Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7097 and GFF7098 are separated by 48 nucleotides GFF7098 and GFF7099 are separated by 47 nucleotides GFF7099 and GFF7100 are separated by 1 nucleotides GFF7100 and GFF7101 are separated by 2 nucleotides
GFF7097 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 15,886 to 17,340
GFF7097
GFF7098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 17,389 to 18,222
GFF7098
GFF7099 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 18,270 to 18,701
GFF7099
GFF7100 - similar to protocatechuate 4,5-dioxygenase beta chain, at 18,703 to 19,521
GFF7100
GFF7101 - hypothetical protein, at 19,524 to 19,850
GFF7101
Position (kb)
18
19 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 17.366 kb on + strand at 17.366 kb on + strand at 17.366 kb on + strand at 17.644 kb on + strand, within GFF7098 at 17.644 kb on + strand, within GFF7098 at 17.644 kb on + strand, within GFF7098 at 17.644 kb on + strand, within GFF7098 at 17.644 kb on + strand, within GFF7098 at 17.645 kb on - strand, within GFF7098 at 17.645 kb on - strand, within GFF7098 at 17.645 kb on - strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.717 kb on + strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.718 kb on - strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.795 kb on + strand, within GFF7098 at 17.796 kb on - strand, within GFF7098 at 17.796 kb on - strand, within GFF7098 at 17.796 kb on - strand, within GFF7098 at 17.796 kb on - strand, within GFF7098 at 17.796 kb on - strand, within GFF7098 at 17.984 kb on + strand, within GFF7098 at 17.985 kb on - strand, within GFF7098 at 18.105 kb on - strand, within GFF7098 at 18.313 kb on + strand at 18.313 kb on + strand at 18.440 kb on + strand, within GFF7099 at 18.441 kb on - strand, within GFF7099 at 18.441 kb on - strand, within GFF7099 at 18.469 kb on + strand, within GFF7099 at 18.469 kb on + strand, within GFF7099 at 18.469 kb on + strand, within GFF7099 at 18.469 kb on + strand, within GFF7099 at 18.470 kb on - strand, within GFF7099 at 18.470 kb on - strand, within GFF7099 at 18.470 kb on - strand, within GFF7099 at 18.481 kb on + strand, within GFF7099 at 18.481 kb on + strand, within GFF7099 at 18.482 kb on - strand, within GFF7099 at 18.557 kb on - strand, within GFF7099 at 18.557 kb on - strand, within GFF7099 at 18.679 kb on + strand at 18.679 kb on + strand at 18.866 kb on + strand, within GFF7100 at 18.866 kb on + strand, within GFF7100 at 18.867 kb on - strand, within GFF7100 at 19.212 kb on + strand, within GFF7100 at 19.295 kb on + strand, within GFF7100 at 19.296 kb on - strand, within GFF7100 at 19.296 kb on - strand, within GFF7100 at 19.430 kb on + strand, within GFF7100 at 19.431 kb on - strand, within GFF7100 at 19.431 kb on - strand, within GFF7100 at 19.431 kb on - strand, within GFF7100 at 19.525 kb on + strand at 19.526 kb on - strand at 19.526 kb on - strand at 19.591 kb on + strand, within GFF7101 at 19.592 kb on - strand, within GFF7101 at 19.624 kb on + strand, within GFF7101 at 19.624 kb on + strand, within GFF7101 at 19.624 kb on + strand, within GFF7101 at 19.625 kb on - strand, within GFF7101 at 19.625 kb on - strand, within GFF7101 at 19.625 kb on - strand, within GFF7101 at 19.625 kb on - strand, within GFF7101 at 19.665 kb on + strand, within GFF7101 at 19.666 kb on - strand, within GFF7101 at 19.666 kb on - strand, within GFF7101 at 19.666 kb on - strand, within GFF7101 at 19.693 kb on + strand, within GFF7101 at 19.693 kb on + strand, within GFF7101 at 19.694 kb on - strand, within GFF7101 at 19.694 kb on - strand, within GFF7101 at 19.694 kb on - strand, within GFF7101 at 19.694 kb on - strand, within GFF7101 at 19.694 kb on - strand, within GFF7101 at 19.694 kb on - strand, within GFF7101
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 17,366 + -0.5 17,366 + +0.3 17,366 + -0.1 17,644 + GFF7098 0.31 +0.2 17,644 + GFF7098 0.31 -0.5 17,644 + GFF7098 0.31 -0.1 17,644 + GFF7098 0.31 -0.2 17,644 + GFF7098 0.31 -0.1 17,645 - GFF7098 0.31 -0.1 17,645 - GFF7098 0.31 +0.0 17,645 - GFF7098 0.31 -0.1 17,717 + GFF7098 0.39 -1.7 17,717 + GFF7098 0.39 -0.1 17,717 + GFF7098 0.39 -0.5 17,717 + GFF7098 0.39 +0.2 17,717 + GFF7098 0.39 -0.4 17,717 + GFF7098 0.39 -1.2 17,717 + GFF7098 0.39 -0.2 17,717 + GFF7098 0.39 -0.3 17,717 + GFF7098 0.39 -0.5 17,717 + GFF7098 0.39 -0.2 17,718 - GFF7098 0.39 -2.1 17,718 - GFF7098 0.39 -0.5 17,718 - GFF7098 0.39 +0.6 17,718 - GFF7098 0.39 +0.7 17,718 - GFF7098 0.39 +0.2 17,718 - GFF7098 0.39 -0.1 17,718 - GFF7098 0.39 +0.8 17,718 - GFF7098 0.39 -0.5 17,718 - GFF7098 0.39 +0.1 17,795 + GFF7098 0.49 -0.2 17,795 + GFF7098 0.49 -0.4 17,795 + GFF7098 0.49 +0.9 17,795 + GFF7098 0.49 -0.0 17,795 + GFF7098 0.49 -0.1 17,795 + GFF7098 0.49 -1.3 17,795 + GFF7098 0.49 -1.4 17,796 - GFF7098 0.49 +0.0 17,796 - GFF7098 0.49 +1.2 17,796 - GFF7098 0.49 +0.2 17,796 - GFF7098 0.49 -0.5 17,796 - GFF7098 0.49 -1.2 17,984 + GFF7098 0.71 -2.7 17,985 - GFF7098 0.71 -0.5 18,105 - GFF7098 0.86 -0.7 18,313 + -0.4 18,313 + -0.0 18,440 + GFF7099 0.39 +0.3 18,441 - GFF7099 0.40 -0.5 18,441 - GFF7099 0.40 -1.6 18,469 + GFF7099 0.46 -0.6 18,469 + GFF7099 0.46 -0.6 18,469 + GFF7099 0.46 -0.4 18,469 + GFF7099 0.46 +0.3 18,470 - GFF7099 0.46 -0.1 18,470 - GFF7099 0.46 -0.5 18,470 - GFF7099 0.46 -0.1 18,481 + GFF7099 0.49 -1.4 18,481 + GFF7099 0.49 -0.6 18,482 - GFF7099 0.49 +0.3 18,557 - GFF7099 0.66 -0.9 18,557 - GFF7099 0.66 -0.0 18,679 + -0.4 18,679 + -0.3 18,866 + GFF7100 0.20 -2.2 18,866 + GFF7100 0.20 +0.6 18,867 - GFF7100 0.20 -0.9 19,212 + GFF7100 0.62 -0.4 19,295 + GFF7100 0.72 -0.6 19,296 - GFF7100 0.72 +0.9 19,296 - GFF7100 0.72 +2.5 19,430 + GFF7100 0.89 +0.0 19,431 - GFF7100 0.89 -0.2 19,431 - GFF7100 0.89 +0.9 19,431 - GFF7100 0.89 +1.9 19,525 + -0.5 19,526 - +1.8 19,526 - +0.9 19,591 + GFF7101 0.20 +0.2 19,592 - GFF7101 0.21 +0.0 19,624 + GFF7101 0.31 +0.2 19,624 + GFF7101 0.31 +0.4 19,624 + GFF7101 0.31 -1.7 19,625 - GFF7101 0.31 -0.1 19,625 - GFF7101 0.31 -1.4 19,625 - GFF7101 0.31 +0.3 19,625 - GFF7101 0.31 +0.2 19,665 + GFF7101 0.43 -1.4 19,666 - GFF7101 0.43 -0.4 19,666 - GFF7101 0.43 +1.9 19,666 - GFF7101 0.43 +0.0 19,693 + GFF7101 0.52 -0.7 19,693 + GFF7101 0.52 +0.4 19,694 - GFF7101 0.52 -0.6 19,694 - GFF7101 0.52 -1.2 19,694 - GFF7101 0.52 -0.8 19,694 - GFF7101 0.52 -0.7 19,694 - GFF7101 0.52 +0.1 19,694 - GFF7101 0.52 +0.5
Or see this region's nucleotide sequence