Strain Fitness in Variovorax sp. SCN45 around GFF6506

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
657	+		GFF6504	0.39	+0.0
657	+		GFF6504	0.39	-0.2
657	+		GFF6504	0.39	+1.1
658	-		GFF6504	0.39	+0.8
658	-		GFF6504	0.39	-0.5
779	-		GFF6504	0.55	+0.3
795	+		GFF6504	0.58	-1.5
795	+		GFF6504	0.58	-1.3
796	-		GFF6504	0.58	-0.7
796	-		GFF6504	0.58	-0.4
796	-		GFF6504	0.58	-0.4
796	-		GFF6504	0.58	-0.2
796	-		GFF6504	0.58	-1.1
932	+		GFF6504	0.76	+1.5
933	-		GFF6504	0.76	-0.3
987	-		GFF6504	0.83	-0.6
987	-		GFF6504	0.83	+0.0
988	+		GFF6504	0.83	-0.4
988	+		GFF6504	0.83	+0.8
988	+		GFF6504	0.83	+0.8
988	+		GFF6504	0.83	-0.2
988	+		GFF6504	0.83	-0.8
988	+		GFF6504	0.83	-0.2
988	+		GFF6504	0.83	+0.2
989	-		GFF6504	0.84	-0.9
989	-		GFF6504	0.84	-1.2
989	-		GFF6504	0.84	+2.8
989	-		GFF6504	0.84	+0.0
989	-		GFF6504	0.84	-0.2
989	-		GFF6504	0.84	+0.3
994	-		GFF6504	0.84	-0.7
1,045	+				-2.3
1,046	-				+0.1
1,099	+				-0.1
1,339	-		GFF6505	0.54	-3.0
1,339	-		GFF6505	0.54	-1.7
1,582	-				-0.3
1,596	-				+2.5
1,613	+				-0.4
1,643	+				+0.1
1,644	-				-0.1
1,644	-				-0.1
1,644	-				-0.5
1,906	+		GFF6506	0.85	-0.3
1,906	+		GFF6506	0.85	-1.0
1,906	+		GFF6506	0.85	-0.2
1,906	+		GFF6506	0.85	+0.2
1,907	-		GFF6506	0.85	-1.1
1,907	-		GFF6506	0.85	-0.9
1,972	+				+0.5
1,973	-				-1.8
1,990	+				-0.3
2,003	+				+0.4
2,003	+				+0.0
2,004	-				-0.2
2,004	-				+0.3
2,004	-				-1.7
2,105	+				-0.0
2,105	+				-0.9
2,106	-				+0.5
2,125	+				-0.8
2,126	-				+1.2
2,142	+				+0.8
2,142	+				-2.1
2,152	+				-2.6
2,152	+				-0.3
2,153	-				-0.1
2,181	+				-0.9
2,182	-				+0.3
2,182	-				-1.1
2,182	-				+0.0
2,182	-				+1.0
2,261	-				-1.1
2,271	+		GFF6507	0.15	+0.2
2,303	+		GFF6507	0.38	-0.1
2,303	+		GFF6507	0.38	+0.8
2,303	+		GFF6507	0.38	-0.1
2,303	+		GFF6507	0.38	-0.8
2,304	-		GFF6507	0.38	-0.6
2,304	-		GFF6507	0.38	+1.7
2,305	+		GFF6507	0.39	+1.4
2,305	+		GFF6507	0.39	-1.4
2,369	+		GFF6507	0.83	-0.0
2,370	-		GFF6507	0.84	-0.7
2,370	-		GFF6507	0.84	-1.2
2,429	+				-0.3
2,429	+				-0.4
2,429	+				-1.8
2,429	+				-1.0
2,430	-				-0.2
2,430	-				+0.3
2,430	-				+0.4
2,430	-				+0.5
2,439	+				-0.8
2,439	+				-0.1
2,440	-				-0.3
2,440	-				+0.0
2,453	+				-0.2
2,454	-				+1.5
2,512	+				+0.5
2,512	+				+0.7
2,512	+				+0.9
2,536	+				+0.0
2,536	+				-0.9
2,536	+				-0.5
2,536	+				+0.2
2,536	+				+0.7
2,536	+				+0.3
2,537	-				-0.3
2,537	-				-0.9
2,618	+				-1.3
2,618	+				+0.1
2,618	+				+0.3
2,619	-				-0.1
2,619	-				-1.5
2,619	-				-2.7
2,619	-				-0.2
2,661	-				-0.8
2,706	+				-0.7
2,773	+		GFF6508	0.12	+1.8
2,774	-		GFF6508	0.12	+0.4
2,774	-		GFF6508	0.12	-0.4
2,774	-		GFF6508	0.12	-0.3
2,774	-		GFF6508	0.12	-0.4
2,774	-		GFF6508	0.12	+1.1
2,862	+		GFF6508	0.19	-1.0
2,862	+		GFF6508	0.19	+0.4
2,862	+		GFF6508	0.19	-0.3
2,862	+		GFF6508	0.19	+0.2
2,863	-		GFF6508	0.19	+0.3
2,863	-		GFF6508	0.19	-0.5