Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF686 and GFF687 are separated by 122 nucleotides GFF687 and GFF688 overlap by 4 nucleotides GFF688 and GFF689 overlap by 4 nucleotides
GFF686 - porin, Gram-negative type, at 740,038 to 741,165
GFF686
GFF687 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 741,288 to 742,988
GFF687
GFF688 - Uncharacterized conserved protein, at 742,985 to 743,437
GFF688
GFF689 - protein of unknown function DUF521, at 743,434 to 744,729
GFF689
Position (kb) 741
742
743 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 740.371 kb on - strand, within GFF686 at 740.371 kb on - strand, within GFF686 at 740.390 kb on + strand, within GFF686 at 740.390 kb on + strand, within GFF686 at 740.391 kb on - strand, within GFF686 at 740.391 kb on - strand, within GFF686 at 740.450 kb on + strand, within GFF686 at 740.450 kb on + strand, within GFF686 at 740.450 kb on + strand, within GFF686 at 740.451 kb on - strand, within GFF686 at 740.477 kb on - strand, within GFF686 at 740.612 kb on + strand, within GFF686 at 740.642 kb on + strand, within GFF686 at 740.643 kb on - strand, within GFF686 at 740.643 kb on - strand, within GFF686 at 740.643 kb on - strand, within GFF686 at 740.643 kb on - strand, within GFF686 at 740.753 kb on + strand, within GFF686 at 740.753 kb on + strand, within GFF686 at 740.753 kb on + strand, within GFF686 at 740.753 kb on + strand, within GFF686 at 740.754 kb on - strand, within GFF686 at 740.754 kb on - strand, within GFF686 at 740.754 kb on - strand, within GFF686 at 740.754 kb on - strand, within GFF686 at 740.765 kb on + strand, within GFF686 at 740.765 kb on + strand, within GFF686 at 740.766 kb on - strand, within GFF686 at 740.766 kb on - strand, within GFF686 at 740.766 kb on - strand, within GFF686 at 741.060 kb on - strand at 741.060 kb on - strand at 741.060 kb on - strand at 741.160 kb on - strand at 741.361 kb on + strand at 741.362 kb on - strand at 742.114 kb on + strand, within GFF687 at 742.115 kb on - strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.547 kb on - strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.712 kb on - strand, within GFF687 at 742.712 kb on - strand, within GFF687 at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.949 kb on - strand at 742.949 kb on - strand at 743.441 kb on + strand at 743.442 kb on - strand at 743.442 kb on - strand at 743.531 kb on + strand at 743.531 kb on + strand at 743.531 kb on + strand at 743.771 kb on + strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.796 kb on - strand, within GFF689 at 743.796 kb on - strand, within GFF689 at 743.837 kb on + strand, within GFF689 at 743.838 kb on - strand, within GFF689 at 743.865 kb on - strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 740,371 - GFF686 0.30 +0.1 740,371 - GFF686 0.30 -2.0 740,390 + GFF686 0.31 -0.2 740,390 + GFF686 0.31 -0.2 740,391 - GFF686 0.31 -0.9 740,391 - GFF686 0.31 -2.1 740,450 + GFF686 0.37 +0.3 740,450 + GFF686 0.37 -0.2 740,450 + GFF686 0.37 +1.3 740,451 - GFF686 0.37 -0.6 740,477 - GFF686 0.39 +0.3 740,612 + GFF686 0.51 -0.2 740,642 + GFF686 0.54 -0.1 740,643 - GFF686 0.54 +0.7 740,643 - GFF686 0.54 -0.2 740,643 - GFF686 0.54 -1.0 740,643 - GFF686 0.54 -0.5 740,753 + GFF686 0.63 +0.5 740,753 + GFF686 0.63 +0.1 740,753 + GFF686 0.63 -0.4 740,753 + GFF686 0.63 -1.4 740,754 - GFF686 0.63 -0.3 740,754 - GFF686 0.63 -0.4 740,754 - GFF686 0.63 -1.5 740,754 - GFF686 0.63 -0.1 740,765 + GFF686 0.64 -0.9 740,765 + GFF686 0.64 -0.2 740,766 - GFF686 0.65 +0.6 740,766 - GFF686 0.65 +0.4 740,766 - GFF686 0.65 -0.4 741,060 - -1.5 741,060 - +0.1 741,060 - -0.3 741,160 - -1.2 741,361 + -1.0 741,362 - -1.5 742,114 + GFF687 0.49 -0.2 742,115 - GFF687 0.49 -0.5 742,528 + GFF687 0.73 -1.2 742,528 + GFF687 0.73 +0.8 742,528 + GFF687 0.73 -0.5 742,528 + GFF687 0.73 +2.3 742,529 - GFF687 0.73 -0.3 742,529 - GFF687 0.73 +1.3 742,529 - GFF687 0.73 +0.0 742,529 - GFF687 0.73 -0.7 742,529 - GFF687 0.73 -0.5 742,547 - GFF687 0.74 -1.5 742,711 + GFF687 0.84 -0.7 742,711 + GFF687 0.84 +0.5 742,711 + GFF687 0.84 -0.6 742,711 + GFF687 0.84 +0.1 742,711 + GFF687 0.84 -1.2 742,711 + GFF687 0.84 -1.1 742,712 - GFF687 0.84 -0.4 742,712 - GFF687 0.84 +0.2 742,948 + +0.3 742,948 + +0.2 742,948 + +0.3 742,948 + -1.0 742,948 + +0.5 742,949 - +0.2 742,949 - +0.6 743,441 + -0.0 743,442 - +0.1 743,442 - -0.5 743,531 + -0.6 743,531 + -3.1 743,531 + +1.3 743,771 + GFF689 0.26 +0.4 743,772 - GFF689 0.26 -0.7 743,772 - GFF689 0.26 -0.3 743,772 - GFF689 0.26 -1.0 743,772 - GFF689 0.26 -0.6 743,772 - GFF689 0.26 -0.3 743,772 - GFF689 0.26 -0.4 743,772 - GFF689 0.26 +1.3 743,795 + GFF689 0.28 -0.9 743,795 + GFF689 0.28 +0.3 743,795 + GFF689 0.28 +0.4 743,795 + GFF689 0.28 -1.1 743,795 + GFF689 0.28 +0.4 743,796 - GFF689 0.28 -0.7 743,796 - GFF689 0.28 -0.3 743,837 + GFF689 0.31 +1.9 743,838 - GFF689 0.31 -0.2 743,865 - GFF689 0.33 +1.6 743,945 + GFF689 0.39 -1.2 743,945 + GFF689 0.39 -0.8 743,945 + GFF689 0.39 -0.7 743,945 + GFF689 0.39 -2.3 743,945 + GFF689 0.39 -2.0 743,945 + GFF689 0.39 -0.3 743,945 + GFF689 0.39 -0.0 743,945 + GFF689 0.39 -0.4 743,945 + GFF689 0.39 -0.3 743,945 + GFF689 0.39 +0.7 743,945 + GFF689 0.39 +0.1 743,945 + GFF689 0.39 -0.7 743,946 - GFF689 0.40 +0.9 743,946 - GFF689 0.40 -0.3 743,946 - GFF689 0.40 +0.9 743,946 - GFF689 0.40 -0.5 743,946 - GFF689 0.40 -1.3 743,946 - GFF689 0.40 -0.2
Or see this region's nucleotide sequence