Strain Fitness in Variovorax sp. SCN45 around GFF6498

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6497 and GFF6498 are separated by 140 nucleotidesGFF6498 and GFF6499 are separated by 5 nucleotidesGFF6499 and GFF6500 are separated by 122 nucleotides GFF6497 - Protein of unknown function (DUF1501), at 73,419 to 74,630 GFF6497 GFF6498 - FIG00348192: hypothetical protein, at 74,771 to 76,363 GFF6498 GFF6499 - hypothetical protein, at 76,369 to 76,848 GFF6499 GFF6500 - 2-hydroxychromene-2-carboxylate isomerase/DsbA-like thioredoxin domain, at 76,971 to 77,657 GFF6500 Position (kb) 74 75 76 77Strain fitness (log2 ratio) -2 -1 0 1 2at 73.807 kb on + strand, within GFF6497at 73.808 kb on - strand, within GFF6497at 73.808 kb on - strand, within GFF6497at 73.808 kb on - strand, within GFF6497at 73.808 kb on - strand, within GFF6497at 73.808 kb on - strand, within GFF6497at 74.038 kb on + strand, within GFF6497at 74.038 kb on + strand, within GFF6497at 74.038 kb on + strand, within GFF6497at 74.038 kb on + strand, within GFF6497at 74.039 kb on - strand, within GFF6497at 74.039 kb on - strand, within GFF6497at 74.039 kb on - strand, within GFF6497at 74.039 kb on - strand, within GFF6497at 74.230 kb on + strand, within GFF6497at 74.461 kb on + strand, within GFF6497at 74.462 kb on - strand, within GFF6497at 74.462 kb on - strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.464 kb on + strand, within GFF6497at 74.506 kb on + strand, within GFF6497at 74.759 kb on + strandat 74.759 kb on + strandat 74.759 kb on + strandat 74.766 kb on + strandat 74.767 kb on - strandat 74.767 kb on - strandat 75.069 kb on + strand, within GFF6498at 75.069 kb on + strand, within GFF6498at 75.069 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.165 kb on + strand, within GFF6498at 75.166 kb on - strand, within GFF6498at 75.166 kb on - strand, within GFF6498at 75.166 kb on - strand, within GFF6498at 75.166 kb on - strand, within GFF6498at 75.321 kb on + strand, within GFF6498at 75.321 kb on + strand, within GFF6498at 75.447 kb on + strand, within GFF6498at 75.447 kb on + strand, within GFF6498at 75.448 kb on - strand, within GFF6498at 75.448 kb on - strand, within GFF6498at 75.492 kb on + strand, within GFF6498at 75.492 kb on + strand, within GFF6498at 75.492 kb on + strand, within GFF6498at 75.493 kb on - strand, within GFF6498at 75.493 kb on - strand, within GFF6498at 75.652 kb on - strand, within GFF6498at 75.783 kb on + strand, within GFF6498at 75.945 kb on + strand, within GFF6498at 75.945 kb on + strand, within GFF6498at 75.946 kb on - strand, within GFF6498at 75.946 kb on - strand, within GFF6498at 75.946 kb on - strand, within GFF6498at 76.748 kb on + strand, within GFF6499at 76.749 kb on - strand, within GFF6499at 76.767 kb on - strand, within GFF6499at 76.832 kb on - strandat 76.956 kb on + strandat 76.967 kb on + strandat 76.967 kb on + strandat 76.967 kb on + strandat 76.967 kb on + strandat 76.968 kb on - strandat 77.113 kb on + strand, within GFF6500at 77.113 kb on + strand, within GFF6500at 77.113 kb on + strand, within GFF6500at 77.113 kb on + strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.114 kb on - strand, within GFF6500at 77.254 kb on + strand, within GFF6500at 77.254 kb on + strand, within GFF6500at 77.255 kb on - strand, within GFF6500at 77.347 kb on + strand, within GFF6500at 77.347 kb on + strand, within GFF6500at 77.347 kb on + strand, within GFF6500at 77.347 kb on + strand, within GFF6500

