF7284 0.20 +1.0 2,162 - GFF7284 0.20 +0.2 2,162 - GFF7284 0.20 +0.9 2,162 - GFF7284 0.20 -0.8 2,162 - GFF7284 0.20 -0.5 2,162 - GFF7284 0.20 -1.2 2,473 + GFF7284 0.51 -0.3 2,474 - GFF7284 0.51 -0.9 2,474 - GFF7284 0.51 -0.2 2,474 - GFF7284 0.51 -0.5 2,474 - GFF7284 0.51 -0.8 2,474 - GFF7284 0.51 -0.2 2,476 + GFF7284 0.52 +0.2 2,476 + GFF7284 0.52 +0.3 2,476 + GFF7284 0.52 -0.1 2,476 + GFF7284 0.52 +0.5 2,476 + GFF7284 0.52 -0.3 2,476 + GFF7284 0.52 -0.8 2,476 + GFF7284 0.52 -0.2 2,476 + GFF7284 0.52 -0.6 2,476 + GFF7284 0.52 +1.0 2,476 + GFF7284 0.52 -0.3 2,477 - GFF7284 0.52 +0.1 2,477 - GFF7284 0.52 -0.8 2,477 - GFF7284 0.52 +2.0 2,564 + GFF7284 0.60 -1.5 2,564 + GFF7284 0.60 -0.0 2,565 - GFF7284 0.60 +0.0 2,653 + GFF7284 0.69 +1.0 2,653 + GFF7284 0.69 +0.9 2,653 + GFF7284 0.69 +0.6 2,653 + GFF7284 0.69 -0.1 2,654 - GFF7284 0.69 -0.2 2,654 - GFF7284 0.69 +0.2 2,654 - GFF7284 0.69 -0.5 2,854 + GFF7284 0.89 -0.5 2,929 + -0.1 2,929 + -0.2 2,929 + +1.1 2,929 + -0.3 2,930 - -1.7 3,204 - GFF7285 0.26 -0.3 3,254 + GFF7285 0.32 +0.2 3,254 + GFF7285 0.32 -0.8 3,254 + GFF7285 0.32 -0.5 3,255 - GFF7285 0.32 +0.2 3,255 - GFF7285 0.32 -0.7 3,255 - GFF7285 0.32 +1.8 3,255 - GFF7285 0.32 +0.2 3,255 - GFF7285 0.32 +0.7 3,494 + GFF7285 0.58 -1.2 3,494 + GFF7285 0.58 +0.1 3,494 + GFF7285 0.58 -0.7 3,494 + GFF7285 0.58 +0.8 3,495 - GFF7285 0.58 -0.5 3,495 - GFF7285 0.58 +0.6 3,542 + GFF7285 0.64 -0.3 3,542 + GFF7285 0.64 -0.8 3,542 + GFF7285 0.64 -1.0 3,542 + GFF7285 0.64 +0.4 3,543 - GFF7285 0.64 -0.5 3,543 - GFF7285 0.64 +0.7 3,870 - -0.3
Or see this region's nucleotide sequence