Strain Fitness in Variovorax sp. SCN45 around GFF7099

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7097 and GFF7098 are separated by 48 nucleotidesGFF7098 and GFF7099 are separated by 47 nucleotidesGFF7099 and GFF7100 are separated by 1 nucleotidesGFF7100 and GFF7101 are separated by 2 nucleotides GFF7097 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 15,886 to 17,340 GFF7097 GFF7098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 17,389 to 18,222 GFF7098 GFF7099 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 18,270 to 18,701 GFF7099 GFF7100 - similar to protocatechuate 4,5-dioxygenase beta chain, at 18,703 to 19,521 GFF7100 GFF7101 - hypothetical protein, at 19,524 to 19,850 GFF7101 Position (kb) 18 19Strain fitness (log2 ratio) -2 -1 0 1 2at 17.366 kb on + strandat 17.366 kb on + strandat 17.366 kb on + strandat 17.644 kb on + strand, within GFF7098at 17.644 kb on + strand, within GFF7098at 17.644 kb on + strand, within GFF7098at 17.644 kb on + strand, within GFF7098at 17.644 kb on + strand, within GFF7098at 17.645 kb on - strand, within GFF7098at 17.645 kb on - strand, within GFF7098at 17.645 kb on - strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.717 kb on + strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.718 kb on - strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.795 kb on + strand, within GFF7098at 17.796 kb on - strand, within GFF7098at 17.796 kb on - strand, within GFF7098at 17.796 kb on - strand, within GFF7098at 17.796 kb on - strand, within GFF7098at 17.796 kb on - strand, within GFF7098at 17.984 kb on + strand, within GFF7098at 17.985 kb on - strand, within GFF7098at 18.105 kb on - strand, within GFF7098at 18.313 kb on + strandat 18.313 kb on + strandat 18.440 kb on + strand, within GFF7099at 18.441 kb on - strand, within GFF7099at 18.441 kb on - strand, within GFF7099at 18.469 kb on + strand, within GFF7099at 18.469 kb on + strand, within GFF7099at 18.469 kb on + strand, within GFF7099at 18.469 kb on + strand, within GFF7099at 18.470 kb on - strand, within GFF7099at 18.470 kb on - strand, within GFF7099at 18.470 kb on - strand, within GFF7099at 18.481 kb on + strand, within GFF7099at 18.481 kb on + strand, within GFF7099at 18.482 kb on - strand, within GFF7099at 18.557 kb on - strand, within GFF7099at 18.557 kb on - strand, within GFF7099at 18.679 kb on + strandat 18.679 kb on + strandat 18.866 kb on + strand, within GFF7100at 18.866 kb on + strand, within GFF7100at 18.867 kb on - strand, within GFF7100at 19.212 kb on + strand, within GFF7100at 19.295 kb on + strand, within GFF7100at 19.296 kb on - strand, within GFF7100at 19.296 kb on - strand, within GFF7100at 19.430 kb on + strand, within GFF7100at 19.431 kb on - strand, within GFF7100at 19.431 kb on - strand, within GFF7100at 19.431 kb on - strand, within GFF7100at 19.525 kb on + strandat 19.526 kb on - strandat 19.526 kb on - strandat 19.591 kb on + strand, within GFF7101at 19.592 kb on - strand, within GFF7101at 19.624 kb on + strand, within GFF7101at 19.624 kb on + strand, within GFF7101at 19.624 kb on + strand, within GFF7101at 19.625 kb on - strand, within GFF7101at 19.625 kb on - strand, within GFF7101at 19.625 kb on - strand, within GFF7101at 19.625 kb on - strand, within GFF7101at 19.665 kb on + strand, within GFF7101at 19.666 kb on - strand, within GFF7101at 19.666 kb on - strand, within GFF7101at 19.666 kb on - strand, within GFF7101at 19.693 kb on + strand, within GFF7101at 19.693 kb on + strand, within GFF7101at 19.694 kb on - strand, within GFF7101at 19.694 kb on - strand, within GFF7101at 19.694 kb on - strand, within GFF7101at 19.694 kb on - strand, within GFF7101at 19.694 kb on - strand, within GFF7101at 19.694 kb on - strand, within GFF7101

