Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6828 and GFF6829 are separated by 27 nucleotides GFF6829 and GFF6830 are separated by 94 nucleotides GFF6830 and GFF6831 are separated by 19 nucleotides GFF6831 and GFF6832 are separated by 63 nucleotides
GFF6828 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 13,526 to 14,164
GFF6828
GFF6829 - Glutathione S-transferase (EC 2.5.1.18), at 14,192 to 14,773
GFF6829
GFF6830 - penicillin amidase family protein, at 14,868 to 17,288
GFF6830
GFF6831 - hypothetical protein, at 17,308 to 17,958
GFF6831
GFF6832 - DnaJ-class molecular chaperone CbpA, at 18,022 to 18,984
GFF6832
Position (kb) 14
15
16
17
18 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 13.880 kb on + strand, within GFF6828 at 13.880 kb on + strand, within GFF6828 at 13.881 kb on - strand, within GFF6828 at 13.916 kb on + strand, within GFF6828 at 13.916 kb on + strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.917 kb on - strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.959 kb on + strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.960 kb on - strand, within GFF6828 at 13.964 kb on + strand, within GFF6828 at 13.964 kb on + strand, within GFF6828 at 14.207 kb on + strand at 14.207 kb on + strand at 14.207 kb on + strand at 14.207 kb on + strand at 14.208 kb on - strand at 14.208 kb on - strand at 14.271 kb on - strand, within GFF6829 at 14.271 kb on - strand, within GFF6829 at 14.321 kb on + strand, within GFF6829 at 14.322 kb on - strand, within GFF6829 at 14.322 kb on - strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.405 kb on + strand, within GFF6829 at 14.406 kb on - strand, within GFF6829 at 14.406 kb on - strand, within GFF6829 at 14.406 kb on - strand, within GFF6829 at 14.471 kb on + strand, within GFF6829 at 14.471 kb on + strand, within GFF6829 at 14.471 kb on + strand, within GFF6829 at 14.472 kb on - strand, within GFF6829 at 14.472 kb on - strand, within GFF6829 at 14.489 kb on + strand, within GFF6829 at 14.489 kb on + strand, within GFF6829 at 14.489 kb on + strand, within GFF6829 at 14.489 kb on + strand, within GFF6829 at 14.490 kb on - strand, within GFF6829 at 14.490 kb on - strand, within GFF6829 at 14.621 kb on + strand, within GFF6829 at 14.622 kb on - strand, within GFF6829 at 14.726 kb on + strand at 14.726 kb on + strand at 14.726 kb on + strand at 14.726 kb on + strand at 14.726 kb on + strand at 14.727 kb on - strand at 14.727 kb on - strand at 14.727 kb on - strand at 14.727 kb on - strand at 15.040 kb on - strand at 15.040 kb on - strand at 15.040 kb on - strand at 15.040 kb on - strand at 15.040 kb on - strand at 15.040 kb on - strand at 15.055 kb on - strand at 15.067 kb on - strand at 15.214 kb on - strand, within GFF6830 at 15.288 kb on + strand, within GFF6830 at 15.289 kb on - strand, within GFF6830 at 15.289 kb on - strand, within GFF6830 at 15.300 kb on + strand, within GFF6830 at 15.300 kb on + strand, within GFF6830 at 15.300 kb on + strand, within GFF6830 at 15.301 kb on - strand, within GFF6830 at 15.309 kb on + strand, within GFF6830 at 15.309 kb on + strand, within GFF6830 at 15.309 kb on + strand, within GFF6830 at 15.310 kb on - strand, within GFF6830 at 15.376 kb on + strand, within GFF6830 at 15.377 kb on - strand, within GFF6830 at 15.819 kb on + strand, within GFF6830 at 15.819 kb on + strand, within GFF6830 at 15.819 kb on + strand, within GFF6830 at 15.819 kb on + strand, within GFF6830 at 15.820 kb on - strand, within GFF6830 at 15.820 kb on - strand, within GFF6830 at 15.855 kb on + strand, within GFF6830 at 15.856 kb on - strand, within GFF6830 at 15.856 kb on - strand, within GFF6830 at 15.856 kb on - strand, within GFF6830 at 15.856 kb on - strand, within GFF6830 at 15.856 kb on - strand, within GFF6830 at 15.954 kb on + strand, within GFF6830 at 15.954 kb on + strand, within GFF6830 at 15.954 kb on + strand, within GFF6830 at 15.954 kb on + strand, within GFF6830 at 15.954 kb on + strand, within GFF6830 at 15.955 kb on - strand, within GFF6830 at 15.955 kb on - strand, within GFF6830 at 16.023 kb on + strand, within GFF6830 at 16.024 kb on - strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.182 kb on + strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.183 kb on - strand, within GFF6830 at 16.389 kb on + strand, within GFF6830 at 16.389 kb on + strand, within GFF6830 at 16.389 kb on + strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 16.390 kb on - strand, within GFF6830 at 17.064 kb on + strand at 17.064 kb on + strand at 17.064 kb on + strand at 17.064 kb on + strand at 17.064 kb on + strand at 17.065 kb on - strand at 17.065 kb on - strand at 17.065 kb on - strand at 17.065 kb on - strand at 17.065 kb on - strand at 17.065 kb on - strand at 17.076 kb on + strand at 17.076 kb on + strand at 17.076 kb on + strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.077 kb on - strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.088 kb on + strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.089 kb on - strand at 17.145 kb on + strand at 17.146 kb on - strand at 17.146 kb on - strand at 17.181 kb on + strand at 17.182 kb on - strand at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.410 kb on + strand, within GFF6831 at 17.411 kb on - strand, within GFF6831 at 17.411 kb on - strand, within GFF6831 at 17.411 kb on - strand, within GFF6831 at 17.411 kb on - strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.809 kb on + strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.810 kb on - strand, within GFF6831 at 17.956 kb on + strand at 17.956 kb on + strand at 17.957 kb on - strand at 17.957 kb on - strand at 17.957 kb on - strand at 17.957 kb on - strand at 18.025 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.037 kb on + strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.038 kb on - strand at 18.040 kb on + strand at 18.040 kb on + strand at 18.040 kb on + strand at 18.040 kb on + strand at 18.040 kb on + strand at 18.041 kb on - strand at 18.041 kb on - strand at 18.041 kb on - strand at 18.041 kb on - strand at 18.041 kb on - strand at 18.041 kb on - strand at 18.094 kb on + strand at 18.094 kb on + strand at 18.095 kb on - strand at 18.095 kb on - strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.115 kb on + strand at 18.116 kb on - strand at 18.116 kb on - strand at 18.116 kb on - strand at 18.116 kb on - strand at 18.116 kb on - strand at 18.185 kb on + strand, within GFF6832 at 18.185 kb on + strand, within GFF6832 at 18.185 kb on + strand, within GFF6832 at 18.185 kb on + strand, within GFF6832 at 18.185 kb on + strand, within GFF6832 at 18.185 kb on + strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.186 kb on - strand, within GFF6832 at 18.214 kb on + strand, within GFF6832 at 18.215 kb on - strand, within GFF6832 at 18.215 kb on - strand, within GFF6832