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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73,807 + GFF6497 0.32 -0.2
73,808 - GFF6497 0.32 -0.8
73,808 - GFF6497 0.32 -0.9
73,808 - GFF6497 0.32 -1.8
73,808 - GFF6497 0.32 -0.1
73,808 - GFF6497 0.32 +0.3
74,038 + GFF6497 0.51 -0.3
74,038 + GFF6497 0.51 -0.1
74,038 + GFF6497 0.51 -1.5
74,038 + GFF6497 0.51 -0.1
74,039 - GFF6497 0.51 -2.1
74,039 - GFF6497 0.51 +2.3
74,039 - GFF6497 0.51 +0.7
74,039 - GFF6497 0.51 -0.2
74,230 + GFF6497 0.67 -0.6
74,461 + GFF6497 0.86 -1.4
74,462 - GFF6497 0.86 +0.2
74,462 - GFF6497 0.86 -0.1
74,464 + GFF6497 0.86 -0.6
74,464 + GFF6497 0.86 +0.1
74,464 + GFF6497 0.86 -1.1
74,464 + GFF6497 0.86 +0.0
74,464 + GFF6497 0.86 -0.2
74,464 + GFF6497 0.86 -0.6
74,506 + GFF6497 0.90 -2.6
74,759 + -2.1
74,759 + -0.5
74,759 + -0.3
74,766 + +0.7
74,767 - +0.1
74,767 - -1.8
75,069 + GFF6498 0.19 +1.2
75,069 + GFF6498 0.19 +0.5
75,069 + GFF6498 0.19 +1.0
75,165 + GFF6498 0.25 -1.1
75,165 + GFF6498 0.25 +0.2
75,165 + GFF6498 0.25 +0.5
75,165 + GFF6498 0.25 +0.3
75,165 + GFF6498 0.25 +0.9
75,165 + GFF6498 0.25 -0.3
75,165 + GFF6498 0.25 -1.3
75,166 - GFF6498 0.25 +0.1
75,166 - GFF6498 0.25 +0.3
75,166 - GFF6498 0.25 -1.1
75,166 - GFF6498 0.25 +0.7
75,321 + GFF6498 0.35 -0.5
75,321 + GFF6498 0.35 -1.3
75,447 + GFF6498 0.42 -0.7
75,447 + GFF6498 0.42 -1.5
75,448 - GFF6498 0.42 -0.1
75,448 - GFF6498 0.42 +2.0
75,492 + GFF6498 0.45 +0.6
75,492 + GFF6498 0.45 +0.1
75,492 + GFF6498 0.45 -0.5
75,493 - GFF6498 0.45 -0.1
75,493 - GFF6498 0.45 -0.4
75,652 - GFF6498 0.55 +0.1
75,783 + GFF6498 0.64 -0.1
75,945 + GFF6498 0.74 +0.2
75,945 + GFF6498 0.74 -0.6
75,946 - GFF6498 0.74 +0.1
75,946 - GFF6498 0.74 +0.4
75,946 - GFF6498 0.74 -0.1
76,748 + GFF6499 0.79 -0.4
76,749 - GFF6499 0.79 +0.9
76,767 - GFF6499 0.83 +1.2
76,832 - -0.5
76,956 + -0.4
76,967 + -1.5
76,967 + +0.5
76,967 + -1.1
76,967 + -0.8
76,968 - -0.9
77,113 + GFF6500 0.21 -0.3
77,113 + GFF6500 0.21 +0.6
77,113 + GFF6500 0.21 -0.0
77,113 + GFF6500 0.21 -1.4
77,114 - GFF6500 0.21 -0.1
77,114 - GFF6500 0.21 +0.5
77,114 - GFF6500 0.21 -0.7
77,114 - GFF6500 0.21 -1.2
77,114 - GFF6500 0.21 +0.4
77,114 - GFF6500 0.21 -1.4
77,114 - GFF6500 0.21 +0.5
77,114 - GFF6500 0.21 -0.2
77,254 + GFF6500 0.41 -0.4
77,254 + GFF6500 0.41 -0.1
77,255 - GFF6500 0.41 +0.2
77,347 + GFF6500 0.55 -0.1
77,347 + GFF6500 0.55 -0.3
77,347 + GFF6500 0.55 +0.0
77,347 + GFF6500 0.55 -0.2

Or see this region's nucleotide sequence