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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17,366 + -0.5
17,366 + +0.3
17,366 + -0.1
17,644 + GFF7098 0.31 +0.2
17,644 + GFF7098 0.31 -0.5
17,644 + GFF7098 0.31 -0.1
17,644 + GFF7098 0.31 -0.2
17,644 + GFF7098 0.31 -0.1
17,645 - GFF7098 0.31 -0.1
17,645 - GFF7098 0.31 +0.0
17,645 - GFF7098 0.31 -0.1
17,717 + GFF7098 0.39 -1.7
17,717 + GFF7098 0.39 -0.1
17,717 + GFF7098 0.39 -0.5
17,717 + GFF7098 0.39 +0.2
17,717 + GFF7098 0.39 -0.4
17,717 + GFF7098 0.39 -1.2
17,717 + GFF7098 0.39 -0.2
17,717 + GFF7098 0.39 -0.3
17,717 + GFF7098 0.39 -0.5
17,717 + GFF7098 0.39 -0.2
17,718 - GFF7098 0.39 -2.1
17,718 - GFF7098 0.39 -0.5
17,718 - GFF7098 0.39 +0.6
17,718 - GFF7098 0.39 +0.7
17,718 - GFF7098 0.39 +0.2
17,718 - GFF7098 0.39 -0.1
17,718 - GFF7098 0.39 +0.8
17,718 - GFF7098 0.39 -0.5
17,718 - GFF7098 0.39 +0.1
17,795 + GFF7098 0.49 -0.2
17,795 + GFF7098 0.49 -0.4
17,795 + GFF7098 0.49 +0.9
17,795 + GFF7098 0.49 -0.0
17,795 + GFF7098 0.49 -0.1
17,795 + GFF7098 0.49 -1.3
17,795 + GFF7098 0.49 -1.4
17,796 - GFF7098 0.49 +0.0
17,796 - GFF7098 0.49 +1.2
17,796 - GFF7098 0.49 +0.2
17,796 - GFF7098 0.49 -0.5
17,796 - GFF7098 0.49 -1.2
17,984 + GFF7098 0.71 -2.7
17,985 - GFF7098 0.71 -0.5
18,105 - GFF7098 0.86 -0.7
18,313 + -0.4
18,313 + -0.0
18,440 + GFF7099 0.39 +0.3
18,441 - GFF7099 0.40 -0.5
18,441 - GFF7099 0.40 -1.6
18,469 + GFF7099 0.46 -0.6
18,469 + GFF7099 0.46 -0.6
18,469 + GFF7099 0.46 -0.4
18,469 + GFF7099 0.46 +0.3
18,470 - GFF7099 0.46 -0.1
18,470 - GFF7099 0.46 -0.5
18,470 - GFF7099 0.46 -0.1
18,481 + GFF7099 0.49 -1.4
18,481 + GFF7099 0.49 -0.6
18,482 - GFF7099 0.49 +0.3
18,557 - GFF7099 0.66 -0.9
18,557 - GFF7099 0.66 -0.0
18,679 + -0.4
18,679 + -0.3
18,866 + GFF7100 0.20 -2.2
18,866 + GFF7100 0.20 +0.6
18,867 - GFF7100 0.20 -0.9
19,212 + GFF7100 0.62 -0.4
19,295 + GFF7100 0.72 -0.6
19,296 - GFF7100 0.72 +0.9
19,296 - GFF7100 0.72 +2.5
19,430 + GFF7100 0.89 +0.0
19,431 - GFF7100 0.89 -0.2
19,431 - GFF7100 0.89 +0.9
19,431 - GFF7100 0.89 +1.9
19,525 + -0.5
19,526 - +1.8
19,526 - +0.9
19,591 + GFF7101 0.20 +0.2
19,592 - GFF7101 0.21 +0.0
19,624 + GFF7101 0.31 +0.2
19,624 + GFF7101 0.31 +0.4
19,624 + GFF7101 0.31 -1.7
19,625 - GFF7101 0.31 -0.1
19,625 - GFF7101 0.31 -1.4
19,625 - GFF7101 0.31 +0.3
19,625 - GFF7101 0.31 +0.2
19,665 + GFF7101 0.43 -1.4
19,666 - GFF7101 0.43 -0.4
19,666 - GFF7101 0.43 +1.9
19,666 - GFF7101 0.43 +0.0
19,693 + GFF7101 0.52 -0.7
19,693 + GFF7101 0.52 +0.4
19,694 - GFF7101 0.52 -0.6
19,694 - GFF7101 0.52 -1.2
19,694 - GFF7101 0.52 -0.8
19,694 - GFF7101 0.52 -0.7
19,694 - GFF7101 0.52 +0.1
19,694 - GFF7101 0.52 +0.5

Or see this region's nucleotide sequence