Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6406 and GFF6407 are separated by 53 nucleotides GFF6407 and GFF6408 overlap by 1 nucleotides GFF6408 and GFF6409 are separated by 30 nucleotides
GFF6406 - Transcriptional regulator, IclR family, at 80,186 to 80,992
GFF6406
GFF6407 - Phytanoyl-CoA dioxygenase, at 81,046 to 81,891
GFF6407
GFF6408 - Urocanate hydratase (EC 4.2.1.49), at 81,891 to 83,618
GFF6408
GFF6409 - Histidine ammonia-lyase (EC 4.3.1.3), at 83,649 to 85,208
GFF6409
Position (kb)
81
82
83
84 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 80.966 kb on + strand at 80.966 kb on + strand at 80.967 kb on - strand at 80.967 kb on - strand at 80.967 kb on - strand at 80.967 kb on - strand at 80.967 kb on - strand at 80.967 kb on - strand at 81.018 kb on - strand at 81.036 kb on + strand at 81.036 kb on + strand at 81.036 kb on + strand at 81.036 kb on + strand at 81.037 kb on - strand at 81.037 kb on - strand at 81.037 kb on - strand at 81.037 kb on - strand at 81.037 kb on - strand at 81.048 kb on - strand at 81.368 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.488 kb on + strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.489 kb on - strand, within GFF6407 at 81.491 kb on + strand, within GFF6407 at 81.491 kb on + strand, within GFF6407 at 81.491 kb on + strand, within GFF6407 at 81.492 kb on - strand, within GFF6407 at 81.492 kb on - strand, within GFF6407 at 81.492 kb on - strand, within GFF6407 at 81.492 kb on - strand, within GFF6407 at 81.560 kb on + strand, within GFF6407 at 81.560 kb on + strand, within GFF6407 at 81.561 kb on - strand, within GFF6407 at 82.070 kb on - strand, within GFF6408 at 82.070 kb on - strand, within GFF6408 at 82.070 kb on - strand, within GFF6408 at 82.070 kb on - strand, within GFF6408 at 82.070 kb on - strand, within GFF6408 at 82.070 kb on - strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.441 kb on + strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.442 kb on - strand, within GFF6408 at 82.516 kb on + strand, within GFF6408 at 82.516 kb on + strand, within GFF6408 at 82.516 kb on + strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.517 kb on - strand, within GFF6408 at 82.747 kb on + strand, within GFF6408 at 82.748 kb on - strand, within GFF6408 at 82.948 kb on + strand, within GFF6408 at 82.948 kb on + strand, within GFF6408 at 82.948 kb on + strand, within GFF6408 at 82.949 kb on - strand, within GFF6408 at 83.097 kb on - strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.098 kb on + strand, within GFF6408 at 83.099 kb on - strand, within GFF6408 at 83.099 kb on - strand, within GFF6408 at 83.099 kb on - strand, within GFF6408 at 83.099 kb on - strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.164 kb on + strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.165 kb on - strand, within GFF6408 at 83.194 kb on + strand, within GFF6408 at 83.194 kb on + strand, within GFF6408 at 83.195 kb on - strand, within GFF6408 at 83.195 kb on - strand, within GFF6408 at 83.386 kb on + strand, within GFF6408 at 83.386 kb on + strand, within GFF6408 at 83.386 kb on + strand, within GFF6408 at 83.386 kb on + strand, within GFF6408 at 83.387 kb on - strand, within GFF6408 at 83.387 kb on - strand, within GFF6408 at 83.387 kb on - strand, within GFF6408 at 83.425 kb on + strand, within GFF6408 at 83.603 kb on + strand at 83.603 kb on + strand at 83.604 kb on - strand at 83.604 kb on - strand at 83.605 kb on + strand at 83.606 kb on - strand at 83.632 kb on + strand at 83.632 kb on + strand at 83.632 kb on + strand at 83.632 kb on + strand at 83.633 kb on - strand at 83.633 kb on - strand at 83.633 kb on - strand at 83.651 kb on - strand at 83.740 kb on + strand at 83.740 kb on + strand at 83.741 kb on - strand at 84.209 kb on + strand, within GFF6409 at 84.209 kb on + strand, within GFF6409 at 84.209 kb on + strand, within GFF6409 at 84.210 kb on - strand, within GFF6409 at 84.210 kb on - strand, within GFF6409 at 84.210 kb on - strand, within GFF6409 at 84.579 kb on + strand, within GFF6409
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 80,966 + +0.3 80,966 + +1.4 80,967 - +1.0 80,967 - +1.6 80,967 - +0.7 80,967 - +0.4 80,967 - -0.9 80,967 - +0.1 81,018 - +0.2 81,036 + +0.5 81,036 + -0.2 81,036 + -0.2 81,036 + -1.1 81,037 - -0.8 81,037 - +0.5 81,037 - +0.5 81,037 - +0.4 81,037 - -0.3 81,048 - -1.1 81,368 + GFF6407 0.38 -0.8 81,488 + GFF6407 0.52 +1.4 81,488 + GFF6407 0.52 +0.2 81,488 + GFF6407 0.52 +0.9 81,488 + GFF6407 0.52 +0.5 81,488 + GFF6407 0.52 -0.2 81,488 + GFF6407 0.52 +2.5 81,489 - GFF6407 0.52 +0.8 81,489 - GFF6407 0.52 +0.1 81,489 - GFF6407 0.52 +0.5 81,489 - GFF6407 0.52 -1.4 81,489 - GFF6407 0.52 -0.4 81,489 - GFF6407 0.52 +2.4 81,489 - GFF6407 0.52 -0.0 81,489 - GFF6407 0.52 +0.7 81,489 - GFF6407 0.52 +0.6 81,491 + GFF6407 0.53 -0.1 81,491 + GFF6407 0.53 -1.1 81,491 + GFF6407 0.53 -0.1 81,492 - GFF6407 0.53 -1.0 81,492 - GFF6407 0.53 +0.2 81,492 - GFF6407 0.53 +0.1 81,492 - GFF6407 0.53 +0.1 81,560 + GFF6407 0.61 -0.0 81,560 + GFF6407 0.61 -0.6 81,561 - GFF6407 0.61 -0.5 82,070 - GFF6408 0.10 -0.4 82,070 - GFF6408 0.10 -1.1 82,070 - GFF6408 0.10 -0.6 82,070 - GFF6408 0.10 -0.9 82,070 - GFF6408 0.10 +0.1 82,070 - GFF6408 0.10 -0.1 82,441 + GFF6408 0.32 -0.8 82,441 + GFF6408 0.32 -1.9 82,441 + GFF6408 0.32 +0.7 82,441 + GFF6408 0.32 -0.4 82,441 + GFF6408 0.32 -0.6 82,441 + GFF6408 0.32 -0.0 82,442 - GFF6408 0.32 +0.1 82,442 - GFF6408 0.32 +0.1 82,442 - GFF6408 0.32 +0.1 82,442 - GFF6408 0.32 +0.2 82,442 - GFF6408 0.32 -0.1 82,442 - GFF6408 0.32 -0.1 82,516 + GFF6408 0.36 -1.5 82,516 + GFF6408 0.36 +0.5 82,516 + GFF6408 0.36 +0.4 82,517 - GFF6408 0.36 -2.6 82,517 - GFF6408 0.36 -0.2 82,517 - GFF6408 0.36 -0.2 82,517 - GFF6408 0.36 -0.8 82,517 - GFF6408 0.36 +0.9 82,517 - GFF6408 0.36 +0.6 82,747 + GFF6408 0.50 -1.1 82,748 - GFF6408 0.50 -0.1 82,948 + GFF6408 0.61 +1.7 82,948 + GFF6408 0.61 -0.3 82,948 + GFF6408 0.61 -0.5 82,949 - GFF6408 0.61 +0.7 83,097 - GFF6408 0.70 -0.3 83,098 + GFF6408 0.70 +0.1 83,098 + GFF6408 0.70 +1.3 83,098 + GFF6408 0.70 +1.7 83,098 + GFF6408 0.70 +1.9 83,098 + GFF6408 0.70 +0.5 83,098 + GFF6408 0.70 -0.7 83,099 - GFF6408 0.70 -1.5 83,099 - GFF6408 0.70 +0.1 83,099 - GFF6408 0.70 -0.3 83,099 - GFF6408 0.70 -0.2 83,164 + GFF6408 0.74 +0.5 83,164 + GFF6408 0.74 -1.0 83,164 + GFF6408 0.74 +0.5 83,164 + GFF6408 0.74 +0.2 83,164 + GFF6408 0.74 -0.4 83,164 + GFF6408 0.74 +0.2 83,164 + GFF6408 0.74 -1.2 83,165 - GFF6408 0.74 -0.2 83,165 - GFF6408 0.74 -0.6 83,165 - GFF6408 0.74 -0.4 83,165 - GFF6408 0.74 +0.7 83,165 - GFF6408 0.74 +0.3 83,165 - GFF6408 0.74 -0.2 83,165 - GFF6408 0.74 -0.1 83,194 + GFF6408 0.75 -0.0 83,194 + GFF6408 0.75 -0.0 83,195 - GFF6408 0.75 -0.6 83,195 - GFF6408 0.75 +0.1 83,386 + GFF6408 0.87 -0.1 83,386 + GFF6408 0.87 -0.2 83,386 + GFF6408 0.87 -0.7 83,386 + GFF6408 0.87 +1.4 83,387 - GFF6408 0.87 +0.9 83,387 - GFF6408 0.87 -0.9 83,387 - GFF6408 0.87 +0.1 83,425 + GFF6408 0.89 -0.3 83,603 + +0.7 83,603 + +0.0 83,604 - -0.9 83,604 - +0.6 83,605 + +0.8 83,606 - -0.5 83,632 + +0.4 83,632 + -1.4 83,632 + -0.1 83,632 + -0.0 83,633 - -0.1 83,633 - +0.4 83,633 - +0.5 83,651 - -0.6 83,740 + -0.3 83,740 + +0.2 83,741 - +1.3 84,209 + GFF6409 0.36 -1.0 84,209 + GFF6409 0.36 +0.1 84,209 + GFF6409 0.36 -0.9 84,210 - GFF6409 0.36 +0.3 84,210 - GFF6409 0.36 -0.1 84,210 - GFF6409 0.36 -1.4 84,579 + GFF6409 0.60 -0.6
Or see this region's nucleotide sequence