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 13,880 + GFF6828 0.55 -0.7 13,880 + GFF6828 0.55 -0.3 13,881 - GFF6828 0.56 -0.5 13,916 + GFF6828 0.61 -0.7 13,916 + GFF6828 0.61 +0.4 13,917 - GFF6828 0.61 -0.4 13,917 - GFF6828 0.61 -0.3 13,917 - GFF6828 0.61 -0.2 13,917 - GFF6828 0.61 -0.7 13,959 + GFF6828 0.68 +0.0 13,959 + GFF6828 0.68 +0.1 13,959 + GFF6828 0.68 -0.2 13,959 + GFF6828 0.68 +1.9 13,959 + GFF6828 0.68 -1.1 13,959 + GFF6828 0.68 +0.9 13,960 - GFF6828 0.68 +0.9 13,960 - GFF6828 0.68 -0.0 13,960 - GFF6828 0.68 +0.5 13,960 - GFF6828 0.68 -0.6 13,960 - GFF6828 0.68 +0.4 13,960 - GFF6828 0.68 +0.6 13,964 + GFF6828 0.69 -0.4 13,964 + GFF6828 0.69 +0.3 14,207 + -0.8 14,207 + -0.9 14,207 + +0.1 14,207 + -0.4 14,208 - -1.1 14,208 - +0.8 14,271 - GFF6829 0.14 +0.1 14,271 - GFF6829 0.14 +0.8 14,321 + GFF6829 0.22 -0.8 14,322 - GFF6829 0.22 -0.6 14,322 - GFF6829 0.22 -0.5 14,405 + GFF6829 0.37 +0.1 14,405 + GFF6829 0.37 -1.7 14,405 + GFF6829 0.37 -0.2 14,405 + GFF6829 0.37 -0.5 14,405 + GFF6829 0.37 +0.1 14,405 + GFF6829 0.37 +0.2 14,405 + GFF6829 0.37 -0.1 14,405 + GFF6829 0.37 -0.2 14,405 + GFF6829 0.37 +0.5 14,405 + GFF6829 0.37 +2.9 14,405 + GFF6829 0.37 -1.9 14,405 + GFF6829 0.37 -0.3 14,405 + GFF6829 0.37 +0.3 14,406 - GFF6829 0.37 +0.7 14,406 - GFF6829 0.37 +0.1 14,406 - GFF6829 0.37 -2.2 14,471 + GFF6829 0.48 +0.9 14,471 + GFF6829 0.48 +1.3 14,471 + GFF6829 0.48 -0.4 14,472 - GFF6829 0.48 +0.7 14,472 - GFF6829 0.48 +0.5 14,489 + GFF6829 0.51 -0.8 14,489 + GFF6829 0.51 -0.5 14,489 + GFF6829 0.51 -0.5 14,489 + GFF6829 0.51 -0.3 14,490 - GFF6829 0.51 +2.3 14,490 - GFF6829 0.51 -1.4 14,621 + GFF6829 0.74 -0.4 14,622 - GFF6829 0.74 -0.6 14,726 + +0.8 14,726 + -0.3 14,726 + -0.6 14,726 + +0.6 14,726 + -0.3 14,727 - +0.7 14,727 - +0.3 14,727 - -0.3 14,727 - +0.9 15,040 - -0.9 15,040 - -0.4 15,040 - +0.3 15,040 - +1.5 15,040 - +0.6 15,040 - -0.9 15,055 - -0.3 15,067 - +0.5 15,214 - GFF6830 0.14 -1.4 15,288 + GFF6830 0.17 -1.0 15,289 - GFF6830 0.17 -0.2 15,289 - GFF6830 0.17 +0.1 15,300 + GFF6830 0.18 -0.1 15,300 + GFF6830 0.18 +0.1 15,300 + GFF6830 0.18 -0.6 15,301 - GFF6830 0.18 -0.1 15,309 + GFF6830 0.18 -0.6 15,309 + GFF6830 0.18 +0.5 15,309 + GFF6830 0.18 -0.1 15,310 - GFF6830 0.18 -0.4 15,376 + GFF6830 0.21 -0.4 15,377 - GFF6830 0.21 -0.7 15,819 + GFF6830 0.39 -1.2 15,819 + GFF6830 0.39 -1.5 15,819 + GFF6830 0.39 -0.3 15,819 + GFF6830 0.39 -1.5 15,820 - GFF6830 0.39 -0.6 15,820 - GFF6830 0.39 -0.4 15,855 + GFF6830 0.41 -0.5 15,856 - GFF6830 0.41 +0.9 15,856 - GFF6830 0.41 -0.6 15,856 - GFF6830 0.41 +0.5 15,856 - GFF6830 0.41 -1.2 15,856 - GFF6830 0.41 -0.5 15,954 + GFF6830 0.45 -0.9 15,954 + GFF6830 0.45 +0.5 15,954 + GFF6830 0.45 -1.5 15,954 + GFF6830 0.45 -1.0 15,954 + GFF6830 0.45 -0.7 15,955 - GFF6830 0.45 +1.3 15,955 - GFF6830 0.45 -1.2 