Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6410 and GFF6411 are separated by 11 nucleotides GFF6411 and GFF6412 overlap by 4 nucleotides GFF6412 and GFF6413 are separated by 13 nucleotides
GFF6410 - Histidine utilization repressor, at 85,270 to 86,007
GFF6410
GFF6411 - Histidine ABC transporter, ATP-binding protein, at 86,019 to 86,801
GFF6411
GFF6412 - Histidine ABC transporter, permease protein, at 86,798 to 87,640
GFF6412
GFF6413 - Histidine ABC transporter, substrate-binding protein, at 87,654 to 88,625
GFF6413
Position (kb)
86
87
88 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 85.976 kb on + strand at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.110 kb on + strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.111 kb on - strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.542 kb on + strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.543 kb on - strand, within GFF6411 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.982 kb on + strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 86.983 kb on - strand, within GFF6412 at 87.090 kb on + strand, within GFF6412 at 87.090 kb on + strand, within GFF6412 at 87.090 kb on + strand, within GFF6412 at 87.090 kb on + strand, within GFF6412 at 87.171 kb on + strand, within GFF6412 at 87.249 kb on + strand, within GFF6412 at 87.249 kb on + strand, within GFF6412 at 87.308 kb on - strand, within GFF6412 at 87.308 kb on - strand, within GFF6412 at 87.399 kb on + strand, within GFF6412 at 87.400 kb on - strand, within GFF6412 at 87.497 kb on + strand, within GFF6412 at 87.497 kb on + strand, within GFF6412 at 87.497 kb on + strand, within GFF6412 at 87.497 kb on + strand, within GFF6412 at 87.497 kb on + strand, within GFF6412 at 87.498 kb on - strand, within GFF6412 at 87.498 kb on - strand, within GFF6412 at 87.498 kb on - strand, within GFF6412 at 87.498 kb on - strand, within GFF6412 at 87.498 kb on - strand, within GFF6412 at 87.655 kb on + strand at 87.655 kb on + strand at 87.655 kb on + strand at 87.655 kb on + strand at 87.655 kb on + strand at 87.655 kb on + strand at 87.655 kb on + strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.656 kb on - strand at 87.873 kb on - strand, within GFF6413 at 87.916 kb on + strand, within GFF6413 at 87.916 kb on + strand, within GFF6413 at 87.916 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.060 kb on + strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.061 kb on - strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.069 kb on + strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.070 kb on - strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.195 kb on + strand, within GFF6413 at 88.196 kb on - strand, within GFF6413 at 88.196 kb on - strand, within GFF6413 at 88.291 kb on + strand, within GFF6413 at 88.292 kb on - strand, within GFF6413 at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.608 kb on + strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.609 kb on - strand at 88.629 kb on + strand at 88.629 kb on + strand at 88.629 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 85,976 + +0.9 86,110 + GFF6411 0.12 -0.3 86,110 + GFF6411 0.12 +0.6 86,110 + GFF6411 0.12 +0.4 86,110 + GFF6411 0.12 -0.6 86,110 + GFF6411 0.12 +0.5 86,111 - GFF6411 0.12 -0.8 86,111 - GFF6411 0.12 -1.3 86,111 - GFF6411 0.12 -0.2 86,111 - GFF6411 0.12 -0.8 86,111 - GFF6411 0.12 +1.5 86,111 - GFF6411 0.12 -0.6 86,542 + GFF6411 0.67 -0.3 86,542 + GFF6411 0.67 -0.5 86,542 + GFF6411 0.67 +0.8 86,542 + GFF6411 0.67 +1.3 86,542 + GFF6411 0.67 -1.2 86,543 - GFF6411 0.67 -0.5 86,543 - GFF6411 0.67 +0.1 86,543 - GFF6411 0.67 -0.3 86,982 + GFF6412 0.22 +0.3 86,982 + GFF6412 0.22 -0.2 86,982 + GFF6412 0.22 +1.4 86,982 + GFF6412 0.22 -0.6 86,982 + GFF6412 0.22 +0.2 86,982 + GFF6412 0.22 -0.5 86,982 + GFF6412 0.22 -1.1 86,982 + GFF6412 0.22 -0.1 86,983 - GFF6412 0.22 +0.3 86,983 - GFF6412 0.22 -0.6 86,983 - GFF6412 0.22 +0.0 86,983 - GFF6412 0.22 +0.1 86,983 - GFF6412 0.22 -0.1 86,983 - GFF6412 0.22 +0.4 87,090 + GFF6412 0.35 +0.6 87,090 + GFF6412 0.35 -0.1 87,090 + GFF6412 0.35 -0.4 87,090 + GFF6412 0.35 +1.1 87,171 + GFF6412 0.44 -1.1 87,249 + GFF6412 0.53 +0.6 87,249 + GFF6412 0.53 +0.2 87,308 - GFF6412 0.60 +0.0 87,308 - GFF6412 0.60 -0.1 87,399 + GFF6412 0.71 -1.1 87,400 - GFF6412 0.71 -0.5 87,497 + GFF6412 0.83 -0.2 87,497 + GFF6412 0.83 -0.2 87,497 + GFF6412 0.83 +1.3 87,497 + GFF6412 0.83 -1.0 87,497 + GFF6412 0.83 -0.1 87,498 - GFF6412 0.83 -2.0 87,498 - GFF6412 0.83 -0.3 87,498 - GFF6412 0.83 -1.9 87,498 - GFF6412 0.83 +0.5 87,498 - GFF6412 0.83 +1.0 87,655 + +0.5 87,655 + -0.5 87,655 + +0.3 87,655 + +0.5 87,655 + -0.7 87,655 + +0.9 87,655 + -0.6 87,656 - +1.9 87,656 - -0.4 87,656 - +0.4 87,656 - -0.4 87,656 - +1.1 87,656 - -1.2 87,656 - -0.7 87,656 - -0.4 87,656 - +0.1 87,656 - -0.7 87,656 - -0.3 87,656 - -0.9 87,656 - +1.5 87,873 - GFF6413 0.23 +1.5 87,916 + GFF6413 0.27 +0.1 87,916 + GFF6413 0.27 +1.4 87,916 + GFF6413 0.27 +0.2 88,060 + GFF6413 0.42 +0.0 88,060 + GFF6413 0.42 +0.1 88,060 + GFF6413 0.42 +1.9 88,060 + GFF6413 0.42 -0.3 88,061 - GFF6413 0.42 -0.2 88,061 - GFF6413 0.42 -0.2 88,061 - GFF6413 0.42 +0.5 88,061 - GFF6413 0.42 -0.1 88,061 - GFF6413 0.42 +0.1 88,069 + GFF6413 0.43 -0.6 88,069 + GFF6413 0.43 +0.2 88,069 + GFF6413 0.43 -0.1 88,069 + GFF6413 0.43 -0.1 88,069 + GFF6413 0.43 -2.1 88,069 + GFF6413 0.43 +0.4 88,069 + GFF6413 0.43 +1.5 88,069 + GFF6413 0.43 -1.5 88,069 + GFF6413 0.43 -0.8 88,070 - GFF6413 0.43 +0.9 88,070 - GFF6413 0.43 +0.2 88,070 - GFF6413 0.43 -0.6 88,070 - GFF6413 0.43 +0.6 88,070 - GFF6413 0.43 -0.0 88,070 - GFF6413 0.43 -0.1 88,070 - GFF6413 0.43 -0.6 88,070 - GFF6413 0.43 -0.2 88,070 - GFF6413 0.43 -1.5 88,070 - GFF6413 0.43 +1.3 88,070 - GFF6413 0.43 +1.1 88,070 - GFF6413 0.43 -1.9 88,070 - GFF6413 0.43 +0.7 88,070 - GFF6413 0.43 -0.4 88,070 - GFF6413 0.43 -0.8 88,070 - GFF6413 0.43 +1.9 88,195 + GFF6413 0.56 -0.6 88,195 + GFF6413 0.56 -0.2 88,195 + GFF6413 0.56 -0.1 88,196 - GFF6413 0.56 -0.1 88,196 - GFF6413 0.56 -1.2 88,291 + GFF6413 0.66 -1.3 88,292 - GFF6413 0.66 -0.1 88,608 + -0.2 88,608 + -1.8 88,608 + -0.0 88,608 + +0.7 88,608 + -1.8 88,608 + -0.4 88,608 + +0.1 88,608 + +0.6 88,609 - +0.2 88,609 - +0.4 88,609 - +0.2 88,609 - +0.4 88,609 - -0.1 88,609 - +0.5 88,629 + +0.8 88,629 + -0.6 88,629 + -0.2
Or see this region's nucleotide sequence