Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6331 and GFF6332 are separated by 97 nucleotides GFF6332 and GFF6333 are separated by 5 nucleotides
GFF6331 - D-octopine dehydrogenase (EC 1.5.1.11), at 125,578 to 126,648
GFF6331
GFF6332 - Maltodextrin glucosidase (EC 3.2.1.20), at 126,746 to 128,434
GFF6332
GFF6333 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 128,440 to 130,614
GFF6333
Position (kb)
126
127
128
129 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 126.069 kb on + strand, within GFF6331 at 126.070 kb on - strand, within GFF6331 at 126.235 kb on + strand, within GFF6331 at 126.236 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.692 kb on - strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.803 kb on + strand at 126.804 kb on - strand at 126.812 kb on + strand at 126.812 kb on + strand at 126.812 kb on + strand at 126.813 kb on - strand at 126.813 kb on - strand at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.145 kb on + strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.146 kb on - strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.244 kb on + strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.245 kb on - strand, within GFF6332 at 127.247 kb on + strand, within GFF6332 at 127.247 kb on + strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.248 kb on - strand, within GFF6332 at 127.469 kb on + strand, within GFF6332 at 127.469 kb on + strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.470 kb on - strand, within GFF6332 at 127.491 kb on - strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.613 kb on + strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.614 kb on - strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.646 kb on + strand, within GFF6332 at 127.647 kb on - strand, within GFF6332 at 127.647 kb on - strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.874 kb on + strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.875 kb on - strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 127.937 kb on + strand, within GFF6332 at 128.054 kb on + strand, within GFF6332 at 128.054 kb on + strand, within GFF6332 at 128.055 kb on - strand, within GFF6332 at 128.055 kb on - strand, within GFF6332 at 128.387 kb on + strand at 128.686 kb on + strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 128.687 kb on - strand, within GFF6333 at 129.004 kb on + strand, within GFF6333 at 129.004 kb on + strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.005 kb on - strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.178 kb on + strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.179 kb on - strand, within GFF6333 at 129.236 kb on - strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.265 kb on + strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.266 kb on - strand, within GFF6333 at 129.340 kb on + strand, within GFF6333
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 125,807 + GFF6331 0.21 -0.3 125,807 + GFF6331 0.21 -0.7 125,807 + GFF6331 0.21 +0.6 125,807 + GFF6331 0.21 -1.5 125,807 + GFF6331 0.21 -0.8 125,807 + GFF6331 0.21 -0.1 125,807 + GFF6331 0.21 -0.1 125,807 + GFF6331 0.21 -1.1 125,808 - GFF6331 0.21 -0.0 125,808 - GFF6331 0.21 +0.8 125,808 - GFF6331 0.21 -0.6 125,808 - GFF6331 0.21 -0.6 125,808 - GFF6331 0.21 +1.0 125,808 - GFF6331 0.21 -0.2 125,808 - GFF6331 0.21 +0.1 125,920 + GFF6331 0.32 -0.2 125,920 + GFF6331 0.32 -1.7 125,920 + GFF6331 0.32 -0.2 125,920 + GFF6331 0.32 +0.1 125,920 + GFF6331 0.32 -1.3 125,921 - GFF6331 0.32 +0.1 125,921 - GFF6331 0.32 +0.1 125,921 - GFF6331 0.32 -1.0 125,921 - GFF6331 0.32 -0.4 125,921 - GFF6331 0.32 +0.4 126,069 + GFF6331 0.46 +0.2 126,070 - GFF6331 0.46 -0.1 126,235 + GFF6331 0.61 -0.0 126,236 - GFF6331 0.61 +0.6 126,318 - GFF6331 0.69 -0.2 126,318 - GFF6331 0.69 +0.8 126,448 + GFF6331 0.81 +1.4 126,448 + GFF6331 0.81 +0.4 126,448 + GFF6331 0.81 -0.5 126,449 - GFF6331 0.81 +1.2 126,449 - GFF6331 0.81 -1.4 126,449 - GFF6331 0.81 -1.3 126,449 - GFF6331 0.81 -0.2 126,469 + GFF6331 0.83 +0.8 126,469 + GFF6331 0.83 -0.2 126,692 - -0.6 126,803 + +0.3 126,803 + -0.4 126,803 + +0.2 126,803 + +0.0 126,804 - +0.5 126,812 + -0.7 126,812 + +1.8 126,812 + -0.0 126,813 - +0.1 126,813 - -0.2 127,145 + GFF6332 0.24 +0.5 127,145 + GFF6332 0.24 -0.6 127,145 + GFF6332 0.24 -1.1 127,145 + GFF6332 0.24 +1.8 127,145 + GFF6332 0.24 -0.8 127,145 + GFF6332 0.24 -1.3 127,145 + GFF6332 0.24 +0.7 127,145 + GFF6332 0.24 -0.2 127,145 + GFF6332 0.24 -0.6 127,146 - GFF6332 0.24 -2.2 127,146 - GFF6332 0.24 -0.3 127,146 - GFF6332 0.24 -1.1 127,146 - GFF6332 0.24 -0.0 127,244 + GFF6332 0.29 -1.3 127,244 + GFF6332 0.29 +0.4 127,244 + GFF6332 0.29 -0.4 127,244 + GFF6332 0.29 -0.6 127,244 + GFF6332 0.29 +0.3 127,244 + GFF6332 0.29 -0.8 127,245 - GFF6332 0.30 +0.2 127,245 - GFF6332 0.30 -0.2 127,245 - GFF6332 0.30 -0.3 127,247 + GFF6332 0.30 -1.0 127,247 + GFF6332 0.30 -0.4 127,248 - GFF6332 0.30 -0.7 127,248 - GFF6332 0.30 -0.4 127,248 - GFF6332 0.30 -0.1 127,248 - GFF6332 0.30 -0.6 127,248 - GFF6332 0.30 -0.0 127,248 - GFF6332 0.30 -1.0 127,248 - GFF6332 0.30 +0.2 127,248 - GFF6332 0.30 +0.0 127,248 - GFF6332 0.30 +3.8 127,248 - GFF6332 0.30 +0.1 127,248 - GFF6332 0.30 -1.9 127,248 - GFF6332 0.30 -0.4 127,469 + GFF6332 0.43 -1.9 127,469 + GFF6332 0.43 -0.3 127,470 - GFF6332 0.43 +0.1 127,470 - GFF6332 0.43 -0.1 127,470 - GFF6332 0.43 -1.2 127,491 - GFF6332 0.44 +0.3 127,613 + GFF6332 0.51 +0.2 127,613 + GFF6332 0.51 +0.0 127,613 + GFF6332 0.51 -0.5 127,613 + GFF6332 0.51 -0.2 127,614 - GFF6332 0.51 -0.2 127,614 - GFF6332 0.51 -1.1 127,614 - GFF6332 0.51 -0.2 127,614 - GFF6332 0.51 -0.8 127,646 + GFF6332 0.53 -1.6 127,646 + GFF6332 0.53 -1.6 127,646 + GFF6332 0.53 -0.0 127,646 + GFF6332 0.53 -0.0 127,646 + GFF6332 0.53 -1.4 127,646 + GFF6332 0.53 -0.6 127,647 - GFF6332 0.53 +0.0 127,647 - GFF6332 0.53 -0.5 127,874 + GFF6332 0.67 +0.8 127,874 + GFF6332 0.67 +0.9 127,874 + GFF6332 0.67 +0.6 127,874 + GFF6332 0.67 -0.1 127,874 + GFF6332 0.67 -0.8 127,875 - GFF6332 0.67 +0.1 127,875 - GFF6332 0.67 -0.9 127,875 - GFF6332 0.67 -0.4 127,875 - GFF6332 0.67 +0.3 127,875 - GFF6332 0.67 -0.9 127,875 - GFF6332 0.67 +0.5 127,937 + GFF6332 0.71 +1.0 127,937 + GFF6332 0.71 -0.3 127,937 + GFF6332 0.71 +0.7 128,054 + GFF6332 0.77 -0.1 128,054 + GFF6332 0.77 +0.5 128,055 - GFF6332 0.78 +0.1 128,055 - GFF6332 0.78 -0.5 128,387 + +0.8 128,686 + GFF6333 0.11 -0.1 128,687 - GFF6333 0.11 -0.5 128,687 - GFF6333 0.11 -0.4 128,687 - GFF6333 0.11 -0.8 129,004 + GFF6333 0.26 -1.5 129,004 + GFF6333 0.26 -0.6 129,005 - GFF6333 0.26 -0.3 129,005 - GFF6333 0.26 -0.6 129,005 - GFF6333 0.26 -0.7 129,005 - GFF6333 0.26 +0.1 129,178 + GFF6333 0.34 +0.2 129,178 + GFF6333 0.34 +0.2 129,178 + GFF6333 0.34 -0.9 129,178 + GFF6333 0.34 -0.4 129,178 + GFF6333 0.34 -1.0 129,178 + GFF6333 0.34 -0.2 129,178 + GFF6333 0.34 -0.9 129,179 - GFF6333 0.34 +0.0 129,179 - GFF6333 0.34 -2.0 129,179 - GFF6333 0.34 +0.4 129,179 - GFF6333 0.34 +0.2 129,179 - GFF6333 0.34 -0.9 129,236 - GFF6333 0.37 -1.2 129,265 + GFF6333 0.38 +1.2 129,265 + GFF6333 0.38 +0.5 129,265 + GFF6333 0.38 -1.6 129,265 + GFF6333 0.38 +0.0 129,266 - GFF6333 0.38 -0.0 129,266 - GFF6333 0.38 +0.5 129,266 - GFF6333 0.38 -1.5 129,340 + GFF6333 0.41 -0.5
Or see this region's nucleotide sequence