Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6300 and GFF6301 overlap by 8 nucleotides GFF6301 and GFF6302 are separated by 126 nucleotides GFF6302 and GFF6303 are separated by 46 nucleotides
GFF6300 - CaiB/BaiF family protein, at 89,072 to 90,262
GFF6300
GFF6301 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 90,255 to 91,193
GFF6301
GFF6302 - Transcriptional regulator, IclR family, at 91,320 to 92,060
GFF6302
GFF6303 - BUG/TctC family periplasmic protein, at 92,107 to 93,078
GFF6303
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 90.237 kb on + strand at 90.237 kb on + strand at 90.237 kb on + strand at 90.237 kb on + strand at 90.238 kb on - strand at 90.322 kb on + strand at 90.322 kb on + strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.323 kb on - strand at 90.466 kb on + strand, within GFF6301 at 90.466 kb on + strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.467 kb on - strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.646 kb on + strand, within GFF6301 at 90.647 kb on - strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.084 kb on + strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.085 kb on - strand, within GFF6301 at 91.271 kb on - strand at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.473 kb on + strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.474 kb on - strand, within GFF6302 at 91.548 kb on + strand, within GFF6302 at 91.548 kb on + strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.549 kb on - strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.587 kb on + strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.588 kb on - strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.668 kb on + strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.669 kb on - strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.860 kb on + strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.861 kb on - strand, within GFF6302 at 91.998 kb on + strand at 91.998 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.010 kb on + strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.011 kb on - strand at 92.173 kb on + strand at 92.173 kb on + strand at 92.173 kb on + strand at 92.174 kb on - strand at 92.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 89,568 + GFF6300 0.42 +0.2 89,568 + GFF6300 0.42 -0.6 89,568 + GFF6300 0.42 -0.5 89,569 - GFF6300 0.42 -0.5 89,569 - GFF6300 0.42 +0.6 89,569 - GFF6300 0.42 -1.8 89,569 - GFF6300 0.42 +0.2 89,569 - GFF6300 0.42 -0.6 89,569 - GFF6300 0.42 +0.3 89,569 - GFF6300 0.42 -0.8 89,569 - GFF6300 0.42 -0.2 89,569 - GFF6300 0.42 -1.5 89,569 - GFF6300 0.42 -0.3 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 +0.3 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 -0.8 89,818 - GFF6300 0.63 +0.1 89,818 - GFF6300 0.63 -1.5 89,818 - GFF6300 0.63 -0.8 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 -1.0 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 -0.5 89,818 - GFF6300 0.63 +0.3 89,818 - GFF6300 0.63 -0.6 89,871 + GFF6300 0.67 +0.0 89,871 + GFF6300 0.67 +0.4 89,871 + GFF6300 0.67 +0.2 89,904 + GFF6300 0.70 +0.1 89,904 + GFF6300 0.70 +0.3 89,904 + GFF6300 0.70 +1.1 89,904 + GFF6300 0.70 +0.2 89,904 + GFF6300 0.70 -0.3 89,904 + GFF6300 0.70 -0.9 89,904 + GFF6300 0.70 -1.5 89,904 + GFF6300 0.70 -0.6 89,904 + GFF6300 0.70 -0.0 89,904 + GFF6300 0.70 +0.8 89,904 + GFF6300 0.70 -1.0 89,904 + GFF6300 0.70 -0.2 89,904 + GFF6300 0.70 +0.4 89,904 + GFF6300 0.70 +0.4 89,904 + GFF6300 0.70 -0.4 89,904 + GFF6300 0.70 +0.2 89,904 + GFF6300 0.70 -0.9 89,905 - GFF6300 0.70 -0.3 89,905 - GFF6300 0.70 +0.1 89,905 - GFF6300 0.70 -0.2 89,905 - GFF6300 0.70 +0.1 89,905 - GFF6300 0.70 +0.0 89,905 - GFF6300 0.70 +0.8 89,905 - GFF6300 0.70 -0.6 89,905 - GFF6300 0.70 -1.7 89,905 - GFF6300 0.70 +1.0 89,905 - GFF6300 0.70 -0.5 89,905 - GFF6300 0.70 -0.3 89,905 - GFF6300 0.70 +0.5 89,905 - GFF6300 0.70 -0.2 89,905 - GFF6300 0.70 -0.5 89,905 - GFF6300 0.70 +0.2 89,905 - GFF6300 0.70 -0.3 89,940 + GFF6300 0.73 -1.6 89,940 + GFF6300 0.73 +0.6 89,941 - GFF6300 0.73 -0.3 89,941 - GFF6300 0.73 +0.1 89,941 - GFF6300 0.73 +0.1 90,237 + -0.4 90,237 + -0.7 90,237 + -0.2 90,237 + +0.2 90,238 - -0.2 90,322 + +1.1 90,322 + -0.7 90,323 - -0.0 90,323 - -1.2 90,323 - -0.6 90,466 + GFF6301 0.22 -1.0 90,466 + GFF6301 0.22 -1.0 90,467 - GFF6301 0.23 +0.0 90,467 - GFF6301 0.23 -0.4 90,467 - GFF6301 0.23 -0.1 90,646 + GFF6301 0.42 -0.3 90,646 + GFF6301 0.42 +0.1 90,646 + GFF6301 0.42 -0.2 90,646 + GFF6301 0.42 -0.9 90,646 + GFF6301 0.42 -0.5 90,646 + GFF6301 0.42 +0.4 90,646 + GFF6301 0.42 +0.2 90,647 - GFF6301 0.42 -0.8 91,084 + GFF6301 0.88 -0.3 91,084 + GFF6301 0.88 -0.7 91,084 + GFF6301 0.88 -0.2 91,085 - GFF6301 0.88 +0.1 91,085 - GFF6301 0.88 -0.0 91,085 - GFF6301 0.88 -0.8 91,271 - +0.6 91,473 + GFF6302 0.21 -1.8 91,473 + GFF6302 0.21 +0.0 91,473 + GFF6302 0.21 -0.1 91,473 + GFF6302 0.21 -0.8 91,473 + GFF6302 0.21 -0.4 91,473 + GFF6302 0.21 -1.7 91,473 + GFF6302 0.21 -0.4 91,473 + GFF6302 0.21 -0.7 91,473 + GFF6302 0.21 -0.4 91,474 - GFF6302 0.21 -1.1 91,474 - GFF6302 0.21 -0.3 91,474 - GFF6302 0.21 +1.8 91,474 - GFF6302 0.21 -2.0 91,474 - GFF6302 0.21 -1.4 91,474 - GFF6302 0.21 -1.0 91,474 - GFF6302 0.21 -2.6 91,474 - GFF6302 0.21 -0.9 91,474 - GFF6302 0.21 -0.9 91,474 - GFF6302 0.21 -0.1 91,474 - GFF6302 0.21 +0.8 91,474 - GFF6302 0.21 -0.8 91,548 + GFF6302 0.31 -0.6 91,548 + GFF6302 0.31 +0.3 91,549 - GFF6302 0.31 -0.8 91,549 - GFF6302 0.31 +2.2 91,549 - GFF6302 0.31 -0.9 91,587 + GFF6302 0.36 -0.7 91,587 + GFF6302 0.36 +0.2 91,587 + GFF6302 0.36 -0.6 91,587 + GFF6302 0.36 -0.8 91,587 + GFF6302 0.36 +0.6 91,588 - GFF6302 0.36 -0.2 91,588 - GFF6302 0.36 -0.4 91,588 - GFF6302 0.36 -0.7 91,588 - GFF6302 0.36 +0.1 91,668 + GFF6302 0.47 -3.5 91,668 + GFF6302 0.47 +0.2 91,668 + GFF6302 0.47 -0.5 91,668 + GFF6302 0.47 -0.3 91,668 + GFF6302 0.47 +0.2 91,668 + GFF6302 0.47 +1.2 91,669 - GFF6302 0.47 -0.5 91,669 - GFF6302 0.47 -0.0 91,669 - GFF6302 0.47 -1.3 91,669 - GFF6302 0.47 -0.8 91,669 - GFF6302 0.47 -1.0 91,669 - GFF6302 0.47 +0.2 91,669 - GFF6302 0.47 -0.4 91,669 - GFF6302 0.47 -0.3 91,860 + GFF6302 0.73 -0.9 91,860 + GFF6302 0.73 -0.8 91,860 + GFF6302 0.73 -0.3 91,860 + GFF6302 0.73 -0.2 91,860 + GFF6302 0.73 -2.9 91,860 + GFF6302 0.73 +0.8 91,860 + GFF6302 0.73 -1.3 91,861 - GFF6302 0.73 -0.7 91,861 - GFF6302 0.73 +0.1 91,861 - GFF6302 0.73 +0.2 91,861 - GFF6302 0.73 +0.2 91,861 - GFF6302 0.73 -0.6 91,998 + -0.9 91,998 + -0.7 92,010 + -2.6 92,010 + -0.9 92,010 + -0.2 92,010 + -0.3 92,010 + -0.5 92,010 + -0.0 92,010 + +0.1 92,010 + -0.5 92,010 + -0.5 92,011 - +1.0 92,011 - -0.7 92,011 - -0.4 92,011 - -1.6 92,011 - +0.2 92,011 - -0.5 92,173 + -0.0 92,173 + +0.0 92,173 + +0.9 92,174 - -0.3 92,174 - -0.4
Or see this region's nucleotide sequence