16,023 + GFF6830 0.48 -0.7 16,024 - GFF6830 0.48 -0.2 16,182 + GFF6830 0.54 -0.8 16,182 + GFF6830 0.54 +0.3 16,182 + GFF6830 0.54 +0.2 16,182 + GFF6830 0.54 +0.1 16,182 + GFF6830 0.54 +2.9 16,182 + GFF6830 0.54 -0.3 16,182 + GFF6830 0.54 -0.2 16,182 + GFF6830 0.54 +1.1 16,182 + GFF6830 0.54 +0.3 16,183 - GFF6830 0.54 -1.5 16,183 - GFF6830 0.54 -0.8 16,183 - GFF6830 0.54 +0.3 16,183 - GFF6830 0.54 -1.5 16,183 - GFF6830 0.54 +1.3 16,183 - GFF6830 0.54 -0.8 16,183 - GFF6830 0.54 +0.7 16,183 - GFF6830 0.54 -0.3 16,389 + GFF6830 0.63 -1.0 16,389 + GFF6830 0.63 -0.4 16,389 + GFF6830 0.63 -0.8 16,390 - GFF6830 0.63 -0.4 16,390 - GFF6830 0.63 -1.1 16,390 - GFF6830 0.63 -0.5 16,390 - GFF6830 0.63 -0.7 16,390 - GFF6830 0.63 -0.2 16,390 - GFF6830 0.63 +0.2 17,064 + -0.1 17,064 + -0.0 17,064 + -0.5 17,064 + -0.1 17,064 + -1.2 17,065 - -0.5 17,065 - -0.1 17,065 - -0.6 17,065 - -0.5 17,065 - -0.7 17,065 - +0.2 17,076 + -0.3 17,076 + -0.7 17,076 + -0.6 17,077 - -1.5 17,077 - +0.9 17,077 - -1.7 17,077 - -1.0 17,077 - -1.0 17,077 - -0.6 17,088 + -0.8 17,088 + -0.3 17,088 + +0.3 17,088 + -0.7 17,088 + -1.3 17,088 + +1.3 17,088 + +1.9 17,088 + -0.3 17,088 + -0.4 17,088 + -0.5 17,088 + +0.3 17,088 + -0.9 17,088 + -0.3 17,089 - -0.4 17,089 - +0.4 17,089 - -1.7 17,089 - -0.0 17,089 - -0.7 17,089 - +0.3 17,089 - -0.5 17,145 + +0.6 17,146 - -0.3 17,146 - +0.5 17,181 + -1.9 17,182 - -0.2 17,410 + GFF6831 0.16 -0.5 17,410 + GFF6831 0.16 -2.0 17,410 + GFF6831 0.16 -0.6 17,410 + GFF6831 0.16 -1.1 17,410 + GFF6831 0.16 -0.1 17,410 + GFF6831 0.16 -0.1 17,410 + GFF6831 0.16 -1.3 17,411 - GFF6831 0.16 -0.3 17,411 - GFF6831 0.16 +0.3 17,411 - GFF6831 0.16 +0.0 17,411 - GFF6831 0.16 -0.3 17,809 + GFF6831 0.77 -1.4 17,809 + GFF6831 0.77 -0.7 17,809 + GFF6831 0.77 +0.4 17,809 + GFF6831 0.77 -0.9 17,809 + GFF6831 0.77 +0.6 17,809 + GFF6831 0.77 -0.5 17,809 + GFF6831 0.77 -0.8 17,810 - GFF6831 0.77 -2.2 17,810 - GFF6831 0.77 -1.5 17,810 - GFF6831 0.77 -1.2 17,810 - GFF6831 0.77 -0.0 17,810 - GFF6831 0.77 +0.7 17,810 - GFF6831 0.77 -1.7 17,810 - GFF6831 0.77 -0.5 17,956 + -1.2 17,956 + +0.4 17,957 - +0.1 17,957 - -0.0 17,957 - +0.6 17,957 - -0.2 18,025 + -1.0 18,037 + +0.5 18,037 + -0.8 18,037 + -0.2 18,037 + +1.4 18,037 + -2.0 18,037 + -1.3 18,037 + +0.8 18,037 + -1.6 18,037 + +1.2 18,037 + -0.3 18,038 - -0.6 18,038 - -0.6 18,038 - -0.3 18,038 - -1.0 18,038 - -0.5 18,038 - +0.9 18,038 - -0.5 18,040 + +0.1 18,040 + +0.1 18,040 + -0.2 18,040 + -0.0 18,040 + -0.2 18,041 - +0.3 18,041 - +0.4 18,041 - -1.8 18,041 - -0.5 18,041 - -0.8 18,041 - -1.3 18,094 + +1.0 18,094 + +0.4 18,095 - +1.3 18,095 - -1.9 18,115 + -0.4 18,115 + +0.2 18,115 + +0.2 18,115 + -0.7 18,115 + -0.3 18,115 + -0.9 18,115 + +1.9 18,116 - -0.5 18,116 - -1.0 18,116 - -0.1 18,116 - -0.5 18,116 - +1.5 18,185 + GFF6832 0.17 +0.4 18,185 + GFF6832 0.17 +0.5 18,185 + GFF6832 0.17 -0.6 18,185 + GFF6832 0.17 -1.3 18,185 + GFF6832 0.17 +1.0 18,185 + GFF6832 0.17 -0.0 18,186 - GFF6832 0.17 -0.0 18,186 - GFF6832 0.17 +0.1 18,186 - GFF6832 0.17 -0.3 18,186 - GFF6832 0.17 -0.5 18,186 - GFF6832 0.17 +0.4 18,186 - GFF6832 0.17 -0.2 18,214 + GFF6832 0.20 -0.3 18,215 - GFF6832 0.20 -1.2 18,215 - GFF6832 0.20 +1.0
Or see this region's nucleotide sequence