Strain Fitness in Variovorax sp. SCN45 around GFF7169

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7167 and GFF7168 are separated by 12 nucleotidesGFF7168 and GFF7169 are separated by 55 nucleotidesGFF7169 and GFF7170 are separated by 88 nucleotides GFF7167 - Pirin, at 31,412 to 32,467 GFF7167 GFF7168 - hypothetical protein, at 32,480 to 32,770 GFF7168 GFF7169 - BUG/TctC family periplasmic protein, at 32,826 to 33,860 GFF7169 GFF7170 - D-malate dehydrogenase [decarboxylating] (EC 1.1.1.83), at 33,949 to 35,040 GFF7170 Position (kb) 32 33 34Strain fitness (log2 ratio) -2 -1 0 1at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.135 kb on + strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.136 kb on - strand, within GFF7167at 32.297 kb on + strand, within GFF7167at 32.297 kb on + strand, within GFF7167at 32.298 kb on - strand, within GFF7167at 32.298 kb on - strand, within GFF7167at 32.359 kb on + strand, within GFF7167at 32.359 kb on + strand, within GFF7167at 32.359 kb on + strand, within GFF7167at 32.359 kb on + strand, within GFF7167at 32.360 kb on - strand, within GFF7167at 32.360 kb on - strand, within GFF7167at 32.360 kb on - strand, within GFF7167at 32.360 kb on - strand, within GFF7167at 32.360 kb on - strand, within GFF7167at 32.682 kb on + strand, within GFF7168at 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.748 kb on + strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.749 kb on - strandat 32.809 kb on + strandat 32.809 kb on + strandat 32.809 kb on + strandat 32.810 kb on - strandat 32.810 kb on - strandat 32.810 kb on - strandat 33.247 kb on + strand, within GFF7169at 33.247 kb on + strand, within GFF7169at 33.247 kb on + strand, within GFF7169at 33.247 kb on + strand, within GFF7169at 33.247 kb on + strand, within GFF7169at 33.247 kb on + strand, within GFF7169at 33.248 kb on - strand, within GFF7169at 33.248 kb on - strand, within GFF7169at 33.248 kb on - strand, within GFF7169at 33.335 kb on - strand, within GFF7169at 33.335 kb on - strand, within GFF7169at 33.475 kb on + strand, within GFF7169at 33.490 kb on + strand, within GFF7169at 33.490 kb on + strand, within GFF7169at 33.490 kb on + strand, within GFF7169at 33.490 kb on + strand, within GFF7169at 33.539 kb on - strand, within GFF7169at 33.539 kb on - strand, within GFF7169at 33.565 kb on + strand, within GFF7169at 33.565 kb on + strand, within GFF7169at 33.565 kb on + strand, within GFF7169at 33.566 kb on - strand, within GFF7169at 33.566 kb on - strand, within GFF7169at 33.566 kb on - strand, within GFF7169at 33.881 kb on + strandat 33.881 kb on + strandat 33.881 kb on + strandat 33.881 kb on + strandat 33.881 kb on + strandat 33.882 kb on - strandat 33.882 kb on - strandat 33.909 kb on + strandat 34.625 kb on + strand, within GFF7170at 34.625 kb on + strand, within GFF7170at 34.626 kb on - strand, within GFF7170at 34.737 kb on - strand, within GFF7170at 34.848 kb on + strand, within GFF7170

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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32,135 + GFF7167 0.68 -0.2
32,135 + GFF7167 0.68 +0.4
32,135 + GFF7167 0.68 +0.2
32,135 + GFF7167 0.68 +0.4
32,135 + GFF7167 0.68 -0.0
32,135 + GFF7167 0.68 -0.9
32,135 + GFF7167 0.68 +1.7
32,135 + GFF7167 0.68 -0.6
32,136 - GFF7167 0.69 -1.9
32,136 - GFF7167 0.69 -0.0
32,136 - GFF7167 0.69 +0.3
32,136 - GFF7167 0.69 +0.2
32,136 - GFF7167 0.69 -0.1
32,136 - GFF7167 0.69 -0.6
32,136 - GFF7167 0.69 -0.2
32,136 - GFF7167 0.69 -2.1
32,136 - GFF7167 0.69 -0.4
32,136 - GFF7167 0.69 -1.0
32,297 + GFF7167 0.84 -0.2
32,297 + GFF7167 0.84 -0.5
32,298 - GFF7167 0.84 -0.4
32,298 - GFF7167 0.84 -0.3
32,359 + GFF7167 0.90 -0.9
32,359 + GFF7167 0.90 -0.3
32,359 + GFF7167 0.90 +0.4
32,359 + GFF7167 0.90 +0.6
32,360 - GFF7167 0.90 -0.8
32,360 - GFF7167 0.90 -1.5
32,360 - GFF7167 0.90 -0.3
32,360 - GFF7167 0.90 +0.1
32,360 - GFF7167 0.90 -0.3
32,682 + GFF7168 0.69 +1.6
32,748 + +0.6
32,748 + +0.4
32,748 + -1.5
32,748 + -0.8
32,748 + -0.1
32,748 + +0.9
32,748 + -0.4
32,748 + +0.2
32,748 + -1.1
32,748 + -0.6
32,749 - +0.0
32,749 - -0.5
32,749 - -1.0
32,749 - -0.1
32,749 - +0.1
32,749 - -0.7
32,749 - -0.7
32,749 - -0.3
32,749 - +0.1
32,749 - +0.4
32,749 - -0.6
32,809 + -0.2
32,809 + -0.2
32,809 + -1.0
32,810 - -2.2
32,810 - +0.6
32,810 - +0.1
33,247 + GFF7169 0.41 +0.3
33,247 + GFF7169 0.41 +0.7
33,247 + GFF7169 0.41 +0.1
33,247 + GFF7169 0.41 +0.8
33,247 + GFF7169 0.41 -0.1
33,247 + GFF7169 0.41 +0.1
33,248 - GFF7169 0.41 -0.7
33,248 - GFF7169 0.41 -0.3
33,248 - GFF7169 0.41 +1.0
33,335 - GFF7169 0.49 -0.3
33,335 - GFF7169 0.49 -0.3
33,475 + GFF7169 0.63 +0.6
33,490 + GFF7169 0.64 +1.4
33,490 + GFF7169 0.64 +0.3
33,490 + GFF7169 0.64 -0.4
33,490 + GFF7169 0.64 +0.1
33,539 - GFF7169 0.69 -0.4
33,539 - GFF7169 0.69 +0.0
33,565 + GFF7169 0.71 -0.0
33,565 + GFF7169 0.71 +1.5
33,565 + GFF7169 0.71 -0.1
33,566 - GFF7169 0.71 -0.7
33,566 - GFF7169 0.71 -1.6
33,566 - GFF7169 0.71 -0.1
33,881 + -0.6
33,881 + -0.5
33,881 + -1.2
33,881 + +0.4
33,881 + -1.1
33,882 - -0.6
33,882 - -0.6
33,909 + -0.8
34,625 + GFF7170 0.62 -0.9
34,625 + GFF7170 0.62 +0.2
34,626 - GFF7170 0.62 +0.5
34,737 - GFF7170 0.72 +0.8
34,848 + GFF7170 0.82 +0.6

Or see this region's nucleotide